Multiplexed Activity of perAuxidase: DNA-Capped AuNPs Act as Adjustable Peroxidase

Hizir, Mustafa Salih; Top, Meryem; Balcioglu, Mustafa; Rana, Muhit; Robertson, Neil; Shen, Fusheng; Sheng, Jia; Yigit, Mehmet V.

doi:10.1021/acs.analchem.5b03926

Cited by 155 publications

(87 citation statements)

References 48 publications

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“…3(b), black line). To the best of our knowledge, the peak absorbance at 650 nm has been frequently used as a diagnostic peak in previous studies which exploited the peroxidase-mimicking property of AuNPs [30][31][32][33]. However, chemical and physical interferences may have the potential to induce AuNP aggregation, particularly in a medium with high electrolyte content, resulting in a red-shift of the plasmon peak around 650-700 nm.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, studies have focused on the suppression and enhancement of peroxidase-mimicking activity of AuNPs for the detection of H2O2, glucose, DNA and heavy metals. Functional molecules such as aptamers, DNA, antibodies, as well as cystamine and heavy metals have been utilised to tune the enzyme-mimicking activity of AuNPs in the design of biological sensors [30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

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Unusual switchable peroxidase-mimicking nanozyme for the determination of proteolytic biomarker

et al. 2018

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Detection of enzyme biomarkers originating from either bio-fluids or contaminating microorganisms is of utmost importance in clinical diagnostics and food safety. Herein, we present a simple, low-cost and easy-to-use sensing approach based on the switchable peroxidase-mimicking activity of plasmonic gold nanoparticles (AuNPs) that can catalyse for the oxidation of 3,3',5'5-tetramethylbenzidine (TMB) for the determination of protease enzyme. The AuNP surface is modified with casein, showing dual functionalities. The first function of the coating molecule is to suppress the intrinsic peroxidase-mimicking activity of AuNPs by up to 77.1%, due to surface shielding effects. Secondly, casein also functions as recognition sites for the enzyme biomarker. In the presence of protease, the enzyme binds to and catalyses the degradation of the coating layer on the AuNP surface, resulting in the recovery of peroxidase-mimicking activity. This is shown visually in the development of a blue colored product (oxidised TMB) or spectroscopically as an increase in absorbance at 370 and 650 nm. This mechanism allows for the detection of protease at 44 ng•mL −1 in 90 min. The nanosensor circumvents issues associated with current methods of detection in terms of ease of use, compatibility with point-of-care testing, low-cost production and short analysis time. The sensing approach has also been applied for the detection of protease spiked in ultra-heat treated (UHT) milk and synthetic human urine samples at a limit of detection of 490 and 176 ng•mL −1 , respectively, showing great potential in clinical diagnostics, food safety and quality control.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Unusual switchable peroxidase-mimicking nanozyme for the determination of proteolytic biomarker

et al. 2018

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“…Recently, a variety of nanomaterials, such as the nanoparticles of Fe 3 O 4 , 1,2 gold, 3 cerium oxide, 4 carbon-based nanotubes 5 or graphene oxide nanosheets, 6 and the transition-metal dichalcogenides 7 have been observed to possess unique catalytic activities that mimic natural enzymes, termed nanozymes. 8,9 Compared to biological enzymes, nanozymes are more stable, less expensive, and easier to store, with a few showing higher catalytic activity.…”

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confidence: 99%

Enhancement of the Intrinsic Peroxidase-Like Activity of Graphitic Carbon Nitride Nanosheets by ssDNAs and Its Application for Detection of Exosomes

et al. 2017

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The present work investigates the capability of single-stranded DNA (ssDNA) in enhancing the intrinsic peroxidase-like activity of the g-C3N4 nanosheets (NSs). We found that ssDNA adsorbed on g-C3N4 NSs could improve the catalytic activity of the nanosheets. The maximum reaction rate of the H2O2-mediated TMB oxidation catalyzed by the ssDNA-NSs hybrid was at least 4 times faster than that obtained with unmodified NSs. The activity enhancement could be attributed to the strong interaction between TMB and ssDNA mediated by electrostatic attraction and aromatic stacking and by both the length and base composition of the ssDNA. The high catalytic activity of the ssDNA-NSs hybrid permitted sensitive colorimetric detection of exosomes if the aptamer against CD63, a surface marker of exosome, was employed in hybrid construction. The sensor recognized the differential expression of CD63 between the exosomes produced by a breast cancer cell line (MCF-7) and a control cell line (MCF-10A). Moreover, a similar trend was detected in the circulating exosomes isolated from the sera samples collected from breast cancer patients and healthy controls. Our work sheds lights on the possibility of using ssDNA to enhance the peroxidase-like activity of nanomaterials and demonstrates the high potential of the ssDNA-NSs hybrid in clinical diagnosis using liquid biopsy.

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“…When the color of the solution changed from pale yellow to wine red, the AuNPs were formed. The resulting solution was cooled to room temperature and stored in brown glass bottles at 4 °C for future use [27,28,29]. …”

Section: Methodsmentioning

confidence: 99%

High Sensitivity Determination of TNF-α for Early Diagnosis of Neonatal Infections with a Novel and Reusable Electrochemical Sensor

Wang

et al. 2017

Sensors

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Early diagnosis is vital for the reduction of mortality caused by neonatal infections. Since TNF-α can be used as a marker for the early diagnosis, the detection of TNF-α with high sensitivity and specificity has great clinical significance. Herein, a highly sensitive and reusable electrochemical sensor was fabricated. Due to the high specificity of aptamers, TNF-α could be accurately detected from five similar cytokines, even from serum samples. In addition, Au nanoparticles (AuNPs) with a high surface area were able to combine a large number of doxorubicin hydrochloride (DOXh), which made the sensor have a high sensitivity. The sensor had a good linear relationship with TNF-α concentration in the range from 1 to 1 × 104 pg/mL and the lowest detection limit is 0.7 pg/mL. More important was that the sensor could be reused 6 times by a crafty use of chain replacement reaction. Meanwhile, the detection time and cost were greatly reduced. Thus, we believe that these advantages of higher specificity and sensitivity, lower cost, and shorter detection time will provide a stronger potential for early diagnosis of neonatal infections in clinical applications.

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Multiplexed Activity of perAuxidase: DNA-Capped AuNPs Act as Adjustable Peroxidase

Cited by 155 publications

References 48 publications

Unusual switchable peroxidase-mimicking nanozyme for the determination of proteolytic biomarker

Unusual switchable peroxidase-mimicking nanozyme for the determination of proteolytic biomarker

Enhancement of the Intrinsic Peroxidase-Like Activity of Graphitic Carbon Nitride Nanosheets by ssDNAs and Its Application for Detection of Exosomes

High Sensitivity Determination of TNF-α for Early Diagnosis of Neonatal Infections with a Novel and Reusable Electrochemical Sensor

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