Fetal bone cells for tissue engineering

Montjovent, Marc-Olivier; Burri, Nathalie; Mark, Silke; Federici, Ermanno; Scaletta, Corinne; Zambelli, Pierre‐Yves; Hohlfeld, P.; Leyvraz, Pierre-François; Applegate, L.; Pioletti, Dominique P.

doi:10.1016/j.bone.2004.07.001

Cited by 77 publications

(84 citation statements)

References 72 publications

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“…Scaffolds developed in this study showed high osteoconductive potential, allowing proliferation of bone cells through the porous structures. Fetal bone cells were already characterized for their high osteogenic potential, 27 and their proliferative aptitude observed on artificial structures is of major interest from the perspective of the use of human primary fetal bone cells in tissue engineering. During the exposure, fetal and adult bone cells seeded on the scaffolds were pushed toward differentiation, and osteoblastic genes such as cbfa-1, ␣ 1 chain of type I collagen, alkaline phosphatase, and osteocalcin were expressed (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…26 The interesting potential of fetal bone cells for tissue engineering was demonstrated on the basis of their rapid growth rate and their responsiveness to differentiation factors. 27 In this study, fetal and adult bone cells were each seeded on the same batches of scaffolds to compare their interactions. We demonstrate here for the first time that once seeded with human fetal bone cells, PLA/ceramic composite osteoconductive scaffoldsprocessed by supercritical gas foaming-give a promising approach in tissue engineering due to the high osteogenic potential of these cells.…”

Section: Introduction Bmentioning

confidence: 99%

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Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

et al. 2005

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The aim of this investigation was to test the biocompatibility of three-dimensional bioresorbable foams made of poly(L-lactic acid) (PLA), alone or filled with hydroxyapatite (HA) or ␤-tricalcium phosphate (␤-TCP), with human primary osteoblasts, using a direct contact method. Porous constructs were processed by supercritical gas foaming, after a melt-extrusion of ceramic/polymer mixture. Three neat polymer foams, with pore sizes of 170, 310, and 600 m, and two composite foams, PLA/5 wt% HA and PLA/5 wt% ␤-TCP, were examined over a 4-week culture period. The targeted application is the bone tissue-engineering field. For this purpose, human fetal and adult bone cells were chosen because of their highly osteogenic potential. The association of fetal bone cells and composite scaffold should lead to in vitro bone formation. The polymer and composite foams supported adhesion and intense proliferation of seeded cells, as revealed by scanning electron microscopy. Cell differentiation toward osteoblasts was demonstrated by alkaline phosphatase (ALP) enzymatic activity, ␥-carboxylated Gla-osteocalcin production, and the onset of mineralization. The addition of HA or ␤-TCP resulted in higher ALP enzymatic activity for fetal bone cells and a stronger production of Gla-osteocalcin for adult bone cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introduction Bmentioning

confidence: 99%

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

et al. 2005

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“…The accepted paradigm in bone tissue engineering is to combine a scaffold with cells and/or growth factors [1][2][3][4][5][6]. The scaffold is used for its osteoconductive properties [7,8] and the cells or growth factors are used for their osteoinductive or osteogenic properties [9,10].…”

Section: Introductionmentioning

confidence: 99%

In vivo loading increases mechanical properties of scaffold by affecting bone formation and bone resorption rates

Roshan-Ghias¹,

Lambers²,

Gholam‐Rezaee³

et al. 2011

Bone

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A successful bone tissue engineering strategy entails producing bone-scaffold constructs with adequate mechanical properties. Apart from the mechanical properties of the scaffold itself, the forming bone inside the scaffold also adds to the strength of the construct. In this study, we investigated the role of in vivo cyclic loading on mechanical properties of a bone scaffold. We implanted PLA/β-TCP scaffolds in the distal femur of six rats, applied external cyclic loading on the right leg, and kept the left leg as a control. We monitored bone formation at 7 time points over 35 weeks using time-lapsed micro-computed tomography (CT) imaging. The images were then used to construct micro-finite element models of bone-scaffold constructs, with which we estimated the stiffness for each sample at all time points. We found that loading increased the stiffness by 60% at 35 weeks. The increase of stiffness was correlated to an increase in bone volume fraction of 18% in the loaded scaffold compared to control scaffold. These changes in volume fraction and related stiffness in the bone scaffold are regulated by two independent processes, bone formation and bone resorption. Using time-lapsed micro-CT imaging and a newly-developed longitudinal image registration technique, we observed that mechanical stimulation increases the bone formation rate during 4-10 weeks, and decreases the bone resorption rate during 9-18 weeks post-operatively. For the first time, we report that in vivo cyclic loading increases mechanical properties of the scaffold by increasing the bone formation rate and decreasing the bone resorption rate.© 2011 Elsevier Inc. All rights reserved. IntroductionThe accepted paradigm in bone tissue engineering is to combine a scaffold with cells and/or growth factors [1][2][3][4][5][6]. The scaffold is used for its osteoconductive properties [7,8] and the cells or growth factors are used for their osteoinductive or osteogenic properties [9,10]. While successful in vivo studies [11,12] and in clinical studies [13], this strategy has difficulties to settle in routine clinical practice. The reasons are manifold but often related to the cost, the stringent regulations established by the regulatory authorities, the specialized infrastructure needed, or the lack of possible reimbursement from health insurances when cells or growth factors are employed.As the use of cells or growth factors is indeed the most difficult element to be included for bone tissue engineering, despite their acknowledged usefulness, we advocate a shift in the bone tissue engineering paradigm. Mechanical loading is an intrinsic stimulation present in the skeleton. It has been demonstrated in numerous in vivo studies to be correlated to bone regulation [14,15]. The in situ mechanical stimulation, if considered in an appropriate way, could then replace the cell and growth factor components of the bone tissue engineering strategy. This new paradigm has been proposed recently [16][17][18]. To support this approach, we have previously shown that controlle...

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“…Fetal bone cells were shown to proliferate, differentiate and finally to mineralize their extra cellular matrix in vitro [1]. For these reasons, we decided to test their association with porous scaffolds for bone repair in vivo.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, fetal bone cells were characterized in vitro to investigate their potential use for tissue engineering [1]. They were shown to be able to proliferate and differentiate into mature osteoblasts in vitro when seeded on PLA/TCP scaffolds [2].…”

Section: Introductionmentioning

confidence: 99%

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Montjovent

Mark

Mathieu

et al. 2008

Bone

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Fetal bone cells were shown to have an interesting potential for therapeutic use in bone tissue engineering due to their rapid growth rate and their ability to differentiate into mature osteoblasts in vitro. We describe hereafter their capability to promote bone repair in vivo when combined with porous scaffolds based on poly(L-lactic acid) (PLA) obtained by supercritical gas foaming and reinforced with 5 wt.% β-tricalcium phosphate (TCP).Bone regeneration was assessed by radiography and histology after implantation of PLA/TCP scaffolds alone, seeded with primary fetal bone cells, or coated with demineralized bone matrix. Craniotomy critical size defects and drill defects in the femoral condyle in rats were employed. In the cranial defects, polymer degradation and cortical bone regeneration were studied up to 12 months postoperatively. Complete bone ingrowth was observed after implantation of PLA/TCP constructs seeded with human fetal bone cells. Further tests were conducted in the trabecular neighborhood of femoral condyles, where scaffolds seeded with fetal bone cells also promoted bone repair.We present here a promising approach for bone tissue engineering using human primary fetal bone cells in combination with porous PLA/TCP structures. Fetal bone cells could be selected regarding osteogenic and immune-related properties, along with their rapid growth, ease of cell banking and associated safety.

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Fetal bone cells for tissue engineering

Cited by 77 publications

References 72 publications

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

In vivo loading increases mechanical properties of scaffold by affecting bone formation and bone resorption rates

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

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