Multiresidue method for the analysis of multiclass pesticides in agricultural products by gas chromatography-tandem mass spectrometry

Upadhyay

Arabian Journal of Chemistry

et al. 2015

Acephate and its metabolite methamidophos are common organophosphorus insecticide used for crop protection. High uses of acephate and methamidophos have induced health issues and environmental pollution. Their undesired presence in the environment is creating ecotoxicology and may harm human health. It is therefore essential to detect the presence of acephate and methamidophos even in trace level. In this review, we have tried to accommodate successful methods of detection of acephate and methamidophos in the different biological media. Their recovery and residue analysis in different media such as vegetables, human and animal tissues have also included. The most common method for their determination is based on chromatographic separation and identification. Among different chromatographic methods, LC and GC coupled with different detectors have used. But, they both need extensive pretreatment and cleanup procedure, before undergoing chromatographic separation and identification. LC coupled with mass spectrometry (LCMS) is sometime able to detect acephate and methamidophos in ppm level. ª 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Section: Determination Of Acephate and Methamidophos In Vegetablesmentioning

confidence: 96%

A review on sample preparation and chromatographic determination of acephate and methamidophos in different samples

Upadhyay

Arabian Journal of Chemistry

et al. 2015

“…Cui et al (2006) have reported the effect of chlorpyrifos and cypermethrin due to the formation of DNA adducts in primary mouse hepatocytes using bioluminescence assay. The most analytical techniques for cypermethrin detection are based on chromatography and enzyme-linked immunosorbent assay which require trained manpower and are timeconsuming (Janghel et al 2007;Lee et al 2004;Armenta et al 2005;Aguera et al 2002;Vazquez et al 2005;Jin and Webster 1998). Interestingly, Saxena et al (2005) have investigated the effect of cypermethrin on root meristem cells of Allium sativum and Allium cepa using ultraviolet (UV) and Fourier transform infrared (FTIR) spectroscopy techniques, respectively.…”

Section: Introductionmentioning

confidence: 99%

Self-assembled monolayer for toxicant detection using nucleic acid sensor based on surface plasmon resonance technique

Solanki

Prabhakar

Pandey

et al. 2008

Biomed Microdevices

Double stranded calf thymus deoxyribonucleic acid (dsCT-DNA) has been covalently immobilized onto self-assembled monolayer (SAM) of beta-merceptoethanol (MCE) on gold substrates via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry for fabrication of the surface plasmon resonance (SPR) based biosensing device. The dsCT-DNA-MCE-SAM/Au bioelectrode has been characterized using electrochemical impedance spectroscopy, cyclic voltammetry, contact angle measurements and atomic force microscopy, respectively. This biosensing device has been utilized for detection of cypermethrin (0.0005 ppm) using electrochemical and SPR techniques, respectively. The mechanism of interaction of cypermethrin with dsCT-DNA immobilized onto MCE-SAM has been proposed.

“…Thus, the development of analytical methodologies for monitoring pesticide residues in food, specifically of carrots, is an important subject of research in order to facilitate food quality evaluation, thereby avoiding indirect or even direct exposure to pesticides (Agüerra 2002).…”

Section: Introductionmentioning

confidence: 99%

Determination of Haloxyfop-Methyl, Linuron, and Procymidone Pesticides in Carrot Using SLE-LTP Extraction and GC-MS

et al. 2015

This study aimed to optimize and validate an analytical method for extraction, detection, and quantification of haloxyfop-methyl, procymidone, and linuron pesticides in carrot samples using solid-liquid extraction methods and low temperature partition (SLE-LTP), accompanied by gas chromatography coupled to mass spectrometry (GC-MS). For SLE-LTP technical optimization, we utilized a complete factorial planning, which had as its variables, agitation time, freezing time, and the correct sample mass/extracting solution volume ratio. The organic extract obtained was analyzed by GC-MS. To test the performance of this procedure, the method was validated and applied to the monitoring of pesticide residues in 20 samples of carrot produced in Alto Paranaíba, Minas Gerais, Brazil. The proposed method showed linearity between 0.5 and 3.5 mg·kg −1 with correlation coefficients greater than 0.99. The quantification limits were 0.48 mg· kg −1 for haloxyfop-methyl, 0.69 mg·kg −1 for linuron, and 0.65 mg·kg −1 for procymidone, values below the maximum residue limit provided by international legislation of 1.0 mg· kg −1 for linuron and procymidone. The use of haloxyfopmethyl is not approved in the cultivation of carrot. The recovery percentages were between 90 and 110 %, with a coefficient of variation of less than 12 %. Ten percent of the carrot samples monitored showed residues of linuron and procymidone in concentrations exceeding those permitted by Brazilian law.