“…Routine immunization with Hib conjugate vaccines has significantly reduced the incidence of Hib-related diseases in developed countries, although the incidence remains relatively high in underdeveloped regions ( Adegbola et al., 2014 ; Langereis and de Jonge, 2015 ). At present, traditional bacterial culture is the gold standard for the detection of H. influenzae ( Farajzadeh Sheikh et al., 2021 ). However, H. influenzae is a fastidious bacterium that requires special growth factors, long-term culture, and complicated operation procedures, thus the isolation rate remains relatively low, which delays diagnosis and treatment.…”