Multiple Resistance Phenotypes to <i>Lettuce mosaic virus</i> Among <i>Arabidopsis thaliana</i> Accessions

Revers, Frédéric; Guiraud, Thomas; Houvenaghel, Marie-Christine; Mauduit, Thierry; Gall, Olivier Le; Candresse, Thierry

doi:10.1094/mpmi.2003.16.7.608

Cited by 42 publications

(28 citation statements)

References 44 publications

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“…To date, most of the disease resistance (R) genes isolated [40,41]. In contrast identification of several natural recessive genes that affect virus multiplication in Arabidopsis has been reported from various ecotypes [42][43][44]. Not surprisingly, the genetic basis of resistance is poorly understood and relatively few virus resistance genes have been fully characterized so far.…”

Section: Discussionmentioning

confidence: 99%

Molecular Marker-Assisted Genotyping of Mungbean Yellow Mosaic India Virus Resistant Germplasms of Mungbean and Urdbean

et al. 2010

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Mungbean Yellow Mosaic India Virus (MYMIV) belonging to the genus begomovirus causes the yellow mosaic disease in a number of economically important edible grain legumes including mungbean (Vigna radiata), urdbean (Vigna mungo) and soybean (Glycine max). The disease is severe, critical, open spread and inflicts heavy yield losses annually. The objective of this study is to develop molecular markers linked to MYMIV-resistance to facilitate genotyping of urdbean and mungbean germplasms for MYMIV-reaction. Resistance-linked molecular markers were successfully developed from consensus motifs of other resistance (R) gene or R gene homologue sequences. Applying linked marker-assisted genotyping, plant breeders can carry out repeated genotyping throughout the growing season in absence of any disease incidence. Two MYMIV-resistance marker loci, YR4 and CYR1, were identified and of these two CYR1 is completely linked with MYMIV-resistant germplasms and co-segregating with MYMIV-resistant F₂, F₃ progenies of urdbean. The present study demonstrated that these two markers could be efficiently employed together in a multiplex-PCR-reaction for genotyping both V. mungo and V. radiata germplasms from field grown plants and also directly from the seed stock. This method of genotyping would save time and labour during the introgression of MYMIV-resistance through molecular breeding, as methods of phenotyping against begomoviruses are tedious, labour and time intensive.

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Section: Discussionmentioning

confidence: 99%

Molecular Marker-Assisted Genotyping of Mungbean Yellow Mosaic India Virus Resistant Germplasms of Mungbean and Urdbean

et al. 2010

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“…Inoculation of Arabidopsis plants with both viruses was performed as described by Revers et al (2003) and Mahajan et al (1998). ELISA as described by Revers et al (1997) and RT-PCR using the NIb/P4 primer pair as described by Revers et al (1999b) were used to detect LMV.…”

Section: Virus Inoculation and Detectionmentioning

confidence: 99%

RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein

Cosson

Sofer

et al. 2010

Plant Physiology

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Restriction of long-distance movement of several potyviruses in Arabidopsis (Arabidopsis thaliana) is controlled by at least three dominant restricted TEV movement (RTM) genes, named RTM1, RTM2, and RTM3. RTM1 encodes a protein belonging to the jacalin family, and RTM2 encodes a protein that has similarities to small heat shock proteins. In this article, we describe the positional cloning of RTM3, which encodes a protein belonging to an undescribed protein family of 29 members that has a meprin and TRAF homology (MATH) domain in its amino-terminal region and a coiled-coil domain at its carboxy-terminal end. Involvement in the RTM resistance system is the first biological function experimentally identified for a member of this new gene family in plants. Our analyses showed that the coiled-coil domain is not only highly conserved between RTM3-homologous MATH-containing proteins but also in proteins lacking a MATH domain. The cluster organization of the RTM3 homologs in the Arabidopsis genome suggests the role of duplication events in shaping the evolutionary history of this gene family, including the possibility of deletion or duplication of one or the other domain. Protein-protein interaction experiments revealed RTM3 self-interaction as well as an RTM1-RTM3 interaction. However, no interaction has been detected involving RTM2 or the potyviral coat protein previously shown to be the determinant necessary to overcome the RTM resistance. Taken together, these observations strongly suggest the RTM proteins might form a multiprotein complex in the resistance mechanism to block the long-distance movement of potyviruses.

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“…To determine if this resistance is PPV-specific, two resistant and two susceptible RILs were inoculated with two other potyviruses, LMV-AF199 (for which Cvi is resistant and Ler symptomless susceptible) (Revers et al 2003) and TuMV-UK1 (Cvi and Ler are both susceptible and exhibit symptoms) (Martin Martin et al 1999). The two resistant RILs carried resistance alleles at both loci controlling PPV resistance but lacked the gene controlling resistance to LMV-AF199 infection described by Revers and associates (2003).…”

Section: Resistance Determinants In the CVI × Ler Populationmentioning

confidence: 99%

Identification of Quantitative Trait Loci Controlling Symptom Development During Viral Infection inArabidopsis thaliana

Sicard¹,

Keurentjes²,

Candresse³

et al. 2008

MPMI

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In compatible interactions between plants and viruses that result in systemic infection, symptom development is a major phenotypic trait. However, host determinants governing this trait are mostly unknown, and the mechanisms underlying it are still poorly understood. In a previous study on the Arabidopsis thaliana-Plum pox virus (PPV) pathosystem, we showed a large degree of variation in symptom development among susceptible accessions. Two basic types of interaction exist between a virus and its host plant: i) a compatible interaction associated with plant invasion and viral multiplication, followed in most cases by the induction of symptoms, and ii) an incompatible interaction with no or only limited viral multiplication or movement in the host plant. Plant-virus interactions are relatively well documented, but efforts have so far focused on resistance (or resistancebreaking) mechanisms and on the identification of their genetic determinants. Conversely, the one or more mechanisms controlling symptom development in plants are still largely unknown, so that only a very patchy picture of the interaction between a plant and a virus can be presented.

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Multiple Resistance Phenotypes to Lettuce mosaic virus Among Arabidopsis thaliana Accessions

Cited by 42 publications

References 44 publications

Molecular Marker-Assisted Genotyping of Mungbean Yellow Mosaic India Virus Resistant Germplasms of Mungbean and Urdbean

Molecular Marker-Assisted Genotyping of Mungbean Yellow Mosaic India Virus Resistant Germplasms of Mungbean and Urdbean

RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein

Identification of Quantitative Trait Loci Controlling Symptom Development During Viral Infection inArabidopsis thaliana

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