Multipotent Adult Progenitor Cells

Verfaillie, Catherine M.; Crabbe, Annelies

doi:10.1016/b978-0-12-374729-7.00028-7

Cited by 2 publications

(2 citation statements)

References 64 publications

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“… 24 – 35 In addition to their broader differentiation potential, MAPC can proliferate without obvious signs of senescence and can be expanded to over 70 passage doublings while remaining cytogenetically normal. 26 , 36 …”

Section: Introductionmentioning

confidence: 99%

“…[24][25][26][27][28][29][30][31][32][33][34][35] In addition to their broader differentiation potential, MAPC can proliferate without obvious signs of senescence and can be expanded to over 70 passage doublings while remaining cytogenetically normal. 26,36 To date, research has focused predominantly on the immunomodulatory properties of MAPC as well as their therapeutic efficacy in models of myocardial infarction and hypoxic ischemia. [37][38][39][40] MAPC have been reported to release proangiogenic factors including VEGF, interleukin 8 (IL-8), and CXCL-5, all of which are required for in vitro vascular tube formation.…”

Section: Introductionmentioning

confidence: 99%

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Multipotent adult progenitor cells on an allograft scaffold facilitate the bone repair process

2016

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Multipotent adult progenitor cells are a recently described population of stem cells derived from the bone marrow stroma. Research has demonstrated the potential of multipotent adult progenitor cells for treating ischemic injury and cardiovascular repair; however, understanding of multipotent adult progenitor cells in orthopedic applications remains limited. In this study, we evaluate the osteogenic and angiogenic capacity of multipotent adult progenitor cells, both in vitro and loaded onto demineralized bone matrix in vivo, with comparison to mesenchymal stem cells, as the current standard. When compared to mesenchymal stem cells, multipotent adult progenitor cells exhibited a more robust angiogenic protein release profile in vitro and developed more extensive vasculature within 2 weeks in vivo. The establishment of this vascular network is critical to the ossification process, as it allows nutrient exchange and provides an influx of osteoprogenitor cells to the wound site. In vitro assays confirmed the multipotency of multipotent adult progenitor cells along mesodermal lineages and demonstrated the enhanced expression of alkaline phosphatase and production of calcium-containing mineral deposits by multipotent adult progenitor cells, necessary precursors for osteogenesis. In combination with a demineralized bone matrix scaffold, multipotent adult progenitor cells demonstrated enhanced revascularization and new bone formation in vivo in an orthotopic defect model when compared to mesenchymal stem cells on demineralized bone matrix or demineralized bone matrix–only control groups. The potent combination of angiogenic and osteogenic properties provided by multipotent adult progenitor cells appears to create a synergistic amplification of the bone healing process. Our results indicate that multipotent adult progenitor cells have the potential to better promote tissue regeneration and healing and to be a functional cell source for use in orthopedic applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multipotent adult progenitor cells on an allograft scaffold facilitate the bone repair process

2016

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Same or Not the Same? Comparison of Adipose Tissue-Derived Versus Bone Marrow-Derived Mesenchymal Stem and Stromal Cells

Strioga

Viswanathan

Darinskas

et al. 2012

Stem Cells and Development

718

558

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Mesenchymal stem/stromal cells (MSCs) comprise a heterogeneous population of cells with multilineage differentiation potential, the ability to modulate oxidative stress, and secrete various cytokines and growth factors that can have immunomodulatory, angiogenic, anti-inflammatory and anti-apoptotic effects. Recent data indicate that these paracrine factors may play a key role in MSC-mediated effects in modulating various acute and chronic pathological conditions. MSCs are found in virtually all organs of the body. Bone marrow-derived MSCs (BM-MSCs) were discovered first, and the bone marrow was considered the main source of MSCs for clinical application. Subsequently, MSCs have been isolated from various other sources with the adipose tissue, serving as one of the alternatives to bone marrow. Adipose tissue-derived MSCs (ASCs) can be more easily isolated; this approach is safer, and also, considerably larger amounts of ASCs can be obtained compared with the bone marrow. ASCs and BM-MSCs share many biological characteristics; however, there are some differences in their immunophenotype, differentiation potential, transcriptome, proteome, and immunomodulatory activity. Some of these differences may represent specific features of BM-MSCs and ASCs, while others are suggestive of the inherent heterogeneity of both BM-MSC and ASC populations. Still other differences may simply be related to different isolation and culture protocols. Most importantly, despite the minor differences between these MSC populations, ASCs seem to be as effective as BM-MSCs in clinical application, and, in some cases, may be better suited than BM-MSCs. In this review, we will examine in detail the ontology, biology, preclinical, and clinical application of BM-MSCs versus ASCs.

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Multipotent Adult Progenitor Cells

Cited by 2 publications

References 64 publications

Multipotent adult progenitor cells on an allograft scaffold facilitate the bone repair process

Multipotent adult progenitor cells on an allograft scaffold facilitate the bone repair process

Same or Not the Same? Comparison of Adipose Tissue-Derived Versus Bone Marrow-Derived Mesenchymal Stem and Stromal Cells

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