Multiple vitellogenins from the Haemaphysalis longicornis tick are crucial for ovarian development

Boldbaatar, Damdinsuren; Umemiya‐Shirafuji, Rika; Liao, Min; Tanaka, Tetsuya; Xuan, Xuenan; Fujisaki, Kozo

doi:10.1016/j.jinsphys.2010.05.019

Cited by 98 publications

(86 citation statements)

References 51 publications

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“…No effect on HlVg-2 was observed from silencing of either Hlfer1 or Hlfer2. Due to the marked effect of Hlfer1 silencing on HlVg-1, we further examined its expression in the midgut, where it was reported to be mainly expressed (Boldbaatar et al, 2010b). Nevertheless, we only found reduction in expression after Hlfer1 knockdown, in contrast with the result using whole tick cDNA (supplementary material Fig.S4C).…”

Section: Fig2 Rt-pcr (A) and Western Blot (B) Analyses In Differentmentioning

confidence: 72%

“…The full-length cDNA library of H. longicornis was constructed using the vector-capping method (Kato et al, 2005), and expressed sequence tags (EST) were prepared in our laboratory (Boldbaatar et al, 2010b). cDNA clones encoding ferritin were identified and selected from the EST database for further analysis.…”

Section: Identification and Characterization Of Hlfer Cdna Clonesmentioning

confidence: 99%

“…Three vitellogenin genes (HlVg-1, HlVg-2 and HlVg-3) have been identified from H. longicornis and have been shown to be essential for the tick's reproduction (Boldbaatar et al, 2010b). Interestingly, we found that silencing of Hlfer1 led to silencing of HlVg-1 and reduced the expression of HlVg-3, whereas (A)Protein expression in the midgut and salivary glands from unfed (Uf), 4-day partially fed (Pf) and engorged (En) adult ticks and hemolymph including hemocytes from partially fed adult ticks.…”

Section: Impact Of Hlfer Silencing On Feeding Survival and Reproductionmentioning

confidence: 99%

“…In addition, the expression of two vitellogenin genes, HlVg-1 and HlVg-3, was affected by silencing of ferritin genes. Boldbaatar et al (Boldbaatar et al, 2010b) showed that multiple vitellogenins are essential for oocyte development and oviposition in H. longicornis. Vitellogenin production was previously shown to be dependent on the degree of feeding (Rosell-Davis and Coons, 1989), and surpassing critical mass is important in vitellogenesis in ixodid ticks (Friesen and Kaufman, 2009).…”

Section: Table1 Effects Of Hlfer1 and Hlfer2 Silencing On Blood Feementioning

confidence: 99%

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Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis

Galay

Aung

Umemiya‐Shirafuji

et al. 2013

Journal of Experimental Biology

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SUMMARYTicks are obligate hematophagous parasites and important vectors of diseases. The large amount of blood they consume contains great quantities of iron, an essential but also toxic element. The function of ferritin, an iron storage protein, and iron metabolism in ticks need to be further elucidated. Here, we investigated the function a newly identified secreted ferritin from the hard tick Haemaphysalis longicornis (HlFER2), together with the previously identified intracellular ferritin (HlFER1). Recombinant ferritins, expressed in Escherichia coli, were used for anti-serum preparation and were also assayed for iron-binding activity. RT-PCR and western blot analyses of different organs and developmental stages of the tick during blood feeding were performed. The localization of ferritins in different organs was demonstrated through an indirect immunofluorescent antibody test. RNA interference (RNAi) was performed to evaluate the importance of ferritin in blood feeding and reproduction of ticks. The midgut was also examined after RNAi using light and transmission electron microscopy. RT-PCR showed differences in gene expression in some organs and developmental stages. Interestingly, only HlFER2 was detected in the ovary during oviposition and in the egg despite the low mRNA transcript. RNAi induced a reduction in post-blood meal body weight, high mortality and decreased fecundity. The expression of vitellogenin genes was affected by silencing of ferritin. Abnormalities in digestive cells, including disrupted microvilli, and alteration of digestive activity were also observed. Taken altogether, our results show that the iron storage and protective functions of ferritin are crucial to successful blood feeding and reproduction of H. longicornis. Supplementary material available online at

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Section: Fig2 Rt-pcr (A) and Western Blot (B) Analyses In Differentmentioning

confidence: 72%

Section: Identification and Characterization Of Hlfer Cdna Clonesmentioning

confidence: 99%

Section: Impact Of Hlfer Silencing On Feeding Survival and Reproductionmentioning

confidence: 99%

Section: Table1 Effects Of Hlfer1 and Hlfer2 Silencing On Blood Feementioning

confidence: 99%

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Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis

Galay

Aung

Umemiya‐Shirafuji

et al. 2013

Journal of Experimental Biology

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“…Vitellogenesis, the synthesis and oocyte deposition of the yolk protein precursor (vitellogenin), is a key process for ovarian development and oocyte maturation induced by blood meal in ticks [152]. Three genes encoding vitellogenin have been identified and characterized in H. longicornis [102]. Silencing these genes through RNAi greatly reduced the reproductive capacity of female ticks, which showed immature and light-colored oocytes.…”

Section: Genes Related To Reproductive Functionmentioning

confidence: 99%

RNA Interference – A Powerful Functional Analysis Tool for Studying Tick Biology and its Control

Galay¹,

Umemiya‐Shirafuji²,

MasamiMochizuki³

et al. 2016

RNA Interference

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Ticks (Acari: Ixodida) are blood-sucking arthropods globally recognized as vectors of numerous diseases. They are primarily responsible for the transmission of various pathogens, including viruses, rickettsiae, and blood parasites of animals. Ticks are second to mosquitoes in terms of disease transmission to humans. The continuous emergence of tick-borne diseases and acaricide resistance of ticks necessitates the development of new and more effective control agents and strategies; therefore, understanding of different aspects of tick biology and their interaction with pathogens is very crucial in developing effective control strategies. RNA interference (RNAi) has been widely used in the area of tick research as a versatile reverse genetic tool to elucidate the functions of various tick proteins. During the past decade, numerous studies on ticks utilized RNAi to evaluate potentially key tick proteins involved in blood feeding, reproduction, evasion of host immune response, interaction with pathogens, and pathogen transmission that may be targeted for tick and pathogen control. This chapter reviewed the application of RNAi in tick research over the past decade, focusing on the impact of this technique in the advancement of knowledge on tick and pathogen biology.

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Vitellogenesis in spiders: first analysis of protein changes in different reproductive stages of Polybetes pythagoricus

et al. 2019

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Multiple vitellogenins from the Haemaphysalis longicornis tick are crucial for ovarian development

Cited by 98 publications

References 51 publications

Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis

Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis

RNA Interference – A Powerful Functional Analysis Tool for Studying Tick Biology and its Control

Vitellogenesis in spiders: first analysis of protein changes in different reproductive stages of Polybetes pythagoricus

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