Multiplex PCR for rapid detection of genes encoding acquired metallo- -lactamases

Ellington, Matthew J.; Kistler, James; Livermore, David M.; Woodford, Neil

doi:10.1093/jac/dkl481

Cited by 540 publications

(281 citation statements)

References 4 publications

Supporting

Mentioning

249

Contrasting

Unclassified

Order By: Relevance

“…Enterobacteriaceae colonies growing on the selective plates were also tested by PCR for bla KPC throughout the study period and for bla NDM starting in October 2011 using methods previously described. 19,20 Contacts who tested positive were only classified as cases if the carbapenemase detected by PCR matched that of the index patient, even if the organism differed. For example, if the contact and the index had carbapenemase-producing K. pneumoniae, but one had the bla KPC carbapenemase and the other had bla OXA-48 , the contact was not included as a case, ie, the contact was assumed not to have acquired the CP-CRE from that index.…”

Section: Microbiological Methodsmentioning

confidence: 99%

Risk Factors for Carbapenemase-Producing Carbapenem-Resistant Enterobacteriaceae (CP-CRE) Acquisition Among Contacts of Newly Diagnosed CP-CRE Patients

Schwartz-Neiderman

Braun

Fallach

et al. 2016

Infect. Control Hosp. Epidemiol.

View full text Add to dashboard Cite

objective. Carbapenemase-producing carbapenem-resistant Enterobacteriaceae (CP-CRE) are extremely drug-resistant pathogens. Screening of contacts of newly identified CP-CRE patients is an important step to limit further transmission. We aimed to determine the risk factors for CP-CRE acquisition among patients exposed to a CP-CRE index patient.methods. A matched case-control study was performed in a tertiary care hospital in Israel. The study population was comprised of patients who underwent rectal screening for CP-CRE following close contact with a newly identified CP-CRE index patient. Cases were defined as positive tests for CP-CRE. For each case patient, 2 matched controls were randomly selected from the pool of contacts who tested negative for CP-CRE following exposure to the same index case. Bivariate and multivariate analyses were conducted using conditional logistic regression.results. In total, 53 positive contacts were identified in 40 unique investigations (896 tests performed on 735 contacts) between October 6, 2008, and June 7, 2012. bla KPC was the only carbapenemase identified. In multivariate analysis, risk factors for CP-CRE acquisition among contacts were (1) contact with an index patient for ≥3 days (odds ratio [OR], 9.8; 95% confidence interval [CI], 2.0-48.9), (2) mechanical ventilation (OR, 4.1; 95% CI, 1.4-11.9), and (3) carriage or infection with another multidrug-resistant organism (MDRO; OR, 2.6; 95% CI, 1.0-7.1). Among patients who received antibiotics, cephalosporins were associated with a lower risk of acquisition.conclusions. Patient characteristics (ventilation and carriage of another MDRO) as well as duration of contact are risk factors for CP-CRE acquisition among contacts. The role of cephalosporins requires further study.

show abstract

Section: Microbiological Methodsmentioning

confidence: 99%

Risk Factors for Carbapenemase-Producing Carbapenem-Resistant Enterobacteriaceae (CP-CRE) Acquisition Among Contacts of Newly Diagnosed CP-CRE Patients

Schwartz-Neiderman

Braun

Fallach

et al. 2016

Infect. Control Hosp. Epidemiol.

View full text Add to dashboard Cite

show abstract

“…Multiplex PCR for the bla OXA-23 and the bla OXA-24 genes and simple PCR for bla KPC were carried out on the isolates by using the Gene Amp 9700 PCR System (Applied Biosystems, Singapore). The primers and the cycling conditions for the PCR were as has been described earlier [7][8][9]. PCR for the detection of bla NDM-1 was carried out by using primers, as has been reported previously [10].…”

Section: Screening For the Carbapenemase Productionmentioning

confidence: 99%

Emergence of NDM – 1 in the Clinical Isolates of Pseudomonas aeruginosa in India

Khajuria

Praharaj²,

Kumar³

et al. 2013

JCDR

View full text Add to dashboard Cite

Objective: The present study was undertaken to detect the prevalence of the bla NDM-1 metallo beta lactamases (MBLs) in the isolates of Pseudomonas aeruginosa, which were recovered from various clinical samples from hospitalized patients in a tertiary care centre in Pune, India. Methods:A total of 200 isolates of P. aeruginosa which were obtained from various clinical samples were subjected to antibiotic susceptibility testing by the disc-diffusion method and their MICs were determined by the Vitek -2 Automated Antimicrobial Identification and Susceptibility Testing System against imipenem, meropenem, ticarcillin, amikacin, gentamicin, tobramycin, ciprofloxacin, levofloxacin, moxifloxacin, tigecycline, trimethoprim/sulfamethoxazole, ampicillin/sulbactam, piperacillin/ tazobactam, cefoperazone/sulbactam, cefepime, tetracycline, ceftazidime, ceftriaxone and colistin. Their MICs were also determined by the Etest method against imipenem, meropenem, piperacillin, tobramycin, ceftazidime, tigecycline and colistin. The presence of bla NDM-1 was detected by PCR and it was confirmed by sequencing the gene which was present in the isolates which exhibited carbapenem resistance. The experimental transferability of the plasmids which carried bla NDM-1 was determined by using E. coli J53 as the recipient. Result:In the present study, four isolates of P. aeruginosa, which carried the bla NDM-1 gene, were resistant to imipenem and meropenem. These bla NDM-1 carrying isolates remained susceptible to colistin. The plasmid carrying bla NDM-1 was successfully transferred from the four isolates to E. coli J53 recipients. Conclusions:We are reporting the emergence of the P. aeruginosa carrying NDM-1gene, which exhibited resistance to imipenem and meropenem, for the first time from India.

show abstract

“…Thereafter, isolates were screened for the presence of the carbapenemase genes blaKPC, blaVIM, blaNDM and blaOXA-48 by PCR using primers (custom-synthesized by Microsynth, Balgach, Switzerland) and conditions described previously (Ellington et al, 2007;Poirel et al, 2011). Aeromonas veronii isolates were tested for the imiS gene using primers described earlier (Sánchez-Céspedes et al, 2009).…”

Section: Identification Of Carbapenemase Genesmentioning

confidence: 99%

Occurrence and features of chromosomally encoded carbapenemases in Gram-negative bacteria in farm animals sampled at slaughterhouse level

Zurfluh¹,

Hindermann²,

Nüesch‐Inderbinen³

et al. 2016

SAT

View full text Add to dashboard Cite

Multiplex PCR for rapid detection of genes encoding acquired metallo- -lactamases

Cited by 540 publications

References 4 publications

Risk Factors for Carbapenemase-Producing Carbapenem-Resistant Enterobacteriaceae (CP-CRE) Acquisition Among Contacts of Newly Diagnosed CP-CRE Patients

Risk Factors for Carbapenemase-Producing Carbapenem-Resistant Enterobacteriaceae (CP-CRE) Acquisition Among Contacts of Newly Diagnosed CP-CRE Patients

Emergence of NDM – 1 in the Clinical Isolates of Pseudomonas aeruginosa in India

Occurrence and features of chromosomally encoded carbapenemases in Gram-negative bacteria in farm animals sampled at slaughterhouse level

Contact Info

Product

Resources

About