Differential Suppression of Ethylene Biosynthesis and Receptor Genes in ‘Golden Delicious’ Apple by Preharvest and Postharvest 1-MCP Treatments

Varanasi, Vijaya; Shin, Sungbong; Johnson, Franklin; Mattheis, James P.; Zhu, Yanmin

doi:10.1007/s00344-013-9326-8

Cited by 36 publications

(26 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The sequences of the amplified regions seem to be identical between these two cultivars using qRT-PCR method. The internal reference of an apple MdActin (MDP0000752428) has been used in several previous studies and its consistency among various tissue types and treatments has been tested in several studies [54]- [56]. Additionally, use of "adaptive calibrator" for calculating the relative expression values enhances the comparability of the relative gene expression levels between two cultivars.…”

Section: Identified Auxin Efflux Carrier and Iaa-amido Synthetase Genmentioning

confidence: 99%

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (Malus × domestica) Fruit Maturation Patterns

Shin¹,

Lee²,

Rudell³

et al. 2015

AJPS

Self Cite

View full text Add to dashboard Cite

Auxin has been suggested to play an essential role in regulating apple fruit maturation and ripening, though the molecular function of auxin and its interaction with ethylene during apple fruit development are largely unknown. To understand the function of auxin during apple fruit maturation and ripening, auxin efflux carrier and IAA-amido synthetase encoding genes were identified from the apple genome based on the results of previous microarray analysis. The expression patterns of these genes were analyzed using qRT-PCR during 10 -12 weeks of fruit maturation for two apple cultivars: "Golden Delicious" (GD) and "Cripps Pink" (CP), which have the distinct patterns of maturation progression. Our results showed that the expressions of auxin efflux carrier and IAA-amido synthetase genes have a correlation with the timing of ethylene biosynthesis pathway activation in both cultivars. The earlier and stronger expression of MdGH3.102 and MdAECFP1 in the fruit of GD, a mid-season cultivar, correlates with the earlier activation of a pre-climacteric ethylene biosynthesis gene of MdACS3, compared with that in CP, a late-ripening apple cultivar. Results of exogenous IAA treatment indicated that the expression patterns of the genes were regulated in a fruit maturity dependent manner. Our results suggested that the dynamics of the auxin level in apple fruit cortex could be one of the key factors influencing the timing of ethylene biosynthesis pathway activation and consequently contributed to the control of the apple maturation progression.

show abstract

Section: Identified Auxin Efflux Carrier and Iaa-amido Synthetase Genmentioning

confidence: 99%

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (Malus × domestica) Fruit Maturation Patterns

Shin¹,

Lee²,

Rudell³

et al. 2015

AJPS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Estes resultados são indicativos de que a aplicação combinada 1-MCPa+1-MCPg não é superior à aplicação isolada do 1-MCPg. No entanto, em maçãs 'Golden Delicious', a combinação 1-MCPa+1-MCPg resultou em maior conservação da firmeza de polpa e menor produção de etileno do que o tratamento 1-MCPg (Varanasi et al, 2013). Interessante notar que, assim como observado em maçãs (McArtney et al, 2008), a perda progressiva dos efeitos do 1-MCPa, com o atraso da colheita após a aplicação, foi recuperada pela aplicação do 1-MCPg logo após a colheita.…”

Section: Resultsunclassified

“…Ademais, a penetração de 1-MCPa no fruto é reduzida pela menor difusão do 1-MCP em água e pela obstrução das lenticelas pela água . Finalmente, diferenças quanto à eficiência do 1-MCPa e 1-MCPg podem estar relacionadas ao fato de que alguns dos receptores de etileno inibidos pelo 1-MCPg podem não ser inibidos pelo 1-MCPa, conforme mostrado em maçãs (Varanasi et al, 2013).…”

Section: Resultsunclassified

“…No entanto, esses efeitos são dependentes da cultivar, local, ano agrícola (ou safra) e estádio de maturação dos frutos. Além disso, foi relatada resposta diferencial do 1-MCPa, quando aplicado em pré e em pós-colheita (Varanasi et al, 2013). Exemplo disto ocorre em maçã 'Honeycrisp', em que o tratamento pós-colheita com 1-MCPg não teve efeito sobre a ocorrência do distúrbio escaldadura profunda, enquanto a pulverização pré-colheita de 1-MCPa reduziu esse distúrbio (DeEll et al, 2010).…”

Section: Introductionunclassified

“…Exemplo disto ocorre em maçã 'Honeycrisp', em que o tratamento pós-colheita com 1-MCPg não teve efeito sobre a ocorrência do distúrbio escaldadura profunda, enquanto a pulverização pré-colheita de 1-MCPa reduziu esse distúrbio (DeEll et al, 2010). Em algumas culturas, o que inclui a maçã (Elfving et al, 2007;McArtney et al, 2008;Varanasi et al, 2013;Scolaro et al, 2015) e o kiwi (Manríquez & Defilippi, 2011), o tratamento pré-colheita com 1-MCPa também pode promover aumento da conservação da qualidade pós-colheita dos frutos.…”

Section: Introductionunclassified

See 2 more Smart Citations

Conservação de caqui 'Fuyu' com o tratamento em pré-colheita e pós-colheita com 1-metilciclopropeno

Vieira

Argenta

Amarante

et al. 2016

Pesq. agropec. bras.

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi comparar os efeitos da pulverização pré-colheita de 1-metilciclopropeno aquoso (1-MCPa) e tratamento pós-colheita de 1-metilciclopropeno gasoso (1-MCPg) sobre a maturação e a qualidade de caqui (Diospyros kaki) 'Fuyu'. Pulverizaram-se 150 mg L -1 de 1-MCPa nas plantas, na primeira semana de colheita comercial. Os frutos foram colhidos aos 1, 7, 14 e 21 dias, após a pulverização do 1-MCPa (DAAM), e tratados ou não com 0,6 µL L -1 de 1-MCPg, um dia após a colheita. Os tratamentos constituíram-se de controle, 1-MCPa, 1-MCPg e 1-MCPa+1-MCPg. Os frutos foram armazenados por 70 dias a 0 o C, em atmosfera modificada (AM), seguido por sete dias a 23ºC. O tratamento com 1-MCPa não afetou a maturação dos frutos na planta, mas reduziu o amolecimento prematuro de polpa e o distúrbio mancha translúcida em pós-colheita. A incidência de frutos moles após a armazenagem (média de três anos) foi superior a 84% no controle e inferior a 18% com 1-MCPg, independentemente da época de colheita, e de 15, 34, 40 e 75%, quando os frutos foram tratados com 1-MCPa e colhidos 1, 7, 14 e 21 DAAM, respectivamente. A eficácia do 1-MCPa em frutos colhidos 1 DAAM é semelhante à do 1-MCPg e é reduzida com o atraso da colheita dos frutos a partir da data de sua aplicação.Termos para indexação: Diospyros kaki, atmosfera modificada, distúrbios fisiológicos, etileno, fruto, dano por frio. Fruit quality preservation of 'Fuyu' persimmon by preharvest and postharvest treatments with 1-methylcyclopropeneAbstract -The objective of this work was to compare the effects of preharvest spraying of aqueous 1-methylcyclopropene (1-MCPa) and of postharvest treatment of gaseous 1-methylcyclopropene (1-MCPg) on fruit maturity and quality of 'Fuyu' persimmon (Diospyros kaki). Spraying of 150 mg L -1 1-MCPa was done on trees in the first week of commercial harvesting. Fruit were harvested at 1, 7, 14, and 21 days after 1-MCPa spraying (DAMS), and treated or not with 0.6 µL L -1 1-MCPg one day after harvest. The treatments consisted of a control, 1-MCPa, 1-MCPg, and 1-MCPa+1-MCPg. Fruit were stored at 0°C for 70 days in modified atmosphere, followed by seven days at 23°C. The treatment with 1-MCPa did not affect fruit maturity on trees, but reduced the premature flesh softening and the occurrence of translucent stain disorder at postharvest. The incidences of soft fruit after storage (average of three years) were higher than 84% for the control, and lower than 18% for fruit treated with 1-MCPg, regardless of fruit harvesting date, and they were 15, 34, 40, and 75% for fruit treated with 1-MCPa and harvested at 1, 7, 14, and 21 DAMS, respectively. The efficacy of 1-MCPa is equivalent to that of 1-MCPg for fruit harvested at 1 DAMS and its is reduced by delaying fruit harvest from the date of its application.

show abstract

Influence of Postharvest Technologies and Handling Practices on Phytochemicals in Fruits and Vegetables

Yahia

Serrano

Valero

et al. 2017

Fruit and Vegetable Phytochemicals

View full text Add to dashboard Cite

Differential Suppression of Ethylene Biosynthesis and Receptor Genes in ‘Golden Delicious’ Apple by Preharvest and Postharvest 1-MCP Treatments

Cited by 36 publications

References 52 publications

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (<i>Malus</i> × <i>domestica</i>) Fruit Maturation Patterns

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (<i>Malus</i> × <i>domestica</i>) Fruit Maturation Patterns

Conservação de caqui 'Fuyu' com o tratamento em pré-colheita e pós-colheita com 1-metilciclopropeno

Influence of Postharvest Technologies and Handling Practices on Phytochemicals in Fruits and Vegetables

Contact Info

Product

Resources

About

Differential Suppression of Ethylene Biosynthesis and Receptor Genes in ‘Golden Delicious’ Apple by Preharvest and Postharvest 1-MCP Treatments

Cited by 36 publications

References 52 publications

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (&lt;i&gt;Malus&lt;/i&gt; &#215; &lt;i&gt;domestica&lt;/i&gt;) Fruit Maturation Patterns

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (&lt;i&gt;Malus&lt;/i&gt; &#215; &lt;i&gt;domestica&lt;/i&gt;) Fruit Maturation Patterns

Conservação de caqui 'Fuyu' com o tratamento em pré-colheita e pós-colheita com 1-metilciclopropeno

Influence of Postharvest Technologies and Handling Practices on Phytochemicals in Fruits and Vegetables

Contact Info

Product

Resources

About

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (<i>Malus</i> × <i>domestica</i>) Fruit Maturation Patterns

Transcript Profiles of Auxin Efflux Carrier and IAA-Amido Synthetase Genes Suggest the Role of Auxin on Apple (<i>Malus</i> × <i>domestica</i>) Fruit Maturation Patterns