Unleashing Optics and Optoacoustics for Developmental Biology

Ripoll, Jorge; Koberstein-Schwarz, Benno; Ntziachristos, Vasilis

doi:10.1016/j.tibtech.2015.08.002

Cited by 21 publications

(22 citation statements)

References 87 publications

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“…Spatial deconvolution algorithms then exploit the PSF to improve the resolution and the signal-to-noise ratio (SNR) of the images captured. This technique is broadly applied in conventional white-light and fluorescence microscopy, nonlinear and Raman-based microscopy, as well as infrared microscopy and optical coherence tomography [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] . Optical-resolution optoacoustic (photoacoustic) microscopy is considered to complement the contrast of optical microscopy, because it resolves optical absorption [16][17][18][19][20][21][22] .…”

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confidence: 99%

Pushing the boundaries of optoacoustic microscopy by total impulse response characterization

et al. 2020

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Optical microscopy improves in resolution and signal-to-noise ratio by correcting for the system's point spread function; a measure of how a point source is resolved, typically determined by imaging nanospheres. Optical-resolution optoacoustic (photoacoustic) microscopy could be similarly corrected, especially to account for the spatially-dependent signal distortions induced by the acoustic detection and the time-resolved and bi-polar nature of optoacoustic signals. Correction algorithms must therefore include the spatial dependence of signals' origins and profiles in time, i.e. the four-dimensional total impulse response (TIR). However, such corrections have been so far impeded by a lack of efficient TIRcharacterization methods. We introduce high-quality TIR determination based on spatiallydistributed optoacoustic point sources (SOAPs), produced by scanning an optical focus on an axially-translatable 250 nm gold layer. Using a spatially-dependent TIR-correction improves the signal-to-noise ratio by >10 dB and the axial resolution by~30%. This accomplishment displays a new performance paradigm for optoacoustic microscopy.

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Pushing the boundaries of optoacoustic microscopy by total impulse response characterization

et al. 2020

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“…In response, hybrid optoacoustic and optical imaging methods were developed in order to expand the time window of developmental observations [323]. Examples of the recently showcased combinations include optoacoustic and confocal microscopy [25] as well as optoacoustic tomography and selective plane illumination microscopy [324].…”

Section: Applications Enabled By Optoacoustic Visualization Of Multmentioning

confidence: 99%

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

et al. 2017

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Visualization of dynamic functional and molecular events in an unperturbed in vivo environment is essential for understanding the complex biology of living organisms and of disease state and progression. To this end, optoacoustic (photoacoustic) sensing and imaging have demonstrated the exclusive capacity to maintain excellent optical contrast and high resolution in deep-tissue observations, far beyond the penetration limits of modern microscopy. Yet, the time domain is paramount for the observation and study of complex biological interactions that may be invisible in single snapshots of living systems. This review focuses on the recent advances in optoacoustic imaging assisted by smart molecular labeling and dynamic contrast enhancement approaches that enable new types of multiscale dynamic observations not attainable with other bio-imaging modalities. A wealth of investigated new research topics and clinical applications is further discussed, including imaging of large-scale brain activity patterns, volumetric visualization of moving organs and contrast agent kinetics, molecular imaging using targeted and genetically expressed labels, as well as three-dimensional handheld diagnostics of human subjects.

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“…PAT complements other imaging methods in contrast mechanism, spatial-temporal resolution, and penetration depth, and has found broad applications in the biomedical research, especially in functional brain mapping [48], cancer diagnosis and staging [45, 49], tissue engineering and regenerative medicine [50], developmental biology [51], and molecular cell biology [52], as comprehensively reviewed elsewhere [6, 53, 54]. In particular, PAT has been widely used for various cancer studies [45], including fundamental research of cancerogenesis [55], cancer detection and staging [56], and navigation and evaluation in cancer treatment [57].…”

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confidence: 99%

Recent progress in photoacoustic molecular imaging

Yao

Wang

2018

Current Opinion in Chemical Biology

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By acoustically detecting the optical absorption contrast, photoacoustic (PA) tomography (PAT) has broken the penetration limits of traditional high-resolution optical imaging. Through spectroscopic analysis of the target's optical absorption, PAT can identify a wealth of endogenous and exogenous molecules and thus is inherently capable of molecular imaging with high sensitivity. PAT's molecular sensitivity is uniquely accompanied by non-ionizing radiation, high spatial resolution, and deep penetration in biological tissues, which other optical imaging modalities cannot achieve yet. In this concise review, we summarize the most recent technological advancements in PA molecular imaging and highlight the novel molecular probes specifically made for PAT in deep tissues. We conclude with a brief discussion of the opportunities for future advancements.

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Unleashing Optics and Optoacoustics for Developmental Biology

Abstract: This is a postprint version of the following published document: Ripoll, J.; Koberstein-Schwarz, B.; Ntziachristos, V. Unleashing optics and optoacoustics for developmental biology.

Cited by 21 publications

References 87 publications

Pushing the boundaries of optoacoustic microscopy by total impulse response characterization

Pushing the boundaries of optoacoustic microscopy by total impulse response characterization

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

Recent progress in photoacoustic molecular imaging

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