Multispectral photoacoustic microscopy of lipids using a pulsed supercontinuum laser

Buma, Takashi; Conley, Nicole C.; Choi, Sang Won

doi:10.1364/boe.9.000276

Cited by 41 publications

(33 citation statements)

References 35 publications

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“…The SC source, which is based on 3 meters of standard telecom range SMF and a diode laser-based amplifier, is designed to have its maximum PED of 25 nJ/nm in the center of the region at 1725-1775 nm. This is factor >3 times higher than the so far record PED used for PAI in this spectral region [18]. The output PED of the SC can further be scaled by using additional amplifiers or pump sources with higher peak powers, with the scaling factor ultimately limited by the damage threshold of the fiber [16].…”

Section: Resultsmentioning

confidence: 92%

“…Unfortunately, such an SC will then have low PED because the Raman lines take most of the energy [15,17]. Nevertheless, such a Raman SC source was recently used in a very nice study on multispectral PAI of lipids [18]. The source used a 1047 nm based Nd: YLF Q-switched laser to pump a photonic crystal fiber (PCF) with a ZDW at 1200 nm to generate Raman lines at 1098 and 1153 nm, and then an SC from 1200 to 1800 nm.…”

Section: Introductionmentioning

confidence: 99%

“…The low energy meant that PAI could not be done above 1714 nm, i.e. could not cover the most important wavelength of 1720 nm of maximum absorption of lipids [18]. In addition, a long length of 30 meters PCF (at $194/meter, price from Thorlabs) had to be used, which makes the source very expensive.…”

Section: Introductionmentioning

confidence: 99%

“…Importantly the state-of-the-art SC-based PAI studies [18] imply that spectroscopic PAI of lipids in the first overtone region (i.e. with more than 1 wavelength), has not yet been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

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High-pulse energy supercontinuum laser for high-resolution spectroscopic photoacoustic imaging of lipids in the 1650-1850 nm region

Dasa¹,

Markos²,

Maria³

et al. 2018

Biomed. Opt. Express

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We propose a cost-effective high-pulse energy supercontinuum (SC) source based on a telecom range diode laser-based amplifier and a few meters of standard single-mode optical fiber, with a pulse energy density as high as ~25 nJ/nm in the 1650-1850 nm regime (factor >3 times higher than any SC source ever used in this wavelength range). We demonstrate how such an SC source combined with a tunable filter allows high-resolution spectroscopic photoacoustic imaging and the spectroscopy of lipids in the first overtone transition band of C-H bonds (1650-1850 nm). We show the successful discrimination of two different lipids (cholesterol and lipid in adipose tissue) and the photoacoustic cross-sectional scan of lipid-rich adipose tissue at three different locations. The proposed high-pulse energy SC laser paves a new direction towards compact, broadband and cost-effective source for spectroscopic photoacoustic imaging.

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Section: Resultsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High-pulse energy supercontinuum laser for high-resolution spectroscopic photoacoustic imaging of lipids in the 1650-1850 nm region

Dasa¹,

Markos²,

Maria³

et al. 2018

Biomed. Opt. Express

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show abstract

“…To achieve a cost-efficient excitation laser for lipid imaging, Buma et al developed a multispectral PAM for lipid imaging with a pulsed-supercontinuum laser [ 33 ]. The 1225 and 1714 nm wavelengths were used to highlight lipids, while 1325 and 1600 nm wavelengths were selected to provide image contrast for water.…”

Section: Endogenous Contrast Agentsmentioning

confidence: 99%

A Review of Endogenous and Exogenous Contrast Agents Used in Photoacoustic Tomography with Different Sensing Configurations

Tsang

Wong

2020

Sensors

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Optical-based sensing approaches have long been an indispensable way to detect molecules in biological tissues for various biomedical research and applications. The advancement in optical microscopy is one of the main drivers for discoveries and innovations in both life science and biomedical imaging. However, the shallow imaging depth due to the use of ballistic photons fundamentally limits optical imaging approaches’ translational potential to a clinical setting. Photoacoustic (PA) tomography (PAT) is a rapidly growing hybrid imaging modality that is capable of acoustically detecting optical contrast. PAT uniquely enjoys high-resolution deep-tissue imaging owing to the utilization of diffused photons. The exploration of endogenous contrast agents and the development of exogenous contrast agents further improve the molecular specificity for PAT. PAT’s versatile design and non-invasive nature have proven its great potential as a biomedical imaging tool for a multitude of biomedical applications. In this review, representative endogenous and exogenous PA contrast agents will be introduced alongside common PAT system configurations, including the latest advances of all-optical acoustic sensing techniques.

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Multimodal photoacoustic imaging in analytic vulnerability of atherosclerosis

Zhou,

Chen,

et al. 2023

iRADIOLOGY

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Atherosclerosis is a major cause of mortality and morbidity, and rupture can lead to myocardial infarction and stroke. The vulnerability assessment of atherosclerosis plaques is important for providing medical treatment. Identifying vulnerable plaques requires noninvasive, high‐resolution imaging techniques for capturing and locating high‐risk markers. Photoacoustic imaging (PAI) is a hybrid and novel imaging modality that uses nonionizing excitation and has substantial promise for vulnerability assessment of atherosclerosis. The current review examined current applications of multimodal PAI for identification of atherosclerosis plaques. Furthermore, we discuss the challenges and limitations in the implementation this method and future research directions to overcome these difficulties. First, we discuss two aspects of vulnerable plaque characterization: large necrotic cores and thin degraded cap component identification. Then, we then discuss applications of high‐risk plaque characterization, including intraplaque hemorrhage and heme degradation markers detection. We hope that this review will shed light on the potential application of optical imaging techniques in atherosclerosis identification and facilitate further studies that will ultimately lead to the broader application of multimodal PAI in clinical practice.

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Multispectral photoacoustic microscopy of lipids using a pulsed supercontinuum laser

Cited by 41 publications

References 35 publications

High-pulse energy supercontinuum laser for high-resolution spectroscopic photoacoustic imaging of lipids in the 1650-1850 nm region

High-pulse energy supercontinuum laser for high-resolution spectroscopic photoacoustic imaging of lipids in the 1650-1850 nm region

A Review of Endogenous and Exogenous Contrast Agents Used in Photoacoustic Tomography with Different Sensing Configurations

Multimodal photoacoustic imaging in analytic vulnerability of atherosclerosis

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