Multiresidue determination of sulfonamides in a variety of biological matrices by supported liquid membrane with high pressure liquid chromatography-electrospray mass spectrometry detection

Msagati, Titus A.M.; Nindi, Mathew Muzi

doi:10.1016/j.talanta.2004.02.038

Cited by 104 publications

(49 citation statements)

References 21 publications

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“…Pecorelli et al (2005) quantificaram dez resíduos de sulfonamidas em amostras de músculo e obtiveram valores de recuperação e seus respectivos coeficientes de variação para STZ, SMZ e SDM de 62,4% (CV = 6,22%), 81,2% (CV = 6,44%) e 75,2% (CV = 5,72%), respectivamente, para a concentração de 100 µg kg -1 . Papapanagiotou et al (2005) verificaram que a porcentagem de recuperação e o coeficiente de variação para SMZ em amostras de leite foram de 76,34 e 9,42%, respectivamente, com limite de quantificação de 50 µg kg -1 . Maudens et al (2004), analisando sufonamidas em amostras de mel por CLAE com derivação pós-coluna, verificaram que os valores de recuperação ficaram entre 37 e 67% com limites de quantificação na faixa de 2 a 5 µg kg -1 .…”

Section: Resultsunclassified

“…As sulfonamidas são fármacos sintéticos utilizados extensivamente para tratamento de infecções bacterianas, causadas por microrganismos gram-positivos e gramnegativos (Pastor-Navarro et al, 2007). Estes compostos são derivados do p-aminobenzenosulfonamida, caracterizados por um grupo arila contendo um grupo amino e um grupo sulfonamida em posição para.…”

Section: Introductionunclassified

“…A separação e a quantificação dos resíduos, por sua vez, são usualmente conduzidas por cromatografia líquida de alta eficiência (CLAE). Diversos detectores têm sido estudados, tais como arranjo de diodos (Hela et al, 2003;Garcia et al, 2004), ultravioleta (Pecorelli et al, 2004), fluorescência (Maudens et al, 2004;Salisbury et al, 2004) e de massas (Dost et al, 2000;Msagati & Nindi, 2004). Na detecção por fluorescência, é necessária a derivação dos analitos.…”

Section: Introductionunclassified

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Sulfonamidas em leite por cromatografia líquida de alta eficiência com derivação pré-coluna e detecção por fluorescência

Alaburda

Ruvieri

Shundo

et al. 2007

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar e validar um método para deteminação de resíduos de sulfatiazol (STZ), sulfametazina (SMZ) e sulfadimetoxina (SDM) em leite UHT integral. A extração foi realizada com diclorometano e coluna de extração em fase sólida de sílica. Os resíduos, após derivação com fluorescamina, foram quantificados por cromatografia líquida de alta eficiência com detector de fluorescência. O limite de detecção das três sulfas em amostra de leite integral foi 0,3 µg L para SDM, com coeficientes de variação entre 4,4 e 6,6%. Os valores de recuperação para STZ, SMZ e SDM foram 63,2, 91,2 e 63,2%, respectivamente. Considerando o limite máximo de resíduo estabelecido pela legislação brasileira de 100 µg kg -1 para a soma das concentrações totais de STZ, SMZ e SDM, o método descrito permite a determinação simultânea dos três analitos em amostras de leite UTH integral.Termos para indexação: fluorescamina, sulfonamida, sulfametazina, sulfadimetoxina. Sulfonamides in milk by high performance liquid chromatography with pre-column derivatization and fluorescence detectionAbstract -The objective of this work was to evaluate and validate a method for analysis of sulfathiazole (STZ), sulfamethazine (SMZ) and sulfadimethoxine (SDM) residues in milk. Extraction was carried out with diclhoromethane followed by silica solid phase extraction. The extracts were derivatizated with fluorescamine and quantified by reversed-phase high performance liquid chromatography with fluorescence detection. The detection limit for the three sulfonamides was 0.3 µg L -1 and the quantification limit was 1 µg L -1 for STZ and SMZ and 2.5 µg L -1 for SDM, with coefficient of variation ranging from 4.4 to 6.6%. The recoveries for STZ, SMZ and SDM were 63.2, 91.2 and 63.2%, respectively. Considering that Brazilian regulation sets maximum residue limit in milk of 100 µg kg -1 for total sulfonamide concentrations (STZ, SMZ and SDM), the present method is adequate to quantify the residues of three sulfonamides simultaneously in UHT milk.

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Section: Resultsunclassified

Section: Introductionunclassified

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Sulfonamidas em leite por cromatografia líquida de alta eficiência com derivação pré-coluna e detecção por fluorescência

Alaburda

Ruvieri

Shundo

et al. 2007

Pesq. agropec. bras.

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“…In recent years, there is an increasing concern about sulfonamide residues in food products since a study demonstrated that these substances are carcinogenic and may reduce the therapeutic effectiveness of drugs on humans. 1 To ensure the safety of food for humans, the European Union (EU) has established maximum residue limit (MRL) of 100 ng g -1 for total sulfonamides in foodstuffs. There is no MRL established in Brazil for eggs yet, so in this work it was adopted 100 ng g -1 for total SAs according to the EU.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of analytical method for sulfonamide residues in eggs by liquid chromatography Tandem Mass Spectrometry based on the Comission Decision 2002/657/EC

Cruz¹,

Soares²,

Marques³

et al. 2011

J. Braz. Chem. Soc.

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A técnica de cromatografia líquida acoplada a um espectrômetro de massas com ionização "electrospray" (LC-ESI-MS/MS) foi usada para a determinação simultânea de resíduos de dez sulfonamidas (SAs: sulfacetamida, sulfatiazol, sulfamerazina, sulfametazina, sulfametoxipiridazina, sulfametazol, sulfacloropiridazina, sulfametoxazol, sulfadimetoxina e sulfaquinoxalina) em ovos. As amostras foram preparadas por precipitação das proteínas com acetonitrila seguida por extração em fase sólida. Após remoção do solvente e reconstituição, os extratos finais foram injetados no LC-ESI-MS/MS no modo positivo para todas as sulfonamidas. O método validado resultou em recuperação entre 87 e 116% com desvios padrão relativo entre 8,5 e 27,2%, CCa entre 101,0 e 122,1 ng g -1 e CCb entre 114,5 e 138,8 ng g -1 . O método analítico, no qual foram utilizadas amostras fortificadas, foi validado baseado na Decisão da Comissão 2002/657/CE.Liquid chromatography-electrospray ionization -tandem mass spectrometry (LC-ESI-MS/MS) was used to simultaneous determination of ten sulfonamide (SAs) residues (sulfacetamide, sulfathiazole, sulfamerazine, sulfamethazine, sulfamethoxypyridazine, sulfamethizole, sulfachloropyridazine, sulfamethoxazole, sulfadimethoxine and sulfaquinoxaline) in eggs. Samples were extracted with acetonitrile and the supernatants were cleaned up by solid phase extraction. After solvent evaporation and reconstitution, aliquots of the final extracts were injected into a LC-ESI-MS/MS in positive mode for all sulfonamides. The validated method resulted in recoveries ranging between 87 and 116% with relative standard deviations between 8.5 and 27.2%, CCa between 101.0 and 122.1 ng g -1 , and CCb between 114.5 and 138.8 ng g -1 . The analytical method, using fortified samples, was validated based on the Commission Decision 2002/657/EC.

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“…The use of SLM for selective extraction of ionizing organic compounds has recently gained popularity. The SLM extraction technique has the capability to handle environmental samples that are highly complicated and biological samples such as sulfonamide in urine and bovine liver and kidney tissue [20], bambuterol (a basic drug) in human plasma [21] or macrolides in bovine kidney and liver tissues [22].…”

Section: Introductionmentioning

confidence: 99%

New supported liquid membrane‐capillary electrophoresis in‐line arrangement for direct selective analysis of complex samples

et al. 2006

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An in-line coupling of a micro-membrane extraction unit, based on supported liquid membrane, with commercially available capillary electrophoresis equipment is described. A main characteristic of this micro-membrane device, made from a simple Eppendorf tube, is that it permits the application of voltage in the acceptor solution to be applied during the extraction process. This has been shown as an alternative to enhance sensitivity, as the analytical signal achieved by applying 10 kV for 20 min was similar to that obtained without the application of voltage and with extraction time of 60 min. In addition, the design has been made permitting both in-line hydrodynamic and electrokinetic sample introduction into the electrophoretic capillary. The analytical potential of the proposed system has been demonstrated by the direct determination of nitroimidazoles from pig liver tissue. The high efficiency of the proposed system allowed the extraction and the determination of the analytes to be performed from a simple tissue homogenate obtained in water. The precision of the analysis of spiked samples, expressed in terms of relative standard deviation, was better than 4.8%.

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Multiresidue determination of sulfonamides in a variety of biological matrices by supported liquid membrane with high pressure liquid chromatography-electrospray mass spectrometry detection

Cited by 104 publications

References 21 publications

Sulfonamidas em leite por cromatografia líquida de alta eficiência com derivação pré-coluna e detecção por fluorescência

Sulfonamidas em leite por cromatografia líquida de alta eficiência com derivação pré-coluna e detecção por fluorescência

Development and validation of analytical method for sulfonamide residues in eggs by liquid chromatography Tandem Mass Spectrometry based on the Comission Decision 2002/657/EC

New supported liquid membrane‐capillary electrophoresis in‐line arrangement for direct selective analysis of complex samples

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