Multivalent II [β-d-Galp-(1→4)-β-d-GlcpNAc] and Tα [β-d-Galp-(1→3)-α-d-GalpNAc] specific Moraceae family plant lectin from the seeds of Ficus bengalensis fruits

Characterization, tissue expression, and immunohistochemical localization of MCL3, a C-type lectin produced by Perkinsus olseni-infected Manila clams (Ruditapes philippinarum)

“…Bovine mucin type II completely inhibited agglutination. Owing to their polyvalence, multi-branched oligosaccharides exhibit significantly greater lectin binding reactivity than their linear counterparts [23][24][25]. This could account for the inhibition of rMCL activity by the mannans and mucin type II.…”

Gal/GalNAc specific multiple lectins in marine bivalve Anadara granosa

“…Although monosaccharide affinity of lectins is very specific, it is weaker than that with polyvalent glycotopes. Polyvalent glycotopes with appropriate sugar sequences and required geometry could lead to a great increase of binding affinity with lectins [19,31,34]. Natural polyvalent glycoproteins with well-defined glycotopes were used in the present binding study to mimic the natural phenomenon.…”

Macoma birmanica agglutinin recognizes glycoside clusters of β-GlcNAc/Glc and α-Man

“…12,13 The volume of each reagent added to the 96-well F-bottomed microtiter plates (Maxisorp, NUNC, Denmark) was 50 lL per well. All incubations except coating were performed at 20°C.…”

“…43 MBA was biotinylated by biotinamidocaproate-N-hydroxysuccinimide ester (Sigma, USA) by the method of Duk et al, as described earlier. [11][12][13] Briefly, 200 lg of MBA in 4 mL of 20 mM Tris buffer saline, pH 7.2 (TBS) was incubated with 100 lg biotin ester dissolved in 50 lL methanol, for 30 min at room temperature.…”

“…11 Therefore, in this communication we elaborately described the carbohydrate recognition profile of MBA with oligosaccharides, polysaccharides, and cluster N-linked glycans present in macromolecules by enzyme-linked lectin-sorbent assay (ELLSA) and inhibition assay. 12,13 …”