Multiplex PCR with 16S rRNA Gene-Targeted Primers of
            <i>Bifidobacterium</i>
            spp. To Identify Sources of Fecal Pollution

Bonjoch, Xavier; Blanch, Anicet R.

doi:10.1128/aem.70.5.3171-3175.2004

Cited by 82 publications

(46 citation statements)

References 29 publications

(27 reference statements)

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“…(Shanks et al ., 2007). While Bacteroidales is perhaps the most studied taxonomic group for the development of alternative indicators, a few indicators have also been described for Bifidobacteriaceae (Bonjoch et al ., 2004; Gomez-Donate et al ., 2012), and recently Lachnospiraceae (Newton et al ., 2011). …”

Section: Introductionmentioning

confidence: 99%

Sewage reflects the distribution of human faecal Lachnospiraceae

McLellan

Newton

VandeWalle

et al. 2013

Environmental Microbiology

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Summary Faecal pollution contains a rich and diverse community of bacteria derived from animals and humans, many of which might serve as alternatives to the traditional enterococci and Escherichia coli faecal indicators. We used massively parallel sequencing (MPS) of the 16S rRNA gene to characterize microbial communities from wastewater treatment plant (WWTP) influent sewage from 12 cities geographically distributed across the USA. We examined members of the Clostridiales, which included the families Clostridiaceae, Lachnospiraceae and Ruminococcaceae for their potential as sewage indicators. Lachnospiraceae was one of the most abundant groups of faecal bacteria in sewage, and several Lachnospiraceae high-abundance sewage pyrotags occurred in at least 46 of 48 human faecal samples. Clone libraries targeting Clostridium coccoides (C. coccoides) in sewage samples demonstrated that Lachnospiraceae-annotated V6 pyrotags encompassed the previously reported C. coccoides group. We used oligotyping to profile the genus Blautia within Lachnospiraceae and found oligotypes comprised of 24 entropy components that showed patterns of host specificity. These findings suggest that indicators based on Blautia might have the capacity to discriminate between different faecal pollution sources. Development of source-specific alternative indicators would enhance water quality assessments, which leads to improved ecosystem health and reduced human health risk due to waterborne disease.

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Section: Introductionmentioning

confidence: 99%

Sewage reflects the distribution of human faecal Lachnospiraceae

McLellan

Newton

VandeWalle

et al. 2013

Environmental Microbiology

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“…Certain species, such as B. adolescentis, have been shown to be associated with human feces and have been used as an indicator of human fecal pollution [10, 12, 16]. Recent culture-dependent surveys also suggest the prevalence of Bifidobacter species other than B. adolescentis in feces of commonly reared animals [13].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Four Polymerase Chain Reaction Methods for the Rapid Detection of Human Fecal Pollution in Marine and Inland Waters

Bachoon

Miller

Green

et al. 2010

International Journal of Microbiology

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We compared the effectiveness of three PCR protocols for the detection of Bifidobacterium adolescentis and one PCR protocol for detecting Bacteroidales as indicators of human fecal pollution in environmental samples. Quantitative PCR indicated that a higher concentration of B. adolescentis DNA was recovered from sewage samples on the 0.2 μm filters compared to the 0.45 μm filters, and there was no evidence of qPCR inhibitors in the DNA extracts. With the Matsuki method (1999), B. adolescentis was detected only in undiluted sewage samples. The King method (2007) performed well and detected B. adolescentis in all of the sewage dilutions (from undiluted to 10−4). In contrast, the Bonjoch approach (2004) was effective at detecting B. adolescentis at lower dilutions (10−3) of sewage samples and it gave false positive results with some (3/8) pig fecal samples. Human-specific Bacteroidales (HuBacs) were detected in the lower diluents of sewage samples but was positive in pig (6/8) and cattle fecal samples. PCR detection of B. adolescentis in marine samples from Puerto Rico and freshwater samples from Georgia indicated that the PCR method of King et al. (2007) and the modified Layton method for HuBac were in agreement in detecting human fecal pollution in most sites.

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“…(2015) [1][2][3][4][5][6][7][8][9][10][11][12][13] www.deswater.com doi: 10.1080/19443994.2015.1089192 Membrane fouling involves the adsorption of foulants, through physical and/or chemical processes, present in the feed liquid being transported across the membrane [2]. There has been various membrane fouling processes proposed that include surface fouling, inter-membrane fouling, membrane pore fouling, and cake formation fouling.…”

Section: Desalination and Water Treatmentmentioning

confidence: 99%

“…The main species found in municipal wastewater streams are the fecal bacteria Escherichia coli (E. coli), Enterococci, and fecal coliforms [7,8]. E. coli, among other bacterial species, is a commonly used conventional bacterial indicator for wastewater quality and standards [9].…”

Section: Desalination and Water Treatmentmentioning

confidence: 99%

Characterization, fouling, and performance of synthesized chitosan–glycerol membranes in bacterial removal from municipal wastewater

Cadogan

Lee

Popuri

et al. 2015

Desalination and Water Treatment

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A B S T R A C TThe inherent hydrophilic nature of chitosan has gained significant attention with respect to its application in water treatment processes. This study was aimed to synthesize chitosanglycerol (CSG) membranes for microfiltration (MF) applications in wastewater treatment via the solution casting and solvent evaporation technique. CSG membranes were prepared in the ratios 2:1, 3:1, and 4:1 and were cross-linked with phosphoric acid in the presence of ethanol. The synthesized membranes were characterized by tensile strength, swelling, Fourier transform infrared spectroscopy-attenuated total reflectance and Scanning electron microscopy studies to investigate their structural properties. The porosifier glycerol had a great influence on the tensile strength and elongation than the cross-linker. The pore size distribution of the membranes ranged from 28.1 to 51.6 Å with BET surface areas of 13.8-23.8 m 2 g −1 . Water permeation studies were conducted using a cross-flow MF module in batch analyses. The results indicated that 2:1 CSG membranes effectively removed over 95% of bacteria notably, Escherichia coli from wastewater. Various blocking mechanisms were investigated and are discussed in detail utilizing various fouling models such as standard, intermediate, complete, and cake formation, of which the cake formation model had the highest correlation coefficient of these models.

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Multiplex PCR with 16S rRNA Gene-Targeted Primers of Bifidobacterium spp. To Identify Sources of Fecal Pollution

Cited by 82 publications

References 29 publications

Sewage reflects the distribution of human faecal Lachnospiraceae

Sewage reflects the distribution of human faecal Lachnospiraceae

Comparison of Four Polymerase Chain Reaction Methods for the Rapid Detection of Human Fecal Pollution in Marine and Inland Waters

Characterization, fouling, and performance of synthesized chitosan–glycerol membranes in bacterial removal from municipal wastewater

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