Multiplex PCR identification of the major Pseudomonas aeruginosa serogroups using specific novel target genes

Wang, Chu‐Fang; Ye, Qinghua; Zhang, Jumei; Pang, Rui; Gu, Qihui; Ding, Yu; Wu, Qingping; Wang, Juan

doi:10.1016/j.lwt.2022.113567

Cited by 3 publications

(3 citation statements)

References 35 publications

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“…The heterogeneity in the structure of the Oantigen component of LPS is the primary basis for serotyping schemes, which have evolved from traditional serological methods based on the International Antigenic Typing Scheme (IATS) (Liu et al, 1983;Liu and Wang, 1990) to next-generation sequencingbased approaches (Thrane et al, 2016). Based on the O-antigen gene cluster, P. aeruginosa can be classified into 12 serogroups, and several PCR-based methods have been developed for rapid serogroup identification (Li et al, 2018;Nasrin et al, 2022;Wang et al, 2022). Rapid identification of P. aeruginosa by its serogroup not only allows for targeted treatment approaches, but also mitigates the risk of antibiotic resistance associated with broadspectrum antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Monoclonal antibodies against lipopolysaccharide protect against Pseudomonas aeruginosa challenge in mice

Kang

Mateu-Borrás

Monroe

et al. 2023

Front. Cell. Infect. Microbiol.

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Pseudomonas aeruginosa is a common cause of hospital-acquired infections, including central line-associated bloodstream infections and ventilator-associated pneumonia. Unfortunately, effective control of these infections can be difficult, in part due to the prevalence of multi-drug resistant strains of P. aeruginosa. There remains a need for novel therapeutic interventions against P. aeruginosa, and the use of monoclonal antibodies (mAb) is a promising alternative strategy to current standard of care treatments such as antibiotics. To develop mAbs against P. aeruginosa, we utilized ammonium metavanadate, which induces cell envelope stress responses and upregulates polysaccharide expression. Mice were immunized with P. aeruginosa grown with ammonium metavanadate and we developed two IgG2b mAbs, WVDC-0357 and WVDC-0496, directed against the O-antigen lipopolysaccharide of P. aeruginosa. Functional assays revealed that WVDC-0357 and WVDC-0496 directly reduced the viability of P. aeruginosa and mediated bacterial agglutination. In a lethal sepsis model of infection, prophylactic treatment of mice with WVDC-0357 and WVDC-0496 at doses as low as 15 mg/kg conferred 100% survival against challenge. In both sepsis and acute pneumonia models of infection, treatment with WVDC-0357 and WVDC-0496 significantly reduced bacterial burden and inflammatory cytokine production post-challenge. Furthermore, histopathological examination of the lungs revealed that WVDC-0357 and WVDC-0496 reduced inflammatory cell infiltration. Overall, our results indicate that mAbs directed against lipopolysaccharide are a promising therapy for the treatment and prevention of P. aeruginosa infections.

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Section: Discussionmentioning

confidence: 99%

Monoclonal antibodies against lipopolysaccharide protect against Pseudomonas aeruginosa challenge in mice

Kang

Mateu-Borrás

Monroe

et al. 2023

Front. Cell. Infect. Microbiol.

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“…As previously mentioned, some researchers consider that one of the main limits of mPCR is not being able to discriminate between living and non-viable pathogens. However, other authors view this as an advantage; some multiplex PCR tests correctly identify pathogens even if antimicrobial therapy has already been initiated [62,63].…”

Section: Multiplex Pcr-the Classic Methods and Its Usefulness In Mana...mentioning

confidence: 99%

Trends in Molecular Diagnosis of Nosocomial Pneumonia Classic PCR vs. Point-of-Care PCR: A Narrative Review

et al. 2023

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Nosocomial pneumonia is one of the most frequent hospital-acquired infections. One of the types of nosocomial pneumonia is ventilator-associated pneumonia, which occurs in endotracheally intubated patients in intensive care units (ICU). Ventilator-associated pneumonia may be caused by multidrug-resistant pathogens, which increase the risk of complications due to the difficulty in treating them. Pneumonia is a respiratory disease that requires targeted antimicrobial treatment initiated as early as possible to have a good outcome. For the therapy to be as specific and started sooner, diagnostic methods have evolved rapidly, becoming quicker and simpler to perform. Polymerase chain reaction (PCR) is a rapid diagnostic technique with numerous advantages compared to classic plate culture-based techniques. Researchers continue to improve diagnostic methods; thus, the newest types of PCR can be performed at the bedside, in the ICU, so-called point of care testing—PCR (POC-PCR). The purpose of this review is to highlight the benefits and drawbacks of PCR-based techniques in managing nosocomial pneumonia.

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“…However, standard blood cultures often fail to identify biofilm-embedded pathogens for multiple reasons, and no standardized protocols are in place for their detection [ 52 , 53 ]. Given that the virulence factors of biofilm-forming species have been well characterized [ 54 ], specialized diagnostic approaches—such as molecular techniques—have emerged as promising alternatives for achieving accurate and timely pathogen identification [ 55 , 56 , 57 ].…”

Section: Ds Applicationsmentioning

confidence: 99%

Understanding and Implementing Diagnostic Stewardship: A Guide for Resident Physicians in the Era of Antimicrobial Resistance

Schinas,

Dimopoulos,

Akinosoglou

2023

Microorganisms

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Antimicrobial resistance (AMR) poses a significant global health challenge, exacerbated by the COVID-19 pandemic. Antimicrobial stewardship programs (ASPs) are crucial in managing this crisis, with diagnostic stewardship (DS) emerging as a key component. DS refers to the appropriate use of diagnostic tests to optimize patient outcomes, improve antimicrobial use, and combat multi-drug-resistant (MDR) organisms. Despite its potential, understanding and application of DS remain ambiguous in multiple respects, which, however, do not directly implicate the implementation of such initiatives. DS is particularly important for resident physicians who are often at the forefront of patient care and can significantly influence future AMR strategies. This review provides a comprehensive overview of DS, discussing its importance, potential challenges, and future directions. It emphasizes the need for resident physicians to understand DS principles and integrate them into their clinical practice from the beginning of their careers. The review also highlights the role of various stakeholders in implementing DS and the importance of continuous education and training. Ultimately, DS is not just a clinical tool but a philosophy of care, essential for a more responsive, humane, and effective healthcare system.

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Multiplex PCR identification of the major Pseudomonas aeruginosa serogroups using specific novel target genes

Cited by 3 publications

References 35 publications

Monoclonal antibodies against lipopolysaccharide protect against Pseudomonas aeruginosa challenge in mice

Monoclonal antibodies against lipopolysaccharide protect against Pseudomonas aeruginosa challenge in mice

Trends in Molecular Diagnosis of Nosocomial Pneumonia Classic PCR vs. Point-of-Care PCR: A Narrative Review

Understanding and Implementing Diagnostic Stewardship: A Guide for Resident Physicians in the Era of Antimicrobial Resistance

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