Vitellogenesis in spiders: first analysis of protein changes in different reproductive stages of Polybetes pythagoricus

Romero, Sofia; Laino, Aldana; Arrighetti, Florencia; Garcı́a, Carlos; Cunningham, M.

doi:10.1007/s00360-019-01217-9

Cited by 7 publications

(2 citation statements)

References 125 publications

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“…The antibody recognized subunits of 70 and 42kDa, from very early embryo stages, as previously seen in P. saltans (Trabalon et al 2017). The presence of subunits of molecular weights similar to those described in the present work has been reported in the vitellogenic ovary and eggs of the spider Polybetes pythagoricus (Romero et al 2019) and Tegenaria atrica (Pourié and Trabalon 2003). Bednarek et al (2019) showed a link between the gene of vitellogenin of the spider Parasteatoda tepidariorum with the expression of the subunits of 47 kDa, confirming that these small subunits are not a product of the degradation of bigger subunits, but form part of the lipovitellins.…”

Section: Contributions Of Lipovitellin From Residual Vitellussupporting

confidence: 89%

Residual vitellus and energetic state of wolf spiderlings Pardosa saltans after emergence from egg-sac until first predation

et al. 2020

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The aim of this study was to evaluate energetic source used by juveniles of a terrestrial oviparous invertebrate during the earliest periods of their life. Growth, behavioural activities and energy contents of Pardosa saltans spiderlings' residual vitellus were monitored during 8 days after their emergence from their egg-sac until they disperse autonomously. The life-cycle of juvenile after emergence can be divided into three periods: a gregarious while juveniles are aggregated on their mother, dismounting off their mother's back and dispersion. We present the first biochemical study of residual vitellus and energy expenditure during these three periods. At emergence, the mean weight of juveniles was 0.59 mg and energy stock from residual vitellus averaged 51 cal/g wet mass. During gregarious period, the weight of the juveniles aggregated on their mother did not vary significantly and juveniles utilized only 1 cal/day from their residual vitellus. During the period from dismounting until their first exogenous feed, juveniles lost weight and used 30 % of their residual vitellus stock. Proteins from the residual vitellus contributed principally to their energy expenditure during this period: 1.5 µg protein/day. Juveniles' first exogenous feeding was observed 7-8 days after emergence, when 70 % of residual vitellus energy had been utilized. Juveniles dispersed after eating, reconstituting an energy stock comparable to that observed at emergence from egg-sac (50 cal/g wet mass). This new energy stock contains mainly lipids unlike the energy stock from the residual vitellus.

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Section: Contributions Of Lipovitellin From Residual Vitellussupporting

confidence: 89%

Residual vitellus and energetic state of wolf spiderlings Pardosa saltans after emergence from egg-sac until first predation

et al. 2020

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“…Consequently, H. graminicola (Group HL mean: 26% dry mass) matured with much higher lipid than P. pseudoannulata (Group HL mean: 19% dry mass). Second, as protein are also important for reproduction ( Barry and Wilder 2013 ; Romero et al 2019 ; Montoro et al 2021 ), a much higher protein demand for growth and too high lipid reserve at maturation may limit the amount of protein that H. graminicola could consume and further invest in egg production. Meanwhile, protein is not a big problem for egg production of female P. pseudoannulata with a much larger body to store more protein.…”

Section: Discussionmentioning

confidence: 99%

The effects of prey lipid on female mating and reproduction of a wolf spider

Feng

Wen

et al. 2022

Current Zoology

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As predators, the macronutrients spiders extract from their prey play important roles in their mating and reproduction. Previous studies of macronutrients on spider mating and reproduction focus on protein, the potential impact of prey lipid content on spider mating and reproduction remains largely unexplored. Here we tested the influence of prey varying in lipid content on female mating, sexual cannibalism, reproduction and offspring fitness in the wolf spider Pardosa pseudoannulata. We acquired two groups of fruit fly Drosophila melanogaster that differed significantly in lipid but not protein content by supplementing cultural media with a high or low dose of sucrose on which the fruit flies were reared (HL: high lipid; LL: low lipid). Subadult (i.e., one molt before adult) female spiders that fed HL flies matured with significantly higher lipid content than those fed LL flies. We found that the mated females fed with HL flies significantly shortened pre-oviposition time and resulted in a significantly higher fecundity. However, there was no significant difference in female spiders varying in lipid content on other behaviors and traits, including the latency to courtship, courtship duration, mating, copulation duration, sexual cannibalism, offspring body size and survival. Hence, our results suggest that the lipid content of prey may be a limiting factor for female reproduction, but not for other behavioral traits in the wolf spiders P. pseudoannulata.

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Transport pathways of hydrocarbon and free fatty acids to the cuticle in arthropods and hypothetical models in spiders

Trabalon

Garcı́a

2021

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Vitellogenesis in spiders: first analysis of protein changes in different reproductive stages of Polybetes pythagoricus

Cited by 7 publications

References 125 publications

Residual vitellus and energetic state of wolf spiderlings Pardosa saltans after emergence from egg-sac until first predation

Residual vitellus and energetic state of wolf spiderlings Pardosa saltans after emergence from egg-sac until first predation

The effects of prey lipid on female mating and reproduction of a wolf spider

Transport pathways of hydrocarbon and free fatty acids to the cuticle in arthropods and hypothetical models in spiders

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