Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo

Razansky, Daniel; Distel, Martin; Vinegoni, Claudio; Ma, Rui; Perrimon, Norbert; Köster, Reinhard W.; Ntziachristos, Vasilis

doi:10.1038/nphoton.2009.98

Cited by 621 publications

(517 citation statements)

References 29 publications

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“…10,11,13 Recently developed photoacoustic tomography (PAT) has been reported to be able to image fluorescent molecules with large depth-to-resolution ratio. 14,15 However, the fluorescence measurement is indirect because PAT is based on the optical absorption property of fluorophores. Therefore, it cannot take advantages of the unique emission properties of fluorophores, such as fluorescence lifetimes and emission spectra.…”

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confidence: 99%

High-resolution imaging in a deep turbid medium based on an ultrasound-switchable fluorescence technique

Yuan

Uchiyama

Liu

et al. 2012

Applied Physics Letters

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The spatial resolution of fluorescence imaging techniques in deep optically turbid media such as tissues is limited by photon diffusion. To break the diffusion limit and achieve high-resolution and deep-tissue fluorescence imaging, a fundamentally different method was demonstrated based on a concept of ultrasound-switchable fluorescence. The results showed that a small fluorescent tube with a diameter of $180 lm at a depth of $20 mm in an optical scattering medium (l 0 s % 3:2 and l a % 0:026 cm À1 ) can be clearly imaged with a size of $260 lm. The depth-to-resolution ratio is shown to be about one order of magnitude better than other deep-tissue fluorescence imaging techniques. V C 2012 American Institute of Physics. [http://dx

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mentioning

confidence: 99%

High-resolution imaging in a deep turbid medium based on an ultrasound-switchable fluorescence technique

Yuan

Uchiyama

Liu

et al. 2012

Applied Physics Letters

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“…The large penetration depths and the relatively high resolution of $100 mm (note that this resolution is one tenth of the transport mean free path, $1 mm) make this approach ideal for macroscopic imaging of small animals [31][32][33]. Higher resolution might be achieved with optoacoustic tomography, as seen in Figure 1B-D where Razansky et al [34] show how MSOT can be used to resolve fluorophores in the brain of an adult transgenic zebrafish in vivo with a scalable spatial resolution of a few tenths of microns. In addition, Gateau et al have shown that 3D optoacoustic tomography enables imaging depths of several millimeters to centimeters with scalable resolution under $100 mm [35].…”

Section: Optoacoustic Imagingmentioning

confidence: 99%

Unleashing Optics and Optoacoustics for Developmental Biology

Ripoll

Koberstein-Schwarz

Ntziachristos

2015

Trends in Biotechnology

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“…For this purpose, it is sufficient to generate one or several two-dimensional tomographic images by focusing into selected slices. Such section images have been obtained either with a single detector scanning around a sample [26][27][28][29] or with a circular array [30,31]. In this study, in order to take advantage of the favorable properties of the optical detector and to implement the laser US imaging capability, we present a section imaging setup consisting of a single, optimized detector.…”

Section: Introductionmentioning

confidence: 99%

Hybrid photoacoustic and ultrasound section imaging with optical ultrasound detection

Nuster

Schmitner

Wurzinger

et al. 2013

Journal of Biophotonics

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Journal of BIOPHOTONICSA setup is proposed that provides perfectly co-registered photoacoustic (PA) and ultrasound (US) section images. Photoacoustic and ultrasound backscatter signals are generated by laser pulses coming from the same laser system, the latter by absorption of some of the laser energy on an optically absorbing target near the imaged object. By measuring both signals with the same optical detector, which is focused into the selected section by use of a cylindrical acoustic mirror, the information for both images is acquired simultaneously. Co-registered PA and US images are obtained after applying the inverse Radon transform to the data, which are gathered while rotating the object relative to the detector. Phantom experiments demonstrate a resolution of 1.1 mm between the sections of both imaging modalities and a inplane resolution of about 60 mm and 120 mm for the US and PA modes, respectively. The complementary contrast mechanisms of the two modalities are shown by images of a zebrafish.Result of the dual-modality section imaging experiment on a zebrafish. Scale bar: 1 mm. The first row shows the photoacoustic (PA) and the laser ultrasound (LUS) section image. The second row shows the fusion image (FI) and the histological section.

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Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo

Cited by 621 publications

References 29 publications

High-resolution imaging in a deep turbid medium based on an ultrasound-switchable fluorescence technique

High-resolution imaging in a deep turbid medium based on an ultrasound-switchable fluorescence technique

Unleashing Optics and Optoacoustics for Developmental Biology

Hybrid photoacoustic and ultrasound section imaging with optical ultrasound detection

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