Multiple response optimization of a liquid chromatographic method for determination of fluoroquinolone and nitroimidazole antimicrobials in serum and urine

Mannemala, Sai Sandeep; Valliappan, K.

doi:10.1016/j.clinbiochem.2015.12.013

Cited by 11 publications

(3 citation statements)

References 27 publications

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“…Currently, the methods of monitoring NOR focus on instrumental analytical methods and immunoassays (Annunziata et al, 2016;Bucknall, Silverlight, Coldham, Thorne, & Jackman, 2003;Chierentin & Salgado, 2016;Huang et al, 2016;Jiang et al, 2013;Liu et al, 2015;Mannemala & Kannappan, 2016;Mendes et al, 2015;Tong, Liu, Xie, & Li, 2016;Zhao et al, 2014). The most commonly used instrumental analytical methods including high-performance liquid chromatography and liquid chromatography-mass spectrometry are able to detect multiple fluoroquinolones, including NOR, simultaneously to give both quantitative and qualitative results, but the requirements for expensive apparatus and complicated sample preparation limit their application when monitoring samples in bulk (Tao et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Development of an icELISA and immunochromatographic strip for detection of norfloxacin and its analogs in milk

Peng¹,

Liu²,

Kuang³

et al. 2016

Food and Agricultural Immunology

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We developed a monoclonal antibody (mAb) to detect norfloxacin (NOR) using a novel hapten by introducing an amino group via amido linkage into the piperazine ring of NOR. The sensitivity of an indirect competitive enzyme-linked immunosorbent assay (icELISA) based on this mAb showed an improved IC 50 value from 1.18 to 0.12 ng/mL and the limit of detection from 0.37 to 0.05 ng/mL by using a heterologous coating (pazufloxacin coupled to ovalbumin). The recovery rate for NOR in milk ranged from 77.3% to 117.8%. Cross-reactivity (CR) experiments showed good CR with nine common fluoroquinolone analogs: pefloxacin, lomfloxacin, ciprofloxacin, enrofloxacin, dafloxacin, sparfloxacin, ofloxacin, marbofloxacin and flumequine. Furthermore, based on this antigen-antibody combination, an immunochromatographic strip with cut-off value of 1 ng/mL was developed. Given their good sensitivity and CR, the developed icELISA and immunochromatographic strip could be useful for detection of NOR and its analogs in milk.

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Section: Introductionmentioning

confidence: 99%

Development of an icELISA and immunochromatographic strip for detection of norfloxacin and its analogs in milk

Peng¹,

Liu²,

Kuang³

et al. 2016

Food and Agricultural Immunology

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“…Numerous high-performance liquid chromatography (HPLC) methods with fluorescence (FL) detection (Bardot, Vest, Renard, & Renaudeau, 2005;De Smet et al, 2009;Hemanth Kumar & Ramachandran, 2009;Muchohi et al, 2011;Neckel et al, 2002;Sousa, Alves, Campos, Fortuna, & Falcão, 2013;Watabe et al, 2010) and liquid chromatography-tandem mass spectrometry methods (LC-MS/ MS) (Ji et al, 2006;Pranger, Alffenaar, Wessels, Greijdanus, & Uges, 2010;Zander et al, 2015) have been published for the quantification of the selected FQs in human plasma or serum. However, only a few HPLC methods with ultraviolet (UV) detection have been described for the simultaneous quantification two or more FQs (Cavazos-Rocha, Carmona-Alvarado, Vera-Cabrera, Waksman- de-Torres, & Salazar-Cavazos, 2014;Helmy, 2013;Khan et al, 2016;Locatelli et al, 2015;Locatelli, Cifelli, Carlucci, & Romagnoli, 2016;Mannemala & Kannappan, 2016;Srinivas, Narasu, Shankar, & Mullangi, 2008). To our best knowledge, no HPLC-UV method has been published for the simultaneous quantification of LEV, PEF, CIP and MOX in human plasma or serum.…”

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confidence: 99%

Simultaneous quantification of levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin in microvolumes of human plasma using high‐performance liquid chromatography with ultraviolet detection

Zheng

Wang

Lui

et al. 2019

Biomedical Chromatography

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Levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin are four fluoroquinolones used in the treatment of serious bacterial infections. The antibacterial activity of fluoroquinolones is concentration dependent. Therefore, therapeutic drug monitoring in daily clinical practice is warranted to ensure the therapy's efficacy and prevent bacterial resistance. The purpose of the present study was to develop a method using high‐pressure liquid chromatography with an ultraviolet detector for simultaneous quantification of these four fluoroquinolones in human plasma. A 50 μL aliquot of plasma was precipitated by 200 μL of methanol using gatifloxacin as internal standard. The chromatographic separation was performed on a Kinetex XB‐C18 column using a mobile phase composed of a mixture of orthophosphoric acid 0.4% (v/v), acetonitrile and methanol at a flow rate of 1.2 mL/min. Dual UV wavelength mode was used, with levofloxacin and moxifloxacin monitored at 293 nm, and pefloxacin and ciprofloxacin monitored at 280 nm. The calibration was linear over the ranges of 0.125–25 mg/L for levofloxacin, 0.1–20mg/L for moxifloxacin and 0.05‐10 mg/L for both pefloxacin and ciprofloxacin. Inter‐ and intra‐day trueness and precision were <13% for all the compounds under study. The proposed method was simple, reliable, cost‐effective and suitable for therapeutic drug monitoring or pharmacokinetics studies.

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“…Optimization of a single response with varying all the factors at a single approach, the chemometric analysis makes the best choice of separation (Mannemala et al, 2016), which helps in hasting the method development and extensively explains the chromatographic nature of the eluent. The different approaches to chemometric analysis include the pareto-optimality, path of steepest ascent, Derringer's desirability function and coerce acceleration of the procedure.…”

Section: Introductionmentioning

confidence: 99%

Application of Chemometrics for the simultaneous estimation of stigmasterol and β-sitosterol in Manasamitra Vatakam-an ayurvedic herbomineral formulation using HPLC-PDA method

2018

J App Pharm Sci

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A new research method has been developed to approach multiresponse optimization for simultaneously optimizing a large number of experimental factors. LC Chromatogram was optimized using Phenomenex RP C 18 column (250 x 4.6 mm; 5 µm); mobile phase was surged at isocratic mode with a flow of 1.0 mL/min using methanol and acetonitrile (95:5% v/v) at the detection max of 208 nm with the retention time of 16.3 and 18.1 min for Stigmasterol and β-Sitosterol respectively. Amount of Stigmasterol and β-Sitosterol was quantified and found to be 51.0 and 56.3 µg/ mg respectively. The r 2 value of 0.9971 and 0.9960 was found in the linear range of 80-130 µg/mL for Stigmasterol and β-Sitosterol respectively. LOD and LOQ were 6.951, 21.063 µg/mL and 6.048, 18.328 µg/mL for Stigmasterol and β-Sitosterol respectively. The relative standard deviation for the system and the method precision were found to be 0.94%, 0.40% and 1.51%, 1.1% (≤2%) for stigmasterol and β-Sitosterol. Recovery studies were performed and found in the range of 95-105% indicates the accuracy of the developed method. The developed chromatographic method is the first report for the concurrent estimation of Stigmasterol and β-Sitosterol in Manasamitra Vatakam.

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Multiple response optimization of a liquid chromatographic method for determination of fluoroquinolone and nitroimidazole antimicrobials in serum and urine

Cited by 11 publications

References 27 publications

Development of an icELISA and immunochromatographic strip for detection of norfloxacin and its analogs in milk

Development of an icELISA and immunochromatographic strip for detection of norfloxacin and its analogs in milk

Simultaneous quantification of levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin in microvolumes of human plasma using high‐performance liquid chromatography with ultraviolet detection

Application of Chemometrics for the simultaneous estimation of stigmasterol and β-sitosterol in Manasamitra Vatakam-an ayurvedic herbomineral formulation using HPLC-PDA method

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