Multiresidue Screening Methods for the Determination of Pesticides in Tomatoes

Moraes, Solange Leite de; Rezende, Maria Olímpia de Oliveira; Nakagawa, Lia Emi; Luchini, Luiz Carlos

doi:10.1081/pfc-120023518

Cited by 10 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Alíquotas de 5,0 µL de soluções de diazinon nas concentrações de 5,0 µg.mL -1 , 8,0 µg.mL -1 , 9,0 µg.mL -1 e 10,0 µg.mL -1 , de diclorvós nas concentrações de 20,0 µg.mL -1 , 100,0 µg.mL -1 , 150,0 µg.mL -1 e 200,0 µg.mL -1 , de alfa-cipermetrina nas concentrações de 5,0 µg.mL -1 , 6,0 µg.mL -1 , 8,0 µg.mL -1 e 10,0 µg.mL -1 , de deltametrina nas concentrações de 5,0 µg.mL -1 , 8,0 µg.mL -1 , 9,0 µg.mL -1 e 10,0 mg.mL -1 , de carbofuran nas concentrações de 0,25 µg.mL -1 , 0,5 µg.mL -1 , 0,7 µg.mL -1 e 0,8 µg.mL -1 e de carbaril nas concentrações de 0,2 µg.mL -1 , 0,3 µg.mL -1 , 0,4 µg.mL -1 e 0,6 µg.mL -1 foram aplicadas em placas de cromatografia em camada delgada preparadas em laboratório tendo como adsorvente sílica gel G Merck. Uma mistura de clorofórmio:acetato de etila (9:1v/v) foi utilizada para a eluição dos praguicidas na placa de cromatografia em camada delgada e a visualização dos praguicidas na placa foi feita por um tratamento com cloro e reagente de orto-toluidina conforme metodologia descrita por MORAES et al (2003). A quantidade mínima visível na placa de cromatografia, considerada a quantidade mínima detectável (QMD), determinou a sensibilidade do método para a detecção de cada composto.…”

Section: Comunicação Científicaunclassified

“…Portanto, a cromatografia em camada delgada foi menos sensível na detecção de diazinon, diclorvós, alfa-cipermetrina e deltametrina e mais sensível na detecção de carbofuran e cabaril. Este resultado também foi observado por MORAES et al (2003) que obtiveram valores de quantidade mínima detectável de 50 ng de carbaril e 125 ng de carbofuran, utilizando o mesmo método. cromatografia em camada delgada é um método analítico que permite a identificação destes compostos.…”

Section: Comunicação Científicaunclassified

See 1 more Smart Citation

Análise De Praguicidas Por Bioensaio Com Mosca Drosophila Melanogaster E Cromatografia Em Camada Delgada

Narciso

Nakagawa

2009

Arq. Inst. Biol.

View full text Add to dashboard Cite

RESUMO A intoxicação de animais por praguicidas é diagnosticada por um conjunto de avaliações, entre elas, a detecção destas substâncias no próprio animal ou em amostras ambientais. Neste trabalho, verificou-se a sensibilidade do bioensaio com Drosophila melanogaster e da cromatografia em camada delgada na detecção dos praguicidas diazinon, diclorvós, alfa-cipermetrina, deltametrina, carbofuran e carbaril. No bioensaio, moscas D. melanogaster foram expostas a diferentes concentrações dos praguicidas para determinar a quantidade capaz de provocar 70% de mortalidade (CL70). Na análise por cromatografia em camada delgada, soluções dos praguicidas foram aplicadas em placas cromatográficas e tratadas com reagente de orto-toluidina para determinação da quantidade mínima detectável. Os valores de CL70 obtidos no bioensaio foram de 0,6?g.mL-1 para o diazinon, 0,14 ?g.mL-1 para o diclorvós, 1,5 ?g.mL-1 para a alfa-cipermetrina, 1,0 ?g.mL-1 para a deltametrina, 5,0 ?g.mL-1 para o carbofuran e 21.000 ?g.mL-1 para o carbaril. As quantidades mínimas detectáveis pela cromatografia em camada delgada foram 20,0 ?g.mL-1, 200 ?g.mL-1, 10,0 ?g.mL-1, 10 ?g.mL-1, 1,0 ?g.mL-1 e 0,4 ?g.mL-1 para diazinon, diclorvós, alfa-cipermetrina, deltametrina, carbofuran e carbaril, respectivamente. Embora a sensibilidade dos dois métodos na detecção dos praguicidas tenha sido bastante variável, ambos podem ser utilizados como métodos de triagem e identificação destes compostos, tendo como vantagem o baixo custo e a simplicidade de execução, podendo ser implantados em laboratórios com poucos recursos.

show abstract

Section: Comunicação Científicaunclassified

Análise De Praguicidas Por Bioensaio Com Mosca Drosophila Melanogaster E Cromatografia Em Camada Delgada

Narciso

Nakagawa

2009

Arq. Inst. Biol.

View full text Add to dashboard Cite

show abstract

“…Numerous analytical methods have been reported for the determination of pesticide residues in various fruits and vegetables (Tsipi et al, 1999;Safi et al, 2002;Martinez Vidal et al, 2002a;Kondo et al, 2003;Moraes et al, 2003;Zrostlikova et al, 2003;Chu et al, 2005). Most of these methods have employed gas chromatography (GC), either with electron-capture detection, nitrogen-phosphorus detection or flame photometric detection (Harry and Pylypiw, 1993;Doong and Lee, 1999;Tsipi et al, 1999;Safi et al, 2002).…”

Section: Introductionmentioning

confidence: 97%

Comparison of different extraction methods for the simultaneous determination of pesticide residues in kiwi fruit using gas chromatography–mass spectrometry

Cho

El‐Aty

Jeon³

et al. 2008

Biomedical Chromatography

View full text Add to dashboard Cite

The methods of simultaneous extraction of iprodione, chlorpyrifos-methyl, EPN and endosulfan (with its metabolites) from kiwi fruit using accelerated solvent extraction (ASE), supercritical fluid extraction (SFE), and liquid-liquid extraction (LLE) were tested and compared in terms of their of limits of detection and quantification, as well as the highest pesticide recoveries with the lowest residues in the final extracts. The analysis was performed using gas chromatography-mass spectrometry in the selected ion monitoring mode. The proposed methods featured good sensitivity, pesticide quantification limits were low enough, and the precision (expressed as relative standard deviations) ranged from 0.56 to 7.17%. The recoveries obtained from ASE, SFE and LLE were 77.5-120, 71.9-109.1 and 75.6-127.1%, respectively. The proposed methods were successfully applied for the monitoring of the selected pesticide residues in kiwi fruit samples collected from Jollanamdo area, Republic of Korea. Iprodione was detected at a level lower than the maximum residue limit (MRL) established by the Korea Food and Drug Administration (5 ppm), while EPN was detected at a level higher than the Korea Food and Drug Administration MRL (0.1 ppm) in the real samples. The proposed sample preparations led to a higher preconcentration of the pesticide fraction, and allowed the sensitive and selective determination of pesticides with varied physicochemical properties in kiwi fruit.

show abstract

“…The development of optimized multiresidue methods for monitoring pesticide residues is highly demanded, and various analytical methods have been reported for the determination of pesticide residues in fruits and vegetables [7][8][9][10]. The determination of pesticides in foods is a difficult task owing to the complexity of the matrices, their different physicochemical properties and the low concentrations in which these compounds are usually present.…”

Section: Introductionmentioning

confidence: 99%

Pesticides in seaweed: optimization of pressurized liquid extraction and in-cell clean-up and analysis by liquid chromatography–mass spectrometry

et al. 2012

View full text Add to dashboard Cite

Chemical residues, such as insecticides and anthelmintics, are frequently redistributed from the aquatic environment to marine species. This work reports on a fast validated protocol for the analysis of azamethiphos, three avermectins, two carbamates and two benzoylurea pesticides and chemotherapeutic agents in seaweeds based on pressurized liquid extraction and separation of analytes by liquid chromatography coupled with tandem mass spectrometry. The variables affecting the efficiency of pressurized liquid extraction, including temperature, number of extraction cycles, static extraction time and percent acetonitrile flush volume, were studied using a Doehlert design. The optimum parameters were 100 °C and one cycle of 3 min with 70 % acetonitrile. Adequate in-cell clean-up of the seaweeds was achieved using 0.8 g of Florisil over 0.1 g of graphitized carbon black on the bottom of the cell. The optimized method was validated using an analyte-free seaweed sample fortified at different concentrations. The limits of quantification ranged from 3.6 μg kg(-1) (azamethiphos) to 31.5 μg kg(-1) (abamectin). The recovery was from 87 to 120 % in most cases at different spiking levels. Finally, the reproducibility of the method expressed as the relative standard deviation and evaluated at concentrations of 10 and 50 μg kg(-1) was in the range 9-14.3 % and 6.1-12.3 %, respectively. The applicability of the method was evaluated with five commercial and 12 wild edible seaweeds, and four target compounds were detected in two wild seaweeds at a concentration below the quantification limit.

show abstract

Multiresidue Screening Methods for the Determination of Pesticides in Tomatoes

Cited by 10 publications

References 10 publications

Análise De Praguicidas Por Bioensaio Com Mosca Drosophila Melanogaster E Cromatografia Em Camada Delgada

Análise De Praguicidas Por Bioensaio Com Mosca Drosophila Melanogaster E Cromatografia Em Camada Delgada

Comparison of different extraction methods for the simultaneous determination of pesticide residues in kiwi fruit using gas chromatography–mass spectrometry

Pesticides in seaweed: optimization of pressurized liquid extraction and in-cell clean-up and analysis by liquid chromatography–mass spectrometry

Contact Info

Product

Resources

About