Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacter baumannii : Mechanisms and epidemiology

Potron, Anaïs; Poirel, Laurent; Nordmann, Patrice

doi:10.1016/j.ijantimicag.2015.03.001

Cited by 593 publications

(505 citation statements)

References 319 publications

Supporting

Mentioning

474

Contrasting

Unclassified

Order By: Relevance

“…A detailed resistance mechanisms of antibiotics and their epidemiology in Pseudomonas aeruginosa have recently been reviewed. 112 Emergence of colistin resistance in P. aeruginosa has also been reported worldwide. 113 …”

Section: Klebsiella Pneumoniaementioning

confidence: 99%

“…These b-lactamases confer resistance to extended-spectrum cephalosporins, but inhibited by tazobactam and clavulanic acid. 112 PER-1 producing A. baumannii are prevalent in Turkey, but also disseminated in several Eastern European countries including Russia, Hungary, Romania, East and Southeast Asia including China and Korea and finally detected in the US. 112 TEM, SHV and CTX-M-type ESBLs are rarely found in A. baumannii.…”

Section: Acinetobacter Baumanniimentioning

confidence: 99%

“…67 OXA-51 is a naturally occurring b-lactamase in A. baumannii and normally has weak carbapenemase activity. 112 However, several additionally acquired class D enzymes are described and include OXA-23, ¡40, ¡58, ¡143, and ¡235. These enzymes cause weak resistance to carbapenems, but are not active against extendedspectrum cephalosporins.…”

Section: Acinetobacter Baumanniimentioning

confidence: 99%

“…Thus, high-level resistance usually require other mechanisms involved such efflux and porin loss. 112 OXA-23 enzymes are the most prevalent b-lactamases in A. baumannii and detected worldwide. 117 The ArmA enzyme is the most frequently found methylase which is responsible for high-level resistance to all aminoglycosides in A. baumannii.…”

Section: Acinetobacter Baumanniimentioning

confidence: 99%

See 3 more Smart Citations

Epidemiology of antimicrobial resistance in bloodstream infections

2016

View full text Add to dashboard Cite

Antimicrobial resistance in bacterial pathogens is a worldwide challenge leading high morbidity and mortality in clinical settings. Multidrug resistant patterns in gram-positive and -negative bacteria have resulted in difficult-to-treat or even untreatable infections with conventional antimicrobials. Since the early identification of causative microorganisms and their antimicrobial susceptibility patterns in patients with bacteremia and other serious infections is lacking in many healthcare institutions, broad spectrum antibiotics are liberally and mostly unnecessarily used. Such practice has, in turn, caused dramatic increases in emerging resistance and when coupled with poor practice of infection control, resistant bacteria can easily be disseminated to the other patients and the environment. Thus, availability of updated epidemiological data on antimicrobial resistance in frequently encountered bacterial pathogens will be useful not only for deciding on empirical treatment strategies, but also devising an effective antimicrobial stewardship program in hospitals.

show abstract

Section: Klebsiella Pneumoniaementioning

confidence: 99%

Section: Acinetobacter Baumanniimentioning

confidence: 99%

Section: Acinetobacter Baumanniimentioning

confidence: 99%

Section: Acinetobacter Baumanniimentioning

confidence: 99%

See 2 more Smart Citations

Epidemiology of antimicrobial resistance in bloodstream infections

2016

View full text Add to dashboard Cite

show abstract

“…baumannii is a nosocomial pathogen associated with severe infections in immunocompromised hosts (19). Besides its chromosomally encoded AmpC-type beta lactamase (20), A. baumannii frequently harbors class B and D carbapenemases (21) and extended-spectrum beta-lactamases (ESBL) of the PER, GES, and VEB types (22). RNDtype efflux systems, including AdeABC (23), AdeIJK (24), and AdeFGH (25), further contribute to the multidrug resistance phenotype of clinical isolates.…”

mentioning

confidence: 99%

Structure and Function of the PiuA and PirA Siderophore-Drug Receptors from Pseudomonas aeruginosa and Acinetobacter baumannii

Moynié

Lüscher

Rolo

et al. 2017

Antimicrob Agents Chemother

106

View full text Add to dashboard Cite

The outer membrane of Gram-negative bacteria presents an efficient barrier to the permeation of antimicrobial molecules. One strategy pursued to circumvent this obstacle is to hijack transport systems for essential nutrients, such as iron. BAL30072 and MC-1 are two monobactams conjugated to a dihydroxypyridone siderophore that are active against Pseudomonas aeruginosa and Acinetobacter baumannii. Here, we investigated the mechanism of action of these molecules in A. baumannii. We identified two novel TonB-dependent receptors, termed Ab-PiuA and Ab-PirA, that are required for the antimicrobial activity of both agents. Deletion of either piuA or pirA in A. baumannii resulted in 4-to 8-fold-decreased susceptibility, while their overexpression in the heterologous host P. aeruginosa increased susceptibility to the two siderophore-drug conjugates by 4-to 32-fold. The crystal structures of PiuA and PirA from A. baumannii and their orthologues from P. aeruginosa were determined. The structures revealed similar architectures; however, structural differences between PirA and PiuA point to potential differences between their cognate siderophore ligands. Spontaneous mutants, selected upon exposure to BAL30072, harbored frameshift mutations in either the ExbD3 or the TonB3 protein of A. baumannii, forming the cytoplasmic-membrane complex providing the energy for the siderophore translocation process. The results of this study provide insight for the rational design of novel siderophore-drug conjugates against problematic Gramnegative pathogens.

show abstract