Multistep enzyme cascades as a route towards green and sustainable pharmaceutical syntheses

Microbial Lipases and Their Potential in the Production of Pharmaceutical Building Blocks

“…Finally, although microbial lipases could be considered one of the most versatile, they alone can hardly reach the ideal goal of a predominant enzyme synthesis of an API, especially if they involve redox steps; thus, it is necessary to explore the development of enzyme cascades which include the aspects previously listed [ 236 , 237 , 238 , 239 ]. Indeed, the implementation of enzyme cascades in the synthesis of APIs with possibilities of industrial exploitation were recently reviewed [ 240 ].…”

Putting precision and elegance in enzyme immobilisation with bio-orthogonal chemistry

“…8,257 This could be used, for example, to drive in vitro multi-enzyme cascade processes, in emulation of the exquisite orchestration of metabolic pathways in vivo, and provide truly sustainable biocatalytic methods for the industrial production of chemicals. [257][258][259][260][261]…”

Combined chemoenzymatic strategy for sustainable continuous synthesis of the natural product hordenine

“…Several examples have been reported on the synthesis of complex molecules which can be achieved with high efficiency and increased sustainability, successfully assembling fully enzymatic cascade reactions in continuous mode, mimicking biosynthetic pathways or, albeit yet less established, combining chemoenzymatic reactors in line. 40,41 By compartmentalizing each step in separated flow reactors, we designed a simple chemoenzymatic cascade in continuous flow mode (Figure 2), obtaining hordenine as a valuable natural product. The naturally abundant starting material L-tyrosine is here converted into tyramine by the immobilised tyrosine decarboxylase from Lactobacillus brevis (LbTDC) 42 , and then a chemical reductive amination follows in-line.…”