Multipronged quantitative proteomics reveals serum proteome alterations in breast cancer intrinsic subtypes

Gajbhiye, Akshada; Dabhi, Raju; Taunk, Khushman; Jagadeeshaprasad, Mashanipalya G.; RoyChoudhury, Sourav; Mane, Anupama; Bayatigeri, Santhakumari; Chaudhury, Koel; Santra, Manas Kumar; Rapole, Srikanth

doi:10.1016/j.jprot.2017.05.007

Cited by 16 publications

(18 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the analysis of blood-based markers at the subtype level in biological fluids such as plasma and serum allowed finding different protein markers related with the tumor microenvironment and the subtype-specific changes. Following this research line, the proteomic alterations in blood plasma [ 7 ] and blood serum [ 8 ] of BC subtypes were explored.…”

Section: Discussionmentioning

confidence: 99%

“…Classification of BC might be markedly improved if new biomarkers identified with the use of high-throughput “omics” approaches could support diagnosis based on histopathological patterns [ 7 , 8 , 9 ]. Nanomaterials have been introduced into the field of proteomics to establish a new and rapidly evolving research area termed nanoproteomics [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Protein Corona Gold Nanoparticles Fingerprinting Reveals a Profile of Blood Coagulation Proteins in the Serum of HER2-Overexpressing Breast Cancer Patients

Chantada-Vázquez

López

García-Vence

et al. 2020

IJMS

View full text Add to dashboard Cite

Breast cancer (BC) is a molecularly heterogeneous disease that encompasses five major molecular subtypes (luminal A (LA), luminal B HER2 negative (LB-), luminal B HER2 positive (LB+), HER2 positive (HER2+) and triple negative breast cancer (TNBC)). BC treatment mainly depends on the identification of the specific subtype. Despite the correct identification, therapies could fail in some patients. Thus, further insights into the genetic and molecular status of the different BC subtypes could be very useful to improve the response of BC patients to the range of available therapies. In this way, we used gold nanoparticles (AuNPs, 12.96 ± 0.72 nm) as a scavenging tool in combination with Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) to quantitatively analyze the serum proteome alterations in the different breast cancer intrinsic subtypes. The differentially regulated proteins specific of each subtype were further analyzed with the bioinformatic tools STRING and PANTHER to identify the major molecular function, biological processes, cellular origin, protein class and biological pathways altered due to the heterogeneity in proteome of the different BC subtypes. Importantly, a profile of blood coagulation proteins was identified in the serum of HER2-overexpressing BC patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protein Corona Gold Nanoparticles Fingerprinting Reveals a Profile of Blood Coagulation Proteins in the Serum of HER2-Overexpressing Breast Cancer Patients

Chantada-Vázquez

López

García-Vence

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Therefore, multiple high-throughput BC proteomics studies have emerged over the last decade [23][24][25]. However, most studies conducted so far have focused on profiling tumor tissues [26][27][28]or serum samples [29,30]. To the best of our knowledge, only one pilot study conducted by Raso and co-authors [31] applied tandem mass tags (TMT) quantitative mass spectrometry combined with the MudPIT technique to breast TIF samples which were isolated from three patients (two patients with infiltrating ductal carcinomas and one patient with a phylloides tumor) [31].…”

Section: Accepted Articlementioning

confidence: 99%

High‐throughput proteomics of breast cancer interstitial fluid: identification of tumor subtype‐specific serologically relevant biomarkers

et al. 2021

View full text Add to dashboard Cite

Analysis of breast tumor interstitial fluids (TIF) may help identify protein biomarkers that are secreted in the circulation. Following extraction of breast tumor TIF, LC‐MS/MS TMT labeled proteomics, and bioinformatic analyses we identified a panel of secreted protein biomarkers. Experimental and computational validation using tissues and external datasets, respectively, indicated the potential of this biomarker panel for breast cancer subtype stratification.

show abstract

“…A combined isobaric tag for the relative and absolute quantitation (iTRAQ)-liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics approach can simultaneously quantify proteins in 4-or 8-plex samples (Zhou et al 2017). Thus, iTRAQ-LC-MS/ MS is one of the most sensitive proteomics technologies and can detect and quantitatively analyze low-abundance proteins in complex biological samples (Gajbhiye et al 2017;Yan et al 2017;Wang et al 2018). An understanding of immune-related cell proliferation and differentiation in serum at different infection stages will help to reveal the immune pathogenesis of APP infection.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-based quantitative proteomic analysis of peripheral blood serum in piglets infected with Actinobacillus pleuropneumoniae

et al. 2020

View full text Add to dashboard Cite

Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is a swine respiratory disease with an important impact around the world either as a single infection or part of the porcine respiratory disease complex. The data of interaction between hosts and pathogens has becoming more crucial for exploration of the mechanism. However, up to now, comparatively little information is available on the systemic and dynamic changes that occur in pig serum in response to APP infection. This study used iTRAQ to identify differentially expressed proteins (DEPs) in pig serum in response to APP infection. Compared with the APP un-infected group (S0),there were 137 up-regulated and 68 down-regulated proteins at 24 h (S24), and 81 up-regulated and 107 down-regulated proteins at 120 h (S120). At 24 h, the immune response was not significantly enriched, but cell adhesion, cytosol, Golgi apparatus, GTP and ATP binding and regulation of cell cycle were extremely active, implying host preparation of immune response starting. Subsequently, innate immune response, negative regulation of apoptotic process, immunological synapse, adaptive immune response, the regulation of inflammatory response, positive regulation of T cell proliferation were more enhanced at 120 h then that of 24 h, representing innate immunity transferring to the adaptive, while endocytosis, cell adhesion and platelet aggregation showed obvious decline. The pathways of T cell receptor signaling pathway, cytokine-cytokine receptor interaction, complement and coagulation cascades, leukocyte transendothelial migration were active remarkably during all infection period, and more pathways could connect to form innate immune defense networks. Surprisingly, the pathways like amoebiasis, rheumatoid arthritis and malaria had been found upregulated. As a conclusion, APP could delay host inflammatory response to the infection at early stage, and induced innate immunity to convert from adhesion, interaction into complement activation, proteasome digestion, bacterial invasion at later stage. This would increase our understanding of the porcine distinct response to APP infection.

show abstract

Multipronged quantitative proteomics reveals serum proteome alterations in breast cancer intrinsic subtypes

Cited by 16 publications

References 43 publications

Protein Corona Gold Nanoparticles Fingerprinting Reveals a Profile of Blood Coagulation Proteins in the Serum of HER2-Overexpressing Breast Cancer Patients

Protein Corona Gold Nanoparticles Fingerprinting Reveals a Profile of Blood Coagulation Proteins in the Serum of HER2-Overexpressing Breast Cancer Patients

High‐throughput proteomics of breast cancer interstitial fluid: identification of tumor subtype‐specific serologically relevant biomarkers

iTRAQ-based quantitative proteomic analysis of peripheral blood serum in piglets infected with Actinobacillus pleuropneumoniae

Contact Info

Product

Resources

About