Multiple precipitation of sera containing monoclonal IgG proteins in radial immunodiffusion

Mulder, Jan; Verhaar, M.A.T.

doi:10.1016/0009-8981(73)90032-6

Cited by 8 publications

(3 citation statements)

References 14 publications

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“…According to Lietze (6) and Mulder (7)(8), multiple precipitation, noticed in pathological samples in single radial immunodiffusion is either due to the blocking of antigenic determinants by other bound proteins, or due to the lack of antigenic determinants in the individual immunoglobulins.…”

Section: Resultsmentioning

confidence: 98%

Bound and Free Light Chains in Subacute Sclerosing Panencephalitis and Multiple Sclerosis Serum and Cerebrospinal Fluid

Bollengier¹,

Löwenthal²,

Henrotin³

1975

Clinical Chemistry and Laboratory Medicine

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The kappa-lambda light chain ratios, the presence of free light chains and the double ring formation, with antikappa and antilambda serum, in single radial immunodiffusion were investigated in serum and cerebrospinal fluid of patients with subacute sclerosing panencephalitis.Cerebrospinal fluid samples of several multiple sclerosis cases were considered simultaneously. The results obtained suggest special immunoglobulin synthesis in both diseases. Gebundene und freie leichte Ketten in Serum und Liquor cerebrospinalis bei Subakuter Sklerosierender Panenceplmlitis und Multipler SkleroseZusammenfassung: In Serum und Liquor cerebrospinalis von Patienten mit subakuter Sklerosierender Panencephalitis wurden die Verhältnisse von leichten Kappa-zu leichten Lambda-Ketten und die Doppelring-Bildung mit anti-Kappaund anti-Lambda-Serum in der einfachen radialen Immundiffusion untersucht.Liquorproben zahlreicher Fälle von Multipler Sklerose wurden ebenfalls untersucht. Die Ergebnisse sprechen für eine spezielle Imunnglobulinsynthese bei beiden Erkrankungen.

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Section: Resultsmentioning

confidence: 98%

Bound and Free Light Chains in Subacute Sclerosing Panencephalitis and Multiple Sclerosis Serum and Cerebrospinal Fluid

Bollengier¹,

Löwenthal²,

Henrotin³

1975

Clinical Chemistry and Laboratory Medicine

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“…In the case ofIgG all our double zones have occurred in patients later found to have myeloma. In one case the double zone regressed to a single zone with therapy as has been described by Mulder and Verhaar (1973).…”

mentioning

confidence: 87%

“…An attempt to quantitate the two zones by direct measurement has proved unsuccessful and is possibly further evidence of abnormal binding sites in the abnormal protein. Studies of this phenomenon by Mulder and Verhaar (1973) relate the findings with IgG to a deficiency of specific antibody determinants to the Fd fragment of the monoclonal proteins. In the case ofIgG all our double zones have occurred in patients later found to have myeloma.…”

mentioning

confidence: 99%

Evaluation of Specific Protein Analysis in a Non-Teaching Hospital Laboratory

Salkie

McNeil

1974

Ann Clin Biochem

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A retrospective study has been made to assess the value of specific protein analysis in a routine laboratory. The use of cellulose acetate electrophoresis, immunoelectrophoresis, and single radial immunodiffusion has been discussed with reference to the source of the request for the investigation and the yield of abnormal results. A scheme for the investigation of abnormal proteins is presented with a discussion of the problem of diagnosing myeloma as distinct from identifying an abnormal protein. The occurrence of double-zones on Tri-Partigen plates (Hoechst Pharm.) may be an early indication of myeloma, but does occur in other conditions. Levels of immunoglobulins in the geriatric age group have been shown to be similar to that of non-geriatric adults. Specific protein analysis has been shown to be of limited value in rheumatoid arthritis.

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Heterogeneity of monoclonal immunoglobulin-G proteins studied by isoelectric focusing

Brendel

Mulder

Verhaar

1974

Clinica Chimica Acta

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Multiple precipitation of sera containing monoclonal IgG proteins in radial immunodiffusion

Cited by 8 publications

References 14 publications

Bound and Free Light Chains in Subacute Sclerosing Panencephalitis and Multiple Sclerosis Serum and Cerebrospinal Fluid

Bound and Free Light Chains in Subacute Sclerosing Panencephalitis and Multiple Sclerosis Serum and Cerebrospinal Fluid

Evaluation of Specific Protein Analysis in a Non-Teaching Hospital Laboratory

Heterogeneity of monoclonal immunoglobulin-G proteins studied by isoelectric focusing

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