Multiplex amplification refractory mutation system PCR (ARMS-PCR) provides convenient method for differentiation of canine parvovirus vaccine and field strains

Gupta, Vikas; Chander, Vikas; Chakravarti, Soumendu; Sharma, Gaurav Kumar; Malla, Javed Ahmed; Gupta, Vivek Kumar

doi:10.1007/s13337-018-0491-z

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…No samples were collected from dogs with a history of vaccination in 20 days before sample collection. Vaccine strains might be shed in the post-vaccination period [22][23][24].…”

Section: Methodsmentioning

confidence: 99%

First report of canine parvovirus molecular detection in Bangladesh

Akter

Chowdhury

2021

Vet World

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Background and Aim: Canine parvovirus (CPV) is the most important cause of mortality in dogs in many parts of the world. Clinical cases exhibit characteristic signs, including foul-smelling bloody diarrhea, vomiting, fever, and dehydration. This study assessed field and vaccine variants of parvovirus in the Chattogram metropolitan area, Bangladesh. The investigation also aimed to identify risk factors for this disease. This research is the first to identify the presence of CPV in Bangladesh through molecular examination. Materials and Methods: From October to December 2019, a total of 100 dogs were included in the study. Rectal swabs were taken from all dogs. Twenty dogs showed clinical signs of parvovirus. All clinically affected animals along with 20 randomly selected healthy dogs were tested using amplification refractory mutation system (ARMS)-polymerase chain reaction (PCR) to identify variants from the samples. Logistic regression model analysis was performed to determine the possible risk factors for CPV. Results: ARMS-PCR showed the presence of all three variants, CPV2a, CPV2b, and CPV2c, in clinically ill dogs, and vaccines available in the study area showed either CPV2a or CPV2b strain. The CPV2c variants showed a higher incidence than the other variants. All apparently healthy animals tested were molecularly negative. Multivariable logistic regression model (generalized linear mixed model) indicated that exotic breeds were 3.83 times more likely to be infected by CPV than local breeds. Furthermore, dogs reared in semi-intensive and extensive management systems were 3.64 and 3.79 times more likely to be infected, respectively, than those reared in an intensive management system. Conclusion: These findings provide practitioners and pet owners information on the occurrence of different variants and help design effective prevention strategies for CPV infection.

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“…No samples were collected from dogs with a history of vaccination in 20 days before sample collection. Vaccine strains might be shed in the post-vaccination period [22][23][24].…”

Section: Methodsmentioning

confidence: 99%

First report of canine parvovirus molecular detection in Bangladesh

Akter

Chowdhury

2021

Vet World

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“…This approach is closer to be achieved if the exponential growing of the genetic databases is considered mainly if it is also taken into consideration that those animals under a chronic or persistent infection can excrete infectious viral particles for a lifetime period [ 166 , 167 ]. Thus, the use of highly sensitive molecular techniques has, nowadays, given rise to an increase in vaccine virus detections and differentiation [ 153 , 154 , 168 ]. In previous years, several research groups have developed some molecular biology-based CSF genetic DIVA systems.…”

Section: Discriminatory Tool For Diva Vaccinementioning

confidence: 99%

A Critical Review about Different Vaccines against Classical Swine Fever Virus and Their Repercussions in Endemic Regions

et al. 2021

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Classical swine fever (CSF) is, without any doubt, one of the most devasting viral infectious diseases affecting the members of Suidae family, which causes a severe impact on the global economy. The reemergence of CSF virus (CSFV) in several countries in America, Asia, and sporadic outbreaks in Europe, sheds light about the serious concern that a potential global reemergence of this disease represents. The negative aspects related with the application of mass stamping out policies, including elevated costs and ethical issues, point out vaccination as the main control measure against future outbreaks. Hence, it is imperative for the scientific community to continue with the active investigations for more effective vaccines against CSFV. The current review pursues to gather all the available information about the vaccines in use or under developing stages against CSFV. From the perspective concerning the evolutionary viral process, this review also discusses the current problematic in CSF-endemic countries.

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“…These methods were developed for use with high throughput techniques but costs and practicability have so far limited the wider application of these technologies. The tetra-primer amplification refractory mutation system PCR (tetra-primer ARMS-PCR) developed by Ye et al has proved to be an economical and effective method for SNP genotyping [25][26][27] . Positioning the two outer primers at different distances from the polymorphic nucleotide, causes the two allele-specific amplicons differ in length, allowing them to be discriminated by gel.…”

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confidence: 99%

Establishment and application of a novel method based on single nucleotide polymorphism analysis for detecting β-globin gene cluster deletions

Zhan

Wang

et al. 2020

Sci Rep

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β-Globin gene mutations reduce or terminate the production of beta globin chains, of which approximately 10% are large deletions within the β-globin gene cluster. Because gene deletion leads to loss of heterozygosity at single nucleotide polymorphism (SNP), a novel method for detecting β-globin gene cluster deletions based on SNP heterozygosity analysis was established in this study. The location range of SNPs was selected according to the breakpoint of β-globin gene cluster deletions. SNPs were screened using bioinformatics analysis and population sequencing data. A novel method which enables genotyping of multiplex SNPs based on tetra-primer ARMS-PCR was designed and optimized. Forty clinical samples were tested in parallel by this method and MLPA to verify the performance of this method for detecting β-globin gene cluster deletion. Six informative SNPs were obtained, achieving heterozygote coverage of 93.3% in normal individuals. Genotyping of six SNPs were successfully integrated into two multiplex tetra-primer ARMS-PCR reactions. The sensitivity, specificity, positive predictive value and negative predictive value of the method for detecting β-globin gene cluster deletion were 100%, 96.30%, 92.86%, and 100%, respectively. This is a simple, cost-effective and novel method for detecting β-globin gene cluster deletions, which may be suitable for use in combination with MLPA for thalassemia molecular testing.

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Multiplex amplification refractory mutation system PCR (ARMS-PCR) provides convenient method for differentiation of canine parvovirus vaccine and field strains

Cited by 4 publications

References 10 publications

First report of canine parvovirus molecular detection in Bangladesh

First report of canine parvovirus molecular detection in Bangladesh

A Critical Review about Different Vaccines against Classical Swine Fever Virus and Their Repercussions in Endemic Regions

Establishment and application of a novel method based on single nucleotide polymorphism analysis for detecting β-globin gene cluster deletions

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