Multiplex Lateral Flow Immunoassay for Mycotoxin Determination

Abstract

A new lateral flow immunoassay (LFA) is proposed for qualitative and/or semiquantitative determination of aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON), and their analogues (AFs, ZEAs, DONs) in cereal samples. Each of the mycotoxin specific antibody was class specific and there was no cross reactivity to other groups of compounds. The visual limits of detection (vLOD) of the strip were 0.03, 1.6, and 10 μg/kg for AFB1, ZEA and DON, respectively. The calculated limits of detection (cLOD) were 0.05, 1, and 3 μg/kg, respectively. Meanwhile the cutoff values were achieved at 1, 50, and 60 μg/kg for AFB1, ZEA and DON, respectively. Recoveries ranged from 80% to 122% and RSD from 5% to 20%. Both the vLOD and cLOD for the three mycotoxins were lower than the EU maximum levels. Analysis of naturally contaminated maize samples resulted in a good agreement between the multiplex LFA and LC-MS/MS (100% for DONs and AFs, and 81% for ZEAs). Careful analysis of the results further explained the general overestimation of LFA compared to chromatographic methods for quantification of mycotoxins.

Near Infrared Spectrometry for Rapid Non-Invasive Modelling of Aspergillus-Contaminated Maturing Kernels of Maize (Zea mays L.)

“…Rapid and early detection is important for prompt intervention. Current detection methods include high-performance liquid chromatography (HPLC), Enzyme Linked Immunosorbent Assay ELISA, fluorometric assays and lateral flow assays [16][17][18][19]. However, these methods are invasive, require specialised skills and are weak for real time assessments.…”

A Review on Lateral Flow Test Strip for Food Safety

“…Kolosova et al (2007) developed a LFTS for simultaneous detection of two mycotoxins in wheat, deoxynivalenol (DON) and zearalenone (ZEA). Song et al (2014) detected three mycotoxins simultaneously with a multianalyte LFTS. The LFTSs could detect AFB1, ZEA, and DON in cereal samples with result almost similar to those of the LC-MS/MS analysis.…”

Precision Target Guide Strategy for Applying SERS into Environmental Monitoring

“…By using the separation ability of paper-based strip, the paper-based substrate is possibly applied for trace detection from the complex sample containing multiple components. Lateral flow assay (LFA) strip biosensor has been extensively used in point-of-care (POC) test, infectious disease diagnosis and field detection for hazardous materials in environmental samples [71]. In a typical detection procedure, the mobile phase is first pulled through the stationary phase capillary action, then passes through capture zone, and the labeled probes are then captured and detected.…”