Multivalent aptasensor array and silver aggregated amplification for multiplex detection in microfluidic devices

Liu, Xiao‐Hui; Li, Hui; Zhao, Yaju; Yu, Xiao-Dong; Xu, Danke

doi:10.1016/j.talanta.2018.05.048

Cited by 15 publications

(11 citation statements)

References 47 publications

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“…The inlet of the right module was utilized to introduce QN-mAb/MB. A mixer was also designed to increase the interactions between QNs and QN-mAb/MB. , In the microfluidic network, both of the target QNs and IS ENR-d5 were captured by the QN-mAb on QN-mAb/MB. To immobilize the QN-mAb/MB, permanent magnets were placed below the magnetic separation chambers.…”

Section: Resultsmentioning

confidence: 99%

“…To perform quantitative analysis, milk samples (0.48×), TCA (1.45%), NaOH solution, PBS (2.4×), and QN-mAb/MB were simultaneously loaded into the microfluidic chip at flow rates of 3.1, 3.1, 5, 5, and 15 μL/min, respectively. A mixer was designed to promote the interaction in the channels. , A micropillar array column was designed to remove aggregated proteins from milk. Then QN-mAb/MB were trapped in the sorting chamber by a permanent magnet (Nd–Fe–B, 3 mm × 3 mm × 13 mm, N45) placed below the chambers.…”

Section: Methodsmentioning

confidence: 99%

“…A mixer was designed to promote the interaction in the channels. 23,24 A micropillar array column was designed to remove aggregated proteins from milk. Then QN-mAb/MB were trapped in the sorting chamber by a permanent magnet (Nd−Fe−B, 3 mm × 3 mm × 13 mm, N45) placed below the chambers.…”

Section: Analytical Chemistrymentioning

confidence: 99%

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Highly Integrated Microfluidic Chip Coupled to Mass Spectrometry for Online Analysis of Residual Quinolones in Milk

et al. 2019

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In this work, a highly integrated microfluidic chip with multifunction coupled to mass spectrometry (MS) was developed for online analysis of seven different regulated quinolones (QNs) in milk samples. Procedures of sample extraction, immunoaffinity enrichment, magnetic separation, and online elution were performed simultaneously on the specifically designed device. Based on the specificity of antibodies, direct (electrospray ionization) ESI-MS at full scan mode without liquid chromatography (LC) separation and further tandem mass spectrometry (MS/MS) analysis was developed for the identification of target QNs. One single isotope internal standard (IS) method was presented for quantitative analysis of seven QNs. Upon targeted online extraction and enrichment by antibody conjugated magnetic beads, seven QNs were quantitatively determined by the IS method with the linear range of 0.2/0.5–10 ng/mL (R 2 > 0.991). The limits of detection (LODs) for the seven QNs were in the range of 0.047–0.490 ng/mL. This system permits automated on-chip immunoaffinity enrichment and accurate MS detection without additional off-line cleanup procedures.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Highly Integrated Microfluidic Chip Coupled to Mass Spectrometry for Online Analysis of Residual Quinolones in Milk

et al. 2019

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“…Liu et al . developed a rapid and sensitive assay in a microfluidic device based on a multivalent aptasensor array chip and silver aggregated amplification for detection of proteins platelet‐derived growth factor‐BB and vascular endothelial growth factor‐165 by fluorescence. The multiplexed detection of three different biomarker genes relevant to a breast cancer diagnosis was performed by Veselinovic and co‐workers through square wave voltammetry.…”

Section: Introductionmentioning

confidence: 94%

New Disposable Electrochemical Paper‐based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample

et al. 2020

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A disposable electrochemical paper‐based analytical device was constructed based on use of sequential analysis with multiplexed working electrodes and applied for the determination of glucose, creatinine, and uric acid. The device was constructed with 16 microfluidic channels, with 16 working electrodes arranged in four set with four components surrounding the sample injection hole. In addition, a commercial multiplexing module was used, which allowed for multiplexing of the 16 working electrodes. This design allowed for radial and homogeneous sample elution to each sensing spot for high throughput analysis. In the multiplexed determinations, distinct electrochemical procedures were employed for each analyte. Furthermore, each working electrode spot was modified to increase the respective analytical signals. For glucose detection, the sensor was based on electron mediation by ferrocenecarboxylic acid over the modified surface with glucose oxidase. The principle for creatinine detection was based on electrochemical reduction of non‐complexed Fe3+ in excess after complex formation between Fe3+ and creatinine in the chemical step. The anodic peak current responses for uric acid detection increased due to working electrode surface modification with carbon black nanoparticles. In the multiplexed analysis, the device provided limits of detection of 0.120 mmol L−1, 0.084 mmol L−1, and 0.012 mmol L−1 for glucose, creatinine, and uric acid, respectively. The developed device was successfully applied in the analyses of real urine samples.

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“…25 Furthermore, PCR amplification bias and reduced library diversity due to experimental operation could also affect the binding properties of aptamers. Thus, apart from truncation, several other post-SELEX optimization methods have also been adopted, such as multivalent aptamers, 27 chemical modification, 28,29 and random or site-directed mutagenesis. 30 The application of these post-SELEX optimization methods significantly improved the binding affinity of the aptamers, indicating that post-SELEX optimization could make up for the drawbacks of SELEX.…”

Section: ■ Introductionmentioning

confidence: 99%

Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus Determination

Sun

Duan

et al. 2019

J. Agric. Food Chem.

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In this work, after optimizing the original aptamer sequence by truncation and site-directed mutagenesis, a simple and sensitive colorimetric aptasensor was established for detecting the widespread food-borne pathogen Vibrio parahemolyticus (V. parahemolyticus). The detection strategy was based on the competition for an V. parahemolyticus specific aptamer between its complementary DNA (cDNA) and V. parahemolyticus. The aptamer-conjugated magnetic nanoparticles (MNPs) were used as capture probes, and the G-quadruplex (G4) DNAzyme was employed as the signal amplifying element. Under optimal conditions, a wide linear detection range (from 102 to 107 cfu/mL) was available, and the detection limit could be as low as 10 cfu/mL. This method was also used to detect V. parahemolyticus in contaminated salmon samples, and the results showed good consistency with those obtained from standard plate counting method. Therefore, this novel aptasensor could be a good candidate for sensitive and selective detection of V. parahemolyticus without complicated operations.

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Multivalent aptasensor array and silver aggregated amplification for multiplex detection in microfluidic devices

Cited by 15 publications

References 47 publications

Highly Integrated Microfluidic Chip Coupled to Mass Spectrometry for Online Analysis of Residual Quinolones in Milk

Highly Integrated Microfluidic Chip Coupled to Mass Spectrometry for Online Analysis of Residual Quinolones in Milk

New Disposable Electrochemical Paper‐based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample

Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus Determination

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