Multiplex PCB-based electrochemical detection of cancer biomarkers using MLPA-barcode approach

Sánchez, Josep Lluís Acero; Henry, Olivier; Joda, Hamdi; Solnestam, Beata Werne; Kvastad, Linda; Johansson, E. Susanne; Akan, Pelin; Lundeberg, Joakim; Lladach, N.; Ramakrishnan, Dheeraj; Riley, Ian; O’Sullivan, Ciara K.

doi:10.1016/j.bios.2016.04.018

Cited by 40 publications

(54 citation statements)

References 23 publications

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“…Detection is enabled through the use of horse-radish peroxidase (HRP) labelled secondary probes that are complementary to universal primer sequence. Besides cost reduction and high specificity reported for this assay, sensitivity limit was as low as 25pM [73]. The high success rate for MLPA in multiplex SNP genotyping led to the development of several other MLPA versions for other applications.…”

Section: Multiplex Ligation Dependent Probe Amplification (Mlpa)mentioning

confidence: 94%

“…MLPA is the most widely used LCR based technique for SNP genotyping [68][69][70][71][72][73]130,132,157,160]. It is very simple, robust, highly sensitive, specific, fully automated with multiplexing potential and high-throughput applications.…”

Section: Comparative Evaluation For Various Versions Of Lcr and Ligatmentioning

confidence: 99%

“…It is very simple, robust, highly sensitive, specific, fully automated with multiplexing potential and high-throughput applications. It can detect up to 50 different SNPs at a time with a detection limit of as low as 25pM [68,73]. It has been widely used for various genotyping platforms.…”

Section: Comparative Evaluation For Various Versions Of Lcr and Ligatmentioning

confidence: 99%

“…The only drawback for this powerful technique is high incurred cost with the use of either a genetic analyzer or a microarray scanner. This could be overcome through the use of fabricated electrode array [73]. MOL-PCR is another widely used LCR technique that is similar to MLPA but with flow cytometric detection that enabled better sensitivity and faster analysis [74][75][76].…”

Section: Comparative Evaluation For Various Versions Of Lcr and Ligatmentioning

confidence: 99%

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Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

Gibriel

Adel

2017

Mutation Research/Reviews in Mutation Research

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Genetic variants have been reported to cause several genetic diseases. Various genotyping assays have been developed for diagnostic and screening purposes but with certain limitations in sensitivity, specificity, cost effectiveness and/or time savings. Since the discovery of ligase chain reaction (LCR) in the late nineties, it became one of the most favored platforms for detecting these variants and also for genotyping low abundant contaminants. Recent and powerful modifications with the integration of various detection strategies such as electrochemical and magnetic biosensors, nanoparticles (NPs), quantum dots, quartz crystal and leaky surface acoustic surface biosensors, DNAzyme, rolling circle amplification (RCA), strand displacement amplification (SDA), surface enhanced raman scattering (SERS), chemiluminescence and fluorescence resonance energy transfer have been introduced to both LCR and ligation based amplifications to enable high-throughput and inexpensive multiplex genotyping with improved robustness, simplicity, sensitivity and specificity. In this article, classical and up to date modifications in LCR and ligation based amplifications are critically evaluated and compared with emphasis on points of strength and weakness, sensitivity, cost, running time, equipment needed, applications and multiplexing potential. Versatile genotyping applications such as genetic diseases detection, bacterial and viral pathogens detection are also detailed. Ligation based gold NPs biosensor, ligation based RCA and ligation mediated SDA assays enhanced detection limit tremendously with a discrimination power approaching 1.5aM, 2aM and 0.1fM respectively. MLPA (multiplexed ligation dependent probe amplification) and SNPlex assays have been commercialized for multiplex detection of at least 48 SNPs at a time. MOL-PCR (multiplex oligonucleotide ligation) has high-throughput capability with multiplex detection of 50 SNPs/well in a 96 well plate. Ligase detection reaction (LDR) is one of the most widely used LCR versions that have been successfully integrated with several detection strategies with improved sensitivity down to 0.4fM.

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Section: Multiplex Ligation Dependent Probe Amplification (Mlpa)mentioning

confidence: 94%

Section: Comparative Evaluation For Various Versions Of Lcr and Ligatmentioning

confidence: 99%

Section: Comparative Evaluation For Various Versions Of Lcr and Ligatmentioning

confidence: 99%

Section: Comparative Evaluation For Various Versions Of Lcr and Ligatmentioning

confidence: 99%

See 2 more Smart Citations

Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

Gibriel

Adel

2017

Mutation Research/Reviews in Mutation Research

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“…Messenger RNAs (mRNAs) are a class of single strand and the emerging class of sensitive biomarker for disease diagnosis and prognosis. Most of electrochemical approaches have been developed for mRNA detection are based on hybridization of a target sequence with a surface bound receptor probe and the use of enzymes for signal amplification [179]. Among sensing methods, electrochemical DNA biosensor Reproduced from [166] with permission from Elsevier.…”

Section: Electrochemical Genosensor For Detection Of Rnasmentioning

confidence: 99%

Ultrasensitive Bioaffinity Electrochemical Sensors: Advances and New Perspectives

Alizadeh

Salimi

2018

Electroanalysis

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This article reviews recent advances and developments in the field of bioaffinity electrochemical sensors with emphasis on a subclass including immunosensors, aptasensors, genosensors and molecularly imprinted sensosr. Recent insights into novel fabrication methodologies and electrochemical techniques have resulted in the demonstration of biosensors able to detection of wide range of target analyte. It has been shown that due to their high specificity and sensitivity, they represent a great tool in practical applications. Furthermore, at last couple decades nanotechnology has become very popular in the sensor fields due to the high capacity of nanomaterials to immobilize of bioaffinity agents and also their inhibiting effects on denaturation of enzymes and other bioaffinity systems, which they could well have beneficial effects for the performance of these sensors. The main focus of this review is to discuss methods for immobilizing bioreceptors onto a solid support, introduction of nanomaterials for improve sensitivity of biosensor and various electrochemical sensing platforms. The use of biosensor in these applications is most appropriate, because the analysis of trace substances in medical and healthcare applications, environmental science, and food industries is so important. In addition, recent advances in nanomaterial‐mediated bioaffinity sensors used for onset biosensing, point‐of‐care testing and healthcare management are also highlighted.

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Innovative Quantification of Critical Quality Attributes

Papamatthaiou

Moschou

2023

Advances in Experimental Medicine and Biology

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Multiplex PCB-based electrochemical detection of cancer biomarkers using MLPA-barcode approach

Cited by 40 publications

References 23 publications

Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

Ultrasensitive Bioaffinity Electrochemical Sensors: Advances and New Perspectives

Innovative Quantification of Critical Quality Attributes

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