Multiplication of Animal Cells in Suspension Measured by Colony Counts

Gwatkin, Ralph B. L.; Till, J. E.; Whitmore, G.F.; Siminovitch, Louis; Graham, Angus

doi:10.1073/pnas.43.6.451

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1958

1966

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Cited by 13 publications

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“…The publication of McLimans et al (1957a), describing the development of the spinner culture system, includes a description of the trypan blue method. Additionally, Gwatkin et al (1957) have demonstrated a good correlation between the total cell count as obtained by a direct counting procedure and actual isolated cell culture using a feeder layer (Puck and Fisher, 1956).…”

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confidence: 99%

The Propagation of Mammalian Cells in a 20-Liter Stainless Steel Fermentor12

Ziegler¹,

Davis²,

Thomas³

et al. 1958

Applied Microbiology

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The propagation of mammalian cells in stainless steel fermentors of a type used in antibiotic fermentations represents a marked departure from the techniques employed in classical tissue culture studies. Since the pioneer work of Harrison (1907), in which he demonstrated the outgrowth of cells from nerve tissue maintained in vitro, many techniques for the in vitro culture of cells have been developed. An older method of cell culture, which is still used, MATERIALS AND METHODS Twenty-L Stainless Steel Fermentor. A diagram of the 20-L stainless steel fermentor5 is given in figure 1. 4New Brtunswick Scientific Co., New Bruinswick, New Jersey.

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confidence: 99%