“…In addition, an NE outbreak was observed only in this trial. A high abundance of C. perfringens in the chicken intestine favors NE development ( Si et al, 2007 ; Moore, 2016 ), and NetB is the major virulence factor involved ( Mostafa et al, 2016 ; Prescott et al, 2016 ; Gharib-Naseri et al, 2021 ). Therefore, a limited number of birds were sampled in Trial 2 for the analyses of C. perfringens population and, netB gene abundance, and the expression of different stress-related genes in the host tissues.…”