Multiple‐Unit Activity Recording of the Gonadotropin‐Releasing Hormone Pulse Generator

Okamura, Hiroaki; Mori, Yuji

doi:10.1002/9781118606803.ch14

Cited by 2 publications

(1 citation statement)

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“…2 ) and the projection patterns of gC2-ir fibers ( Fig. 3 ) were essentially comparable with those containing kisspeptin- or NKB-ir cells and fibers in other species [ 3 , 4 , 21 , 35 , 38 , 39 ] and to some extent in goats [ 40 , 41 ], albeit with slight species differences. Because amino acid sequences of the 12 C-terminal residues of kisspeptin are identical in goats, cattle, sheep, pigs, rats, and hamsters, the gC2 serum can be employed in studies that use those animals.…”

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confidence: 66%

Mapping of KNDy neurons and immunohistochemical analysis of the interaction between KNDy and substance P neural systems in goat

Okamura

Yamamura

Wakabayashi

2017

Journal of Reproduction and Development

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A population of neurons in the arcuate nucleus (ARC) coexpresses kisspeptin, neurokinin B (NKB), and dynorphin, and therefore they are referred to as KNDy neurons. It has been suggested that KNDy neurons participate in several brain functions, including the control of reproduction. The present study aimed to advance our understanding of the anatomy of the KNDy neural system. We first produced an antiserum against goat kisspeptin. After confirming its specificity, the antiserum was used to histochemically detect kisspeptin-positive signals. Using the colocalization of kisspeptin and NKB immunoreactivity as a marker for KNDy neurons, we mapped distributions of their cell somata and fibers in the whole brain (except the cerebellum) of ovariectomized (OVX) goats. KNDy neuronal somata were distributed throughout the ARC, and were particularly abundant in its caudal aspect. KNDy neuronal fibers projected into several areas within the septo-preoptic-hypothalamic continuum, such as the ARC, median eminence, medial preoptic nucleus, and bed nucleus of the stria terminalis. Kisspeptin immunoreactivity was not found outside of the continuum. We then addressed to the hypothesis that substance P (SP) is also involved in the KNDy neural system. Double-labeling immunohistochemistry for kisspeptin and SP revealed that KNDy neurons did not coexpress SP, but nearly all of the KNDy neuronal somata were surrounded by fibers containing SP in the OVX goats. The present results demonstrate anatomical evidence for a robust association between the KNDy and SP neural systems.

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