Emergence of NDM – 1 in the Clinical Isolates of Pseudomonas aeruginosa in India

Abstract

Objective: The present study was undertaken to detect the prevalence of the bla NDM-1 metallo beta lactamases (MBLs) in the isolates of Pseudomonas aeruginosa, which were recovered from various clinical samples from hospitalized patients in a tertiary care centre in Pune, India. Methods:A total of 200 isolates of P. aeruginosa which were obtained from various clinical samples were subjected to antibiotic susceptibility testing by the disc-diffusion method and their MICs were determined by the Vitek -2 Automated Antimicrobial Identification and Susceptibility Testing System against imipenem, meropenem, ticarcillin, amikacin, gentamicin, tobramycin, ciprofloxacin, levofloxacin, moxifloxacin, tigecycline, trimethoprim/sulfamethoxazole, ampicillin/sulbactam, piperacillin/ tazobactam, cefoperazone/sulbactam, cefepime, tetracycline, ceftazidime, ceftriaxone and colistin. Their MICs were also determined by the Etest method against imipenem, meropenem, piperacillin, tobramycin, ceftazidime, tigecycline and colistin. The presence of bla NDM-1 was detected by PCR and it was confirmed by sequencing the gene which was present in the isolates which exhibited carbapenem resistance. The experimental transferability of the plasmids which carried bla NDM-1 was determined by using E. coli J53 as the recipient. Result:In the present study, four isolates of P. aeruginosa, which carried the bla NDM-1 gene, were resistant to imipenem and meropenem. These bla NDM-1 carrying isolates remained susceptible to colistin. The plasmid carrying bla NDM-1 was successfully transferred from the four isolates to E. coli J53 recipients. Conclusions:We are reporting the emergence of the P. aeruginosa carrying NDM-1gene, which exhibited resistance to imipenem and meropenem, for the first time from India.

Colistin-Nonsusceptible Pseudomonas aeruginosa Sequence Type 654 with bla NDM-1 Arrives in North America

“…No reports of E. coli ST522 have been published. P. aeruginosa with bla NDM is relatively uncommon and had previously been described in Serbia (22), France (23), Italy (24), India (25,26), Egypt (27), Denmark (28), and the United Kingdom (29). Moreover, very little is known regarding the molecular epidemiology of P. aeruginosa isolates that contain NDM genes.…”

Molecular characterization of resistance mechanisms in Pseudomonas aeruginosa isolates resistant to carbapenems

“…In addition to VIM, NDM is another leading cause of a high-level of resistance to all carbapenems. P. aeruginosa isolates with blaNDM-1 have been detected also in Serbia [33], France [34], and India [35]. NDM-1 was reported in Egypt with low prevalence among P. aeruginosa isolates where only two out of 33 carbapenem-resistant P. aeruginosa isolates harbor NDM [36].…”

Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacter baumannii : Mechanisms and epidemiology

“…In 2012, a single NDM-1-producing P. aeruginosa belonging to ST235 was isolated in France from a patient previously hospitalised in Serbia [214,215]. More recently, NDM-1-positive P. aeruginosa isolates were recovered in India (four isolates), Italy (a single isolate belonging to ST235), Egypt and Slovakia [216][217][218][219]. Interestingly, the emergence of multidrug-resistant VIM-2-producing P. aeruginosa in Russia is also linked to a ST235-like dominant clone [220].…”