Multipotential Mesenchymal Stem Cells from Femoral Bone Marrow Near the Site of Osteonecrosis

Abstract

Stem cell-based therapies for degenerative disorders and injuries are promising in the new era. Multipotential mesenchymal stem cells (MSCs) from bone marrow (BM) are on the leading edge because they are easy to expand in culture while maintaining their multilineage potential. In vitro assessment of the chondrogenic and osteogenic potentials of cultured MSCs has been established, and the BM used in those experiments was exclusively from healthy donors via iliac crest aspiration. It is unknown whether human marrow obtained from femurs also contains these multipotential MSCs. We collected marrow from proximal femurs of two patients undergoing total hip replacement surgery for femoral head osteonecrosis and isolated and culture expanded MSCs to about 20 population doublings. These cells were homogeneously positive for β β1 1-integrin. When pelleted into aggregates and cultured in a medium containing transforming growth factor-β β3 for 14 days, the cells began to express mRNA for aggrecan and collagen type II and to deposit immunoreactive collagen type II and sulfated proteoglycans in the matrix, hallmarks of chondrogenic differentiation. These MSCs could also be differentiated into osteocytic lineage in vitro, as shown by increased expression of alkaline phosphatase activity and deposition of mineral content onto culture plates. These results indicate that femoral BM obtained during hip surgeries also contained multipotential MSCs. These data imply that direct replacement therapy using MSCs from in situ marrow may be possible in the future and that an MSC bank may be established by using marrow from this approach, bypassing the necessity for iliac marrow aspiration from healthy donors. Stem

Fetal bone cells for tissue engineering

“…Recent results have given evidence of aging in marrow stromal progenitor cells [10,[40][41][42][43][44][45][46]. Other reports demonstrated that osteogenic progenitor cells were maintained during aging [47][48][49]. The question of changes in the osteoprogenitor cell population with age is further complicated by the possibility of sex-dependent differences [50][51][52].…”

Housekeeping gene stability influences the quantification of osteogenic markers during stem cell differentiation to the osteogenic lineage

“…Isolation and expansion of human bone marrow mesenchymal stem cells Human bone marrow mesenchymal stem cells were extracted from the trabecular bone in the femoral head of patients after total hip arthroplasty, as described elsewhere with some modifications (Schutze et al 2005;Lee et al 2003;Sakaguchi et al 2004;Quiroz et al 2008). The protocol was approved by the Review Boards of the Hospital Pablo Tobo´n Uribe and the CES University, and all samples were processed after written informed consent was obtained.…”

Accumulated Chromosomal Instability in Murine Bone Marrow Mesenchymal Stem Cells Leads to Malignant Transformation

“…zymed.com). In vitro osteogenic, adipogenic, and chondrogenic assays were done as previously reported [13,14].…”