A new approach to absolute determination of the neutron source yield is presented. It bases on the application of melamine (C 3 H 6 N 6 ) to neutron detection combined with Monte Carlo simulations of neutron transport. Melamine has the ability to detect neutrons via 14 N(n, p) 14 C reaction and subsequent determination of 14 C content. A cross section for this reaction is relatively high for thermal neutrons (1.827 b) and much lower for fast neutrons. A concentration of 14 C nuclei created in the irradiated sample of melamine can be reliably measured with the aid of the accelerator mass spectrometry (AMS). The mass of melamine sufficient for this analysis is only 10 mg. Neutron detection is supported by Monte Carlo simulations of neutron transport carried out with the use of MCNP-4C code. These simulations are aimed at computing the probability of 14 C creation in the melamine sample per the source neutron. The result of AMS measurements together with results of MCNP calculations enable us to determine the number of neutrons emitted from the source during the irradiation of melamine.The proposed method was applied for determining the neutron emission from a commercial 252 Cf neutron source which was independently calibrated. The measured neutron emission agreed with the certified one within uncertainty limits. The relative expanded uncertainty (k=2) of the absolute neutron source yield determination was estimated at 2.6%.Apart from calibration of radionuclide neutron sources the proposed procedure could facilitate absolute yield measurements for more complex sources. Potential applications of this methodology as it is further developed include diagnostics of inertial confinement fusion and plasma-focus experiments, calibration of neutron measurement systems at tokamaks and accelerator-based neutron sources as well as characterization of neutron fields generated in large particle detectors during collisions of hadron beams.