Multiplex targeted in vivo cancer detection using sensitive near-infrared SERS nanotags

Maiti, Kaustabh Kumar; Dinish, U. S.; Samanta, Animesh; Vendrell, Marc; Soh, K.L.; Park, Sung-Jin; Olivo, Malini; Chang, Young‐Tae

doi:10.1016/j.nantod.2012.02.008

Cited by 168 publications

(142 citation statements)

References 42 publications

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“…In comparison with non-targeted NPs, it was shown that the targeted probes were sufficiently distributed at the tumour, but also to the liver and spleen, though mostly avoided accumulation in the brain, kidneys, lungs and muscle. Other in vivo studies include multiplex SERS probes for in vivo imaging [341] and using NIRspecific probes [342]. An instrument specifically designed for handling small animals has also been developed for rapid multiplexed SERS imaging using four different Raman reporter particles, with the ability to scan 1 cm 2 in 6 minutes [343].…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

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“…[13] Further in vivo applications have been demonstrated by Maiti et al [103][104][105] In the first of their in vivo experiments nanotags were injected into a mouse model and successfully imaged using SERS. [103] The only difference being that the antibody functionalised nanotags were actively bound to cancerous cells prior to their introduction, highlighting the opportunity to successfully image the nanotags in vivo even after participation in a disease recognition event.…”

Section: In Vivo Imaging and Disease Detectionmentioning

confidence: 99%

“…[104] In the most recent of their work, the group elegantly demonstrated the selectivity of their targeting nanotags. [105] In this study the xenograft was composed of oral squamous cell carcinoma (OSCC) cells which exhibit a differential level of expression of EGFR and HER2. EGFR receptors are present at a much higher level than HER2 receptors on the surface of the OSCC cells.…”

Section: In Vivo Imaging and Disease Detectionmentioning

confidence: 99%

“…EGFR receptors are present at a much higher level than HER2 receptors on the surface of the OSCC cells. [105] In accordance with the expressed receptors three nanotags were injected into the tail of the animal subject, two of which had been functionalised with the EGFR specific antibody and the remaining nanotag was functionalised with the HER2 antibody. [105] The signals which were measured from the site of the xenograft were in accordance with the two nanotags functionalised with the specific antibody and two different reporter molecules.…”

Section: In Vivo Imaging and Disease Detectionmentioning

confidence: 99%

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Surface enhanced Raman spectroscopy (SERS): Potential applications for disease detection and treatment

McAughtrie

Faulds

Graham

2014

Journal of Photochemistry and Photobiology C: Photochemistry Re

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This version is available at https://strathprints.strath.ac.uk/49189/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the +44 (0) Highlights: A range of diseases can be detected by Raman and SERS in vitro, ex vivo and in vivo  In vivo multiplexed detection of cancer was achieved  SERS is also a key step in disease treatment  Studies must be further extended to a physiologically relevant medium and in vivo  The potential exists for the application of the techniques in a clinical setting

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“…2,3 Examples for the successful use of the Raman labeling approach are the discrimination of different cell types, viruses, and other lab-on-a-chip applications. [4][5][6][7][8][9] Also in vivo, these SERS-active structures, such as nanorods or nanospheres, have shown the potential to discriminate these labels inside different tissues. 10,11 Gold nanoparticles have been extensively studied as potential SERS contrast agents for cancer diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

D’Hollander¹,

Mathieu²,

Jans³

et al. 2016

IJN

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The need for sensitive imaging techniques to detect tumor cells is an important issue in cancer diagnosis and therapy. Surface-enhanced Raman scattering (SERS), realized by chemisorption of compounds suitable for Raman spectroscopy onto gold nanoparticles, is a new method for detecting a tumor. As a proof of concept, we studied the use of biocompatible gold nanostars as sensitive SERS contrast agents targeting an ovarian cancer cell line (SKOV3). Due to a high intracellular uptake of gold nanostars after 6 hours of exposure, they could be detected and located with SERS. Using these nanostars for passive targeting after systemic injection in a xenograft mouse model, a detectable signal was measured in the tumor and liver in vivo. These signals were confirmed by ex vivo SERS measurements and darkfield microscopy. In this study, we established SERS nanostars as a highly sensitive contrast agent for tumor detection, which opens the potential for their use as a theranostic agent against cancer.

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Multiplex targeted in vivo cancer detection using sensitive near-infrared SERS nanotags

Cited by 168 publications

References 42 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

Surface enhanced Raman spectroscopy (SERS): Potential applications for disease detection and treatment

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

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