Multiplication and cryopreservation of vanilla (Vanilla planifolia ‘Andrews’)

Gonzalez-Arnao, Maria Teresa; Lazaro-Vallejo, C. E.; Engelmann, Florent; Gámez-Pastrana, R.; Martínez-Ocampo, Yolanda; Pastelín-Solano, Miriam Cristina; Díaz-Ramos, Carlos

doi:10.1007/s11627-009-9242-6

Cited by 51 publications

(19 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The technique involving the fragmentation of shoot apices was used for in vitro propagation of Vitis vinifera, through the formation of multiple adventitious shoots (Barlass and Skene, 1978). Improved multi-plication rates of Vanilla planifolia 'Andrews' was also achieved through fragmentation of the base of shoot clusters (Gonzalez-Arnao et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Micropropagation to Conserve the Endangered Gabal Elba Dragon Tree (Dracaena ombet Heuglin ex Kotschy & Peyr)

Dewir¹,

Aldubai²,

Al-Obeed³

et al. 2019

horts

View full text Add to dashboard Cite

Plant tissue culture offers opportunities for the rescue and conservation of endangered plant species. Here, we report the successful in vitro propagation of Dracaena ombet, an endangered plant. Several physical and chemical seed treatments were evaluated to develop a propagation approach. Germination of D. ombet seeds was monitored for 16 weeks by placing them onto Murashige and Skoog (MS) medium. Maximum seed germination (20%) was recorded when seeds were soaked-scarified, whereas all other treatments did not result in seed germination. Fragmented (longitudinally bisected) and intact in vitro shoots were cultured onto MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) and indole butyric acid (IBA) to induce axillary shoots. Longitudinal fragmentation of explants had a greater effect than the intact explants for shoot proliferation when cultured onto medium containing plant growth regulators. Fragmented shoots cultured onto MS medium supplemented with 2 mg · L L1 BAP and 0.5 mg · L L1 IBA treatment resulted in the highest amount of axillary shoots (seven shoots per explant). The intact shoots had the highest axillary shoots (1.8 shoots per explant) when cultured onto a medium supplemented with a combination of 1 mg · L L1 BAP and 0.5 mg · L L1 IBA. One hundred percent rooting was obtained using half strength MS medium supplemented with 0.5 or 1 mg · L L1 IBA. With full strength MS medium, a maximum rooting of 60% was obtained with 1 mg · L L1 IBA or naphthalene acetic acid (NAA) addition. The plantlets were acclimatized to ex vitro conditions with a 95% survival rate. This study offers a simple method for in vitro propagation of D. ombet, which is valuable to enable conservation of this endangered species.

show abstract

Section: Resultsmentioning

confidence: 99%

Micropropagation to Conserve the Endangered Gabal Elba Dragon Tree (Dracaena ombet Heuglin ex Kotschy & Peyr)

Dewir¹,

Aldubai²,

Al-Obeed³

et al. 2019

horts

View full text Add to dashboard Cite

show abstract

“…Tissues were first exposed to loading solution which is formulated by a single or a combination of chemicals. In terms of concentration viscosity, they are much reduced compared to the vitrification solution [4]. Studies have shown that the mixture of different chemicals and concentration of chemicals in vitrification solution yield different outcomes.…”

Section: Discussionmentioning

confidence: 99%

“…For PVS2 cryopreservation study in roses, in vitro fragmented explants (IFE) represent a new source of explant as starting material. IFEs were first introduced by authors [4] who conducted research on Vanilla planifolia “Andrews”. The study discovered that IFEs allows the production of a larger number of shoots than micro cuttings after 4 months in culture.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation ofRosa hybridacv. Helmut Schmidt by PVS2 vitrification method usingin vitrofragmented explants (IFEs)

Udain

et al. 2019

Preprint

View full text Add to dashboard Cite

1415 This is the first report on cryopreservation via PVS2 vitrification method on roses using in vitro 16 fragmented explants (IFEs) as the starting material. The aim of this study is to optimize the efficient 17 plant recovery and regeneration system for cryopreservation of Rosa hybrida cv. Helmut Schmidt 18 using IFEs. Some important parameters have been optimized in this study are the effect of ascorbic 19 acid (0.3 mM) examined separately and in combination at all steps in cryopreservation procedure 20 (preculture, loading, unloading and growth recovery), loading type, loading duration, and PVS2 21 duration. The highest growth recovery of 43.33% was obtained when 3-4 mm size IFEs precultured 22 on 0.25 M sucrose media supplemented with full-strength MS for one (1) day, followed by loading 23 treatment supplemented with 1.5 M glycerol + 0.4 M sucrose + 5% DMSO + 0.3 mM ascorbic acid 24 for 20 minutes, dehydration with PVS2 solution for 30 minutes and then treated with unloading 25 solution supplemented with 1.2 M sucrose + 0.3 mM ascorbic acid for 20 minutes. This finding 26 implies that long-term storage of Rosa Hybrida cv. Helmut Schmidt by PVS2 vitrification method 27 was successful with essential biomolecules. 28 29 30 Keywords: In vitro fragmented explants (IFEs), PVS2 vitrification, ascorbic acid, Biomolecules, 31 Rose 32 34 Roses are one of the most important ornamental plants in many parts of the world. This 35 is because they bloom beautiful flowers and as such, they are widely used in the horticultural 36 industry. Cultivation of rose plants via tissue culture micropropagation has disadvantages in 37 terms of contamination, somaclonal variations and also due to biotic stresses [1]. Hence, 38 cryogenic storage offers a stable and safe method for long term storage of plant materials 39 while safeguarding genetic stability [2]. Cryopreservation at temperatures of -196°C has been 40 considered as an ideal means for long term storage of plant germplasm. Through 41 cryopreservation, hybrids that are in danger of being extinct can be conserved with low 42 maintenance apart from being easy for international exchange. Cryopreservation through 43 vitrification reduces injury caused by intracellular crystallization and provides high 44 regeneration rates prior to immersion into liquid nitrogen. Basically, the most appropriate 45 plant part utilized is the meristem culture since it generates virus free lines [3]. For PVS2 46 cryopreservation study in roses, in vitro fragmented explants (IFE) represent a new source of 47 explant as starting material. IFEs were first introduced by authors [4] who conducted research 48 on Vanilla planifolia "Andrews". The study discovered that IFEs allows the production of a 49 larger number of shoots than micro cuttings after 4 months in culture. However, every 50 species of plants has different genetic makeup and respond differently to various treatments. 51 Therefore, improvement of the cryopreservation method is crucial in each step for growth 52 after recovery. Vitrification ...

show abstract

“…Circunstancias muy similares son descritas por investigadores mexicanos relacionadas con el desconocimiento real del germoplasma de V. planifolia existente en la región de Totonacapan, Veracruz (Herrera-Cabrera et al, 2012) y otras regiones productoras de México (Castillo y Engleman, 1993). La situación es preocupante cuando muchos autores afirman que V. planifolia es originaria de México (Soto, 1999;Havkin-Frenkel y Belanger, 2007;Castro, 2008;Barrera-Rodríguez et al, 2009;Gonzalez-Arnao et al, 2009;Gigant et al, 2011;Salazar-Rojas et al, 2011;Tapia, 2011;Kelso-Bucio et al, 2012), y que a pesar de contar con gran diversidad genética para hacer fitomejoramiento, el mercado de la vainilla mexicana representa menos de un 1% de la producción mundial (Soto, 2006, Barrera-Rodríguez et al, 2009Salazar-Rojas, 2011;Herrera-Cabrera et al, 2012).…”

Section: Importancia De Vanilla Sppunclassified

Importancia y desafíos de la conservación de Vanilla spp. (Orquidaceae) en Costa Rica.

2014

View full text Add to dashboard Cite

Importance and conservation challenges of Vanilla spp. (Orchidaceae) in Costa Rica. The aim of this work was to describe the importance and the main challenges for the use, management and conservation of the Vanilla genus in Costa Rica. Topics covered in this study were: taxonomy in the country and worldwide, data collection and new estimates of wild relatives, the economic, ecological and social importance, the gene pool of the Costa Rican species, the crop situation worldwide (harvested area, production, yield, major producers), causes that reduce the productive potential of the species, culture methods, the vulnerability of Vanilla planifolia to extinction, and the genetic potential in the country with respect to the concentration of vanillin. The comprehensive conservation strategy proposed in this paper aims to perpetuate vanilla plant genetic resources in gene banks through a formal system of seed production, for further use for breeding, research and commercial production. Through sustainable use, those species will be protected in Costa Rica. The natural or legal persons who use such materials must generate monetary or non-monetary benefits from the use of resources through a fair and equitable system to the suppliers. Agro-ecological conditions of Costa Rica as well as the genetic material of both wild and cultivated vanilla opens the possibility of developing production models based on organic agriculture.

show abstract

Multiplication and cryopreservation of vanilla (Vanilla planifolia ‘Andrews’)

Cited by 51 publications

References 17 publications

Micropropagation to Conserve the Endangered Gabal Elba Dragon Tree (Dracaena ombet Heuglin ex Kotschy & Peyr)

Micropropagation to Conserve the Endangered Gabal Elba Dragon Tree (Dracaena ombet Heuglin ex Kotschy & Peyr)

Cryopreservation ofRosa hybridacv. Helmut Schmidt by PVS2 vitrification method usingin vitrofragmented explants (IFEs)

Importancia y desafíos de la conservación de Vanilla spp. (Orquidaceae) en Costa Rica.

Contact Info

Product

Resources

About