Multiple plant hormones and cell wall metabolism regulate apple fruit maturation patterns and texture attributes

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (<i>Malus</i> × <i>domestica</i>) Fruit Maturation Patterns

“…Based on the previous apple transcriptome analysis on apple fruit ripening [14], unigene and EST sequences with annotated function of auxin conjugation and auxin transporter were identified from the apple genome sequences by performing a BLASTN search against Apple Genome V 1.0 predicted CDS sequences in the Genome Database for Rosaceae website (www.rosaceae.org) [48]. Name of the genes were followed [13] [37].…”

“…MdPIN1 (MDP0000138035) was identified from apple genome sequences based on the sequence similarity with two contigs and one singlet identified from the previous microarray anlysis [14]. Similarly, the full-length sequences of a MdAECFP1 (auxin efflux carries family protein, MDP0000344085) and two genes encoding enzymes functioning in auxin and amino acid conjugation [MdGH3.102 (MDP0000872868) and MdGH3.18 (MDP0000811081)] were identified based on the identified EST sequences [14]. For tracking the activation of the ethylene biosynthesis pathway and potential correlation between auxin and ethylene function during apple fruit maturation, two ethylene biosynthesis genes, i.e.…”

“…However, ethylene itself is most likely just one node in the hormone regulation network. Recent transcriptomic and genomic studies indicated the roles of auxin and its potential interactions with ethylene during apple fruit maturation and ripening [13] [14], but the details of the interactions are unknown.…”

“…In the current study, based on our previous transcriptome analysis of apple fruit ripening [14], the gene families of auxin efflux carrier and IAA-amido synthetase were identified from the apple genome sequences [48]. The expression profiles of apple auxin efflux carriers (MdPIN1 and MdAECFP1) and IAA-amido synthetases (MdGH3.102 and MdGH3.18), as well as ethylene biosynthesis genes (MdACS3 and MdACS1), were characterized using the qRT-PCR (quantitative reverse transcription polymerase chain reaction).…”

Identification of Differentially Expressed Genes between “Honeycrisp” and “Golden Delicious” Apple Fruit Tissues Reveal Candidates for Crop Improvement

“…With respect to probing the changes in apple gene expression, several studies have used microarray analysis of gene expression [1,51,52]. With the issues associated with microarray accuracy and reproducibility [53], expanding genomic resources available for Rosaceae, and increased access to next-generation sequence technologies, RNA-seq is emerging as the gold standard to identify transcriptional differences between apple cultivars.…”