Multiplex Cytokine Analyses in Ear Canals of Dogs Suggest Involvement of IL-8 Chemokine in Atopic Otitis and Otodectic Mange—Preliminary Results

Lecru, Line-Alice; Combarros, Daniel; Moog, Fabien; Marinovic, Lukrecija; Kondratjeva, Jevgenija; Amalric, Nicolas; Pressanti, Charline; Cadiergues, Marie‐Christine

doi:10.3390/ani12050575

Cited by 3 publications

(8 citation statements)

References 38 publications

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“…However, in this study, no significant increases in these cytokines were observed in the dogs with ceruminous Malassezia OE; specifically, IL-6 was barely detected in these samples. A previous study using the Luminex ® assay demonstrated that IL-6 was undetectable in the ear canal of dogs with atopic OE (6). In contrast, this study showed significantly increased concentrations of IL-1ß and IL-6 in the cerumen of dogs with suppurative bacterial OE.…”

Section: Discussioncontrasting

confidence: 70%

“…In an analysis of cytokine expression in conjunctival swabs, IL-8 was the only cytokine that was significantly increased in atopic dogs, showing a positive correlation with conjunctival and pruritus scores (7). In addition, the concentration of IL-8 is also significantly higher in the ears of atopic dogs than in healthy dogs (6). Interestingly, in contrast to the present study, the previous study only selected non-infected otitis patients to avoid the production of pro-inflammatory cytokines related to microbes.…”

Section: Discussionmentioning

confidence: 95%

“…Therefore, research on non-invasive methods for analyzing skin cytokine expression has recently become of interest. These methods employ collecting samples from the cerumen, ocular surface, and stratum corneum by tape stripping (3,(5)(6)(7). These non-invasive methods allow sampling from many patients and analysis of the expression of cytokines in the skin.…”

Section: Introductionmentioning

confidence: 99%

“…A previous study investigating miRNA expression in dogs with otitis externa (OE) demonstrated miRNA profile changes between healthy and otitis-affected dogs, and bio-informatics showed that altered miRNAs in the cerumen may be involved in the modulation of the host immune response (5). In addition, a previous study using multiplex cytokine analyses in the ear canals of dogs with OE caused by atopic dermatitis showed increased levels of interleukin (IL)-8 and IL10 (6). However, the previous study selected only non-infected OE to avoid the production of pro-inflammatory cytokines related to microorganism contamination/proliferation.…”

Section: Introductionmentioning

confidence: 99%

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Non-invasive evaluation of cytokine expression using the cerumen of dogs with otitis externa

Yoon,

Park

2024

Front. Vet. Sci.

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The development of a non-invasive method to analyze cytokine expression in the skin will provide further understanding of inflammatory skin disorders. This study aimed to evaluate cytokine expression in the skin through cerumen swabbing in dogs with otitis externa (OE) and to investigate whether increased cytokine expression in infected OE reflects the inflammatory status of the ear canal. Three groups consisting of control dogs (n = 24), dogs with ceruminous Malassezia OE (n = 25), and dogs with suppurative bacterial OE (n = 15) were included in the study. The concentrations of keratinocyte-derived cytokines including Interleukin (IL)-8/chemokine ligand (CXCL)8, IL-10, IL-6, Tumor necrosis factor (TNF)-α, and IL-1ß in the cerumen of the ear canal of the included patients were analyzed using commercial ELISA kits. Additionally, correlations between cytokine levels and cytology scores (of Malassezia yeasts, cocci/rod-shaped bacteria, and inflammatory cells) were assessed. IL-8/CXCL8 concentrations were significantly higher in dogs with ceruminous Malassezia OE and dogs with suppurative bacterial OE than in control dogs. Furthermore, IL-8/CXCL8 concentrations positively correlated with Malassezia scores in dogs with ceruminous OE (r = 0.630) and with bacterial scores in dogs with suppurative OE (r = 0.601). In addition, increased expression of IL-6 and IL-1ß were detected in dogs with suppurative bacterial OE compared to those with Malassezia OE and control dogs, and showed positive correlation with inflammatory cell scores IL-6 r = 0.520, IL-1ß; r = 0.680). Therefore, keratinocyte-derived cytokines could be evaluated using non-invasive methods such as cerumen swabbing in dogs with OE.

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Section: Discussioncontrasting

confidence: 70%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Non-invasive evaluation of cytokine expression using the cerumen of dogs with otitis externa

Yoon,

Park

2024

Front. Vet. Sci.

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“…A study conducted in 2022 demonstrated the presence of inflammatory biomarkers in atopic otitis in dogs, particularly the chemokine IL-8 [27]. Unlike on the skin, inflammatory biomarkers have never been identified in the ear canals of atopic dogs.…”

Section: Variablementioning

confidence: 99%

External Otitis Associated with Atopy Disease in Dogs: A Case-Control Study

Samakkhah¹,

Iraee²,

Peysokhan³

et al. 2022

Act Sci VetSci

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Skin is an organ of living beings afflicted with various disorders such as atopic disease. Atopic disease results from an excess of immunoglobulin E produced due to excessive exposure to non-harmful environmental allergens that affect the skin of various organs, including the ear, leading to external otitis. During this case-control study, 50 dogs referred to clinics in Mazandaran province in Iran with symptoms of atopic otitis were the case group, and 50 dogs with healthy ears were considered the control group. The diagnosis was derived from the clinical findings of the case group, which included itchy skin, redness in the pubic and abdominal area, and ear swelling. The results indicated that 66% of dogs with atopic otitis were in the chronic stage of the disease. Furthermore, there is 4.63 times greater odds of wax secretions in cases of atopic otitis. Additionally, in the case of atopic otitis, the odds of odorous secretions is 5.1 times greater than that of odorless secretions. Lastly, most dogs with this condition had significantly dark ear secretions. Atopic otitis, therefore, is characterized by waxy, odorous and dark secretions in the ears of animals. In general, identifying the causative agent for this disease in dogs is extremely important and can prevent the application of costly and blind treatments. We conducted the first epidemiological study in Iran of external otitis associated with atopy in dogs. These results support a better understanding of the disease.

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Clinical and Microbiological Performances and Effects on Lipid and Cytokine Production of a Ceruminolytic Ear Cleaner in Canine Erythemato-Ceruminous Otitis Externa

Moog

Mivielle

Brun

et al. 2022

Veterinary Sciences

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Erythemato-ceruminous otitis externa (ECOE) is the most common type of otitis in dogs and is generally associated with bacterial and/or yeast infections. The performance of an ear cleaner was assessed over two weeks in canine ECOE, associated with a mild or moderate secondary infection, in a prospective open-label study. Forty ear canals with ECOE that did not receive any type of aural treatment and were not cleaned for 7 days were included. Pruritus (PS), 0–3 Otitis Index Score (OTIS-3) and 0–4 scale cytology (CYTO) scores were assessed on Day (D) 0, D7 and D14. Concentrations of a panel of 13 cytokines on the ear canal surface and the lipid profile of the exudate were measured on D0 and D14. From D0 to D12 or D13, the dogs’ ears were cleaned daily if the secretion score (SEC) was 3/3, every second day if the score was 2/3 and every third day if the score was 1/3. PS, OTIS-3, SEC and CYTO were significantly lower on D7 compared to baseline (−40%, −31%, −36%, −34%, respectively; p < 0.0001). The same parameters decreased further on D14 (−60%, −53%, −61%, −73%, respectively; p < 0.0001) and amounts of interleukin 8 and chemokine KC-like were also reduced compared to baseline (−45%, p < 0.01; −36%, p = 0.3, respectively). The lipid profile was also modified, with a decrease in free lipids and an increase in bound lipids.

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Multiplex Cytokine Analyses in Ear Canals of Dogs Suggest Involvement of IL-8 Chemokine in Atopic Otitis and Otodectic Mange—Preliminary Results

Cited by 3 publications

References 38 publications

Non-invasive evaluation of cytokine expression using the cerumen of dogs with otitis externa

Non-invasive evaluation of cytokine expression using the cerumen of dogs with otitis externa

External Otitis Associated with Atopy Disease in Dogs: A Case-Control Study

Clinical and Microbiological Performances and Effects on Lipid and Cytokine Production of a Ceruminolytic Ear Cleaner in Canine Erythemato-Ceruminous Otitis Externa

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