Multistage and transmission-blocking tubulin targeting potent antimalarial discovered from the open access MMV pathogen box

Host-Erythrocytic Sphingosine-1-Phosphate Regulates Plasmodium Histone Deacetylase Activity and Exhibits Epigenetic Control over Cell Death and Differentiation

“…6B , panel I). The cellular localization of tubulin was consistent with our previous findings ( 37 , 38 ). The fluorescence intensities were plotted as a bar graph by measuring 10 individual cells each from the PF-543-treated and untreated samples ( Fig.…”

Targeting an essential Plasmodium cold shock protein to block growth and transmission of malaria parasite

“…Pf -tubulin polymerization was carried out in 100 μL of a ice-cold re-assembly buffer (100 mM PIPES, pH 6.9, 10% glycerol, 2 mM MgCl 2 .H2O, 0.5 mM EGTA), containing 1 mM GTP, and Pf alpha and Pf β-tubulins at 10 μM concentration. 51 The reaction mixture was prepared in clear, flat bottom 96 wells plates. The time-course of tubulin polymerization was followed over 30 min at 37°C by monitoring a change in turbidity at 350 nm using a Varioskan LUX multimode microplate reader (Thermo Scientific) equipped with an incubator for temperature control.…”