Multiplex reverse transcription PCR Luminex assay for detection and quantitation of viral agents of gastroenteritis

Abstract

Background-Several viruses can cause diarrheal disease, a leading cause of morbidity and mortality worldwide. Existing diagnostic methods include ELISA and nucleic acid amplification, usually performed individually.

High prevalence of noroviruses among hospitalized diarrheal patients in Bangladesh, 2011

“…It was clear from the distribution of different pathogens identified in the study ( Table 1) that the etiology of diarrhea is complex and in a substantial proportion of samples, pathogens were not identified. Development of an enteric pathogen testing panel and discovery of novel pathogens using cutting edge technologies such as MassTag PCR, Taqman array or Luminex assays would be required to explore the true burden of pathogens in greater detail [14,15,16].…”

Sapovirus prevalence in children less than five years of age hospitalised for diarrhoeal disease in South Africa, 2009–2013

“…In these studies, SaVs were common during the winter months and in younger children [1]. Sapovirus prevalence has only been described in a few sub-Saharan African countries, including Kenya (5.7% in <14 years) [6], Tanzania (5.7-6.4% in <5 years) [7,8], Malawi (8% in <5 years) [9], South Africa (SA; 4.1-8.4% in all ages) [10,11], Gabon (9.5% in <5 years) [12] and Burkina Faso (18% in <5 years) [13]. These studies utilized molecular methods for SaV detection with an average prevalence of 7.5% (95% CI 3.4%-11.5%) among diarrhoeal cases.…”

Diverse sapovirus genotypes identified in children hospitalised with gastroenteritis in selected regions of South Africa

“…In Tunisia, SaVs were reported in paediatric outpatients with gastroenteritis and only GI.1 was identified [15]. Sapoviruses have also been reported in patients hospitalised with gastroenteritis in Tanzania [16] and in Malawi [17]. Genotypic characterisation was not conducted in the Tanzanian study [16] and in Malawi, only GII strains were identified [17].…”

“…Sapoviruses have also been reported in patients hospitalised with gastroenteritis in Tanzania [16] and in Malawi [17]. Genotypic characterisation was not conducted in the Tanzanian study [16] and in Malawi, only GII strains were identified [17]. Five different SaV genotypes were identified in HIV-seropositive children in Kenya [18].…”