Multiple reaction monitoring profiling as an analytical strategy to investigate lipids in extracellular vesicles

Abstract

Extracellular vesicles (EVs) convey information used in cell‐to‐cell interactions. Lipid analysis of EVs remains challenging because of small sample amounts available. Lipid discovery using traditional mass spectrometry platforms based on liquid chromatography and high mass resolution typically employs milligram sample amounts. We report a simple workflow for lipid profiling of EVs based on multiple reaction monitoring (MRM) profiling that uses microgram amounts of sample. After liquid–liquid extraction, individual EV samples were injected directly into the electrospray ionization (ESI) ion source at low flow rates (10 μl/min) and screened for 197 MRM transitions chosen to be a characteristic of several classes of lipids. This choice was based on a discovery experiment, which applied 1,419 MRMs associated with multiple lipid classes to a representative pooled sample. EVs isolated from 12 samples of human lymphocytes and 16 replicates from six different rat cells lines contained an estimated amount of total lipids of 326 to 805 μg. Samples showed profiles that included phosphatidylcholine (PC), sphingomyelin (SM), cholesteryl ester (CE), and ceramide (Cer) lipids, as well as acylcarnitines. The lipid profiles of human lymphocyte EVs were distinguishable using principal component and cluster analysis in terms of prior antibody and drug exposure. Lipid profiles of rat cell lines EV's were distinguishable by their tissue of origin.

Modulation of Pulmonary Toxicity in Metabolic Syndrome Due to Variations in Iron Oxide Nanoparticle-Biocorona Composition

“…Following BC formation and isolation of NPs by centrifugation, NP-associated lipids were extracted from the NP surface via a modified Bligh-Dyer lipid extraction method [34]. The MRMprofiling methods and instrumentation used have been recently described in previous reports [33,[35][36][37][38]. Specifically, isolated NP-BCs (described previously) were resuspended in 200 µL water following by adding 125 µL of chloroform and 250 µL of methanol.…”

Targeted Mass Spectrometry Reveals Interferon-Dependent Eicosanoid and Fatty Acid Alterations in Chronic Myeloid Leukaemia

“…In general, lipids are challenging molecules to characterise due to the limited analytical capacity to identify, quantify and annotate them in biological samples, which has been an impediment to the advancement of discovering molecular mechanisms of disease, biomarker discovery and drug development. Mass spectrometry (MS) approaches, predominantly in combination with liquid chromatography (LC), are widely used for the analysis of biomolecules [20][21][22][23], including eicosanoids [24,25]. More recently, LC has been coupled with dynamic multiple reaction monitoring (dMRM) workflows for the targeted detection of specific subsets of eicosanoids [26,27], and high-resolution (MRMHR) methods to generate a library of high-resolution fragmentation spectra [28] have been developed.…”