Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential

Abstract

We describe human chorionic mesenchymal stem cell (hCMSC) lines obtained from the chorion of human term placenta with high therapeutic potential in human organ pathology. hCMSCs propagated for more than 100 doublings without a decrease in telomere length and with no telomerase activity. Cells were highly positive for the embryonic stem cell markers OCT-4, NANOG, SSEA-3, and TRA-1– 60. In vitro, cells could be differentiated into neuron-like cells (ectoderm), adipocytes, osteoblasts, endothelial-like cells (mesoderm), and hepatocytes (endoderm)— derivatives of all three germ layers. hCMSCs effectively facilitated repair of injured epithelium as demonstrated in an ex vivo-perfused human lung preparation injured by Escherichia coli endotoxin and in in vitro human lung epithelial cultures. We conclude that the chorion of human term placenta is an abundant source of multipotent stem cells that are promising candidates for cell-based therapies.

Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro

“…Recently, multipotent MSCs have also been isolated in placental tissues, including the amnion and chorion layers of the amniotic membrane,21, 22, 23, 24 and these amniotic membrane derived stem cells have demonstrated the ability to regulate immune cells though secretion of various immunomodulatory cytokines 25, 26, 27, 28…”

Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering

“…Stem cell lines obtained from the chorion of human placenta have been found to be similar to other MSCs, 8,[40][41] such as bone marrow MSCs and adipose MSCs, which could remain viable and reproducible in vitro, could express markers of pluripotent stem cells (NANOG, OCT-4, SSA-3, etc) and surface markers of mesenchymal cells (CD73, CD90, CD105, etc), and could be differentiated in vitro into osteoblasts, adipocytes (mesoderm), neuronlike cells (ectoderm), and hepatocytes (endoderm) with culturing in differential media.…”

“…1, 41 Nazarov et al reported that human chorionic mesenchymal stem cell could remain viable and reproducible for more than 100 doublings (55 passages), which is better than other MSCs. 41 In this study, we successfully prepared PLA/PCEC hybrid membranes by electrostatic spinning technique, and hPMSCs cultured on PLA/PCEC nanofibers showed good cell attachment and robust proliferation and could be induced into osteoblasts when treated with osteogenic differential media, as per a previous study.…”

“…40 Recently, human placenta-derived MSCs (hPMSCs) have been discovered as a new source of stem cells. 40,41 Obtaining the organ is not an invasive procedure, as the placenta is expelled after the birth of the neonate. Cells of the placenta are fetal.…”

“…40 Literature has shown that hPMSCs could remain viable and reproducible during proliferation in vitro and could be differentiated in vitro into neuron-like cells (ectoderm), adipocytes, osteo-blasts, endothelial-like cells (mesoderm), and hepatocytes (endoderm) when cultured in specific induction medium. 8,[41][42][43] In the present study, novel PLA/PCEC hybrid electrospinning fibrous membranes with a nanoporous surface were obtained, and the chemical and physical properties of the PLA/PCEC hybrid scaffolds were investigated by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM), among other methods.…”