Multiplex PCR system for the rapid diagnosis of respiratory virus infection: systematic review and meta-analysis

Huang, Huai-Shiuan; Tsai, Chi Lin; Chang, Jeffrey S.; Hsu, Tien-Pen; Lin, Samuel; Lee, Chien‐Chang

doi:10.1016/j.cmi.2017.11.018

Cited by 172 publications

(128 citation statements)

References 59 publications

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“…Because 2019-nCoV is a newly discovered virus, the spectrum of the available diagnostic tools is tight. At present, there are several commercially available multiplex NAAT tests for the detection of pathogenic organisms in respiratory specimens in clinical virology laboratories (Beckmann and Hirsch, 2016;Huang et al, 2018, Babady et al, 2018. They can detect HCoV-229E, -NL63, -OC43, and -HKU1.…”

Section: The Studymentioning

confidence: 99%

Novel coronavirus: From discovery to clinical diagnostics

Phan

2020

Infection, Genetics and Evolution

245

220

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Section: The Studymentioning

confidence: 99%

Novel coronavirus: From discovery to clinical diagnostics

Phan

2020

Infection, Genetics and Evolution

245

220

View full text Add to dashboard Cite

“…Technological advances have improved the sensitivity, accessibility, and utility of viral diagnostic tools [16]. Molecular assays have been developed and progressively multiplexed to diagnose a large number of respiratory viruses in a single assay with excellent sensitivity and specificity [10,[17][18][19][20]. The importance of molecular-based diagnostic modalities is currently on the rise, and polymerase chain reaction (PCR) technology is being increasingly used in the clinic to rapidly diagnose respiratory infections [19].…”

Section: Introductionmentioning

confidence: 99%

Increased Detection of Viruses in Children with Respiratory Tract Infection Using PCR

Lin

Hwang

Chiu

et al. 2020

IJERPH

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Respiratory viruses are a common cause of respiratory tract infection (RTI), particularly in neonates and children. Rapid and accurate diagnosis of viral infections could improve clinical outcomes and reduce the use of antibiotics and treatment sessions. Advances in diagnostic technology contribute to the accurate detection of viruses. We performed a multiplex real-time polymerase chain reaction (PCR) to investigate the viral etiology in pediatric patients and compared the detection rates with those determined using traditional antigen tests and virus cultures. Fifteen respiratory viruses were included in our investigation: respiratory syncytial virus A/B (RSV), influenza virus A (FluA) and influenza virus B (FluB), human metapneumovirus (MPV), enterovirus (EV), human parainfluenza virus (PIV) types 1-4, human rhinovirus (RV), human coronavirus OC43, NL63, and 229E, human adenovirus (ADV), and human bocavirus (Boca). In total, 474 specimens were collected and tested. Respiratory viruses were detected more frequently by PCR (357, 75.3%) than they were by traditional tests (229, 49.3%). The leading pathogens were RSV (113, 23.8%), RV (72, 15.2%), PIV3 (53, 11.2%), FluA (51, 10.8%), and ADV (48, 10.1%). For children younger than 5 years, RSV and RV were most prevalent; for children older than 5 years, FluA and ADV were the most frequently detected. Of the specimens, 25.8% (92/357) were coinfected with two or more viruses. RV, Boca, PIV2, FluB, and PIV4 had higher rates of coinfection; MPV and PIV1 had the lowest rates of coinfection (9.1% and 5.3%). To conclude, the detection power of PCR was better than that of traditional antigen tests and virus cultures when considering the detection of respiratory viruses. RSV and RV were the leading viral pathogens identified in the respiratory specimens. One-quarter of the positive specimens were coinfected with two or more viruses. In the future, further application of PCR may contribute to the rapid and accurate diagnosis of respiratory viruses and could improve patient outcomes.

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“…16 Respiratory infections caused by CA-RVs apart from seasonal influenza A/B may have been under-diagnosed before the introduction of multiplex RT-PCR methods. 17,18 As the diagnosis of the precise species of CA-RVI became possible, CA-RVs have had great clinical significance in severely immunocompromised patients. 19 The epidemiology and clinical outcome of adenovirus (AdV), human metapneumovirus (hMPV), parainfluenza (PIV), and RSV in SOT and HSCT recipients have been reported during the past few decades.…”

Section: Introductionmentioning

confidence: 99%