Multiplex PCR assay for simultaneous detection of six major bacterial pathogens of rice

Cui, Zhipeng; Ojaghian, Mohammad Reza; Zheng, Tao; Kakar, Kaleem Ullah; Zeng, Jianbin; Zhao, Wenran; Duan, Y. P.; Cruz, C. M. Vera; Li, Bin; Zhu, Bo; Xie, Guishui

doi:10.1111/jam.13094

Cited by 33 publications

(21 citation statements)

References 44 publications

(38 reference statements)

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“…Soil microorganisms are essential to the environment because they function in many reactions, such as the formation of soil structure, cycling of mineral compounds, decomposition of organic materials, promotion/suppression of plant growth, and various soil biological‐physico‐chemical processes . They play crucial roles in biogeochemical cycling and ecosystem functioning, thereby influencing soil fertility and ecosystem stability directly . Here, we investigated the effect of melatonin on both bacterial and fungal populations sampled from the rhizosphere and found distinct differences in their composition between replant soil and healthy soil that were further amplified when melatonin was supplemented.…”

Section: Discussionmentioning

confidence: 98%

The mitigation effects of exogenous melatonin on replant disease in apple

Zhao

Gao

et al. 2018

Journal of Pineal Research

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Melatonin mediates many physiological processes in plants. The problem of apple replant disease is unsolved. Our study objectives were to evaluate the regulatory effect of melatonin on plant resistance to this challenge and investigate the preliminary mechanism by which melatonin helps alleviate the effects of this disease. Two-year-old trees of "Fuji" apple (Malus domestica), grafted onto rootstock M.26, were grown in "replant" soil for 6 months in the absence or presence of a 200 μmol/L melatonin supplement. The addition of melatonin to the soil significantly increased the rates of plant growth and net photosynthesis and chlorophyll concentrations under replant conditions. This molecule elevated the levels of K in leaves and roots and enhanced the activity of soil enzymes. Such supplementation also changed the composition of the bacterial and fungal communities in the soil. We concluded that the application of melatonin to a replant soil can protect their chloroplasts from oxidative damage and release the apple root from membrane damage, and also lead to increased soil enzyme activity and soil quality while altering the composition of bacterial and fungal communities. These changes can then promote seedling growth, stimulate photosynthesis, and elevate K levels, thereby alleviating the effects of apple replant disease.

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Section: Discussionmentioning

confidence: 98%

The mitigation effects of exogenous melatonin on replant disease in apple

Zhao

Gao

et al. 2018

Journal of Pineal Research

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“…; Cui et al . ). The contaminated seeds are the most important primary source of inocula and a means of dissemination of the pathogen for outbreak of the disease to new plants (Shakya et al .…”

Section: Introductionmentioning

confidence: 97%

“…avenae) attacks many crops including rice, maize, oat, sorghum, millet and sugarcane (Hayward 1960;Song et al 2004;Ibrahim et al 2012). In rice, this pathogen causes bacterial brown stripe disease (BBS) with massive economic losses in many ricegrowing countries Cui et al 2016). The contaminated seeds are the most important primary source of inocula and a means of dissemination of the pathogen for outbreak of the disease to new plants (Shakya et al 1985;Kadota et al 1991;Tian et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae

et al. 2018

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“…Particularly, this well-known seed-borne bacterium [ 1 , 2 ] causes bacterial brown stripe (BBS) in rice, which leads to heavy economic losses. It has been reported in many rice-growing countries such as Asia, Africa, North America and Europe [ 3 , 4 ]. The contaminated seeds represent the most important primary source of inocula [ 2 , 5 , 6 ] for outbreak of the disease.…”

Section: Introductionmentioning

confidence: 99%

Role of the Genes of Type VI Secretion System in Virulence of Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp. avenae Strain RS-2

Masum

Yang

et al. 2017

IJMS

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The Type VI secretion system (T6SS) is a class of macromolecular machine that is required for the virulence of gram-negative bacteria. However, it is still not clear what the role of T6SS in the virulence of rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae (Aaa) is. The aim of the current study was to investigate the contribution of T6SS in Aaa strain RS2 virulence using insertional deletion mutation and complementation approaches. This strain produced weak virulence but contains a complete T6SS gene cluster based on a genome-wide analysis. Here we compared the virulence-related phenotypes between the wild-type (RS-2) and 25 T6SS mutants, which were constructed using homologous recombination methods. The mutation of 15 T6SS genes significantly reduced bacterial virulence and the secretion of Hcp protein. Additionally, the complemented 7 mutations ΔpppA, ΔclpB, Δhcp, ΔdotU, ΔicmF, ΔimpJ, and ΔimpM caused similar virulence characteristics as RS-2. Moreover, the mutant ΔpppA, ΔclpB, ΔicmF, ΔimpJ and ΔimpM genes caused by a 38.3~56.4% reduction in biofilm formation while the mutants ΔpppA, ΔclpB, ΔicmF and Δhcp resulted in a 37.5~44.6% reduction in motility. All together, these results demonstrate that T6SS play vital roles in the virulence of strain RS-2, which may be partially attributed to the reductions in Hcp secretion, biofilm formation and motility. However, differences in virulence between strain RS-1 and RS-2 suggest that other factors may also be involved in the virulence of Aaa.

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Multiplex PCR assay for simultaneous detection of six major bacterial pathogens of rice

Cited by 33 publications

References 44 publications

The mitigation effects of exogenous melatonin on replant disease in apple

The mitigation effects of exogenous melatonin on replant disease in apple

Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae

Role of the Genes of Type VI Secretion System in Virulence of Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp. avenae Strain RS-2

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