Multiple Metastases of a Transmissible Venereal Tumor in a Dog

Abstract

Background: Transmissible venereal tumor (TVT) is a common contagious neoplasm in dogs that spreads through coitus. Extra-genital presentations of this tumor are frequent and usually develop through implantation of neoplastic cells on exposed mucosae. TVT metastasis is rare, and when it happens it's usually affecting regional lymph nodes and adjacent cutaneous tissue. Case: A female mixed breed dog, with estimated age of 7 to 11 months old, was rescued from the streets and taken to a veterinary clinic in the city of Porto Alegre, RS. The animal had multiple nodules on its body, vulva, ocular mucosa, and gingiva, along with signs of malnutrition and apathy. Cytological examination of the nodules and vulva was done and yielded a cytologic picture compatible with TVT. Weakly treatment with 0,3mg/m² vincristine sulphate was used until clinical cure was noted. Approximately two weeks after clinical cure, the dog showed a blue colored eye and was referred for ophthalmological, where it was diagnosed with vision loss due to glaucoma secondary to a neoplasm. The eye was then removed and sent for histopathological evaluation. Histopathology of the eye was compatible with TVT diagnosis. One month after enucleation the animal display dispenia, pain, aggressiveness and epistaxis. The animal was euthanized and submitted for post-mortem evaluation. At necropsy there was a well-defined grayish-white, nodule near the thalamus. Similar nodules were also found on the lung, and anterior chamber of the eye. Histologically, all the nodules were compatible with TVT. Immunohistochemical examination was done, with the neoplastic cells being positive for vimentin and negative for cytokeratin, CD79a, CD3 and CD117. Based on the post-mortem examination and clinical history, diagnosis of TVT was given. Discussion: The clinical manifestation of the tumor in the genitalia presented by the animal is characteristic of TVT, but the extragenital presentation is less common. Although extragenital manifestations are well reported, most are due to auto-implantation (contact with the dog's own genitalia) or hetero-implantation (contact with the genitalia of another dog). Metastases originating from the genitalia are markedly less common (5% of cases), and when they occur, they usually affect regional lymph nodes due to lymphatic communication. However, they can also occur in other organs, such as the liver, kidneys, spleen, tonsils, skin and subcutaneous tissue, bone, CNS, mesentery, and eyes. In this case, it is difficult to determine if the ocular tumor reported was due to metastasis or implantation by direct eye contact with TVT cells, since the animal lived on the street, and it was not possible to establish a more accurate history. The dog in this study was treated with vincristine sulfate; however, the treatment was stopped after one month, when no signs of the tumor were observed. Treatment with vincristine is the method of choice for TVT, since it is highly effective. However, rare cases of recurrence after treatment may occur. In addition, t...

Treatment of transmissible venereal tumor without metastasis in mixed local Balinese dog by surgery and vincristine sulfate: A case report

“…Surgery is effective if the TVT case is at stage I, the tumor nodule is small, where the tumor is easily accessible, and is non-invasive (Martins et al, 2005) [11] . TVT metastases are very rare, and when they do occur they usually affect the regional lymph nodes and surrounding cutaneous tissue (Faccini et al, 2019) [6] . Vincristine sulfate has been used widely as a single chemotherapy agent or combination therapy for the management of TVT cases.…”

Tumor venéreo transmissível em cavidade abdominal de cadela: Relato incomum

“…Apesar da quimioterapia com Sulfato de Vincristina ser um método eficaz para tratamento de TVT, pode ocorrer resistência ou recidiva após o término do protocolo (Faccini et al, 2019;Setthawongsin et al, 2016). Durante dois meses foi realizado o acompanhamento ultrassonográfico e não foram visibilizadas alterações compatíveis com metástases.…”

Canine transmissible veneral tumor:etiology, diagnosis and treatment

“…Lesions are fragile and mostly hemorrhagic as a result of low cohesion between neoplastic cells. Therefore, the first clinical sign is tend to be bleeding (Ferreira et al, 2000;Faccini et al, 2019). Tumor size ranges from millimeters (mm) to several centimeters (cm) with a dark red to grayish pink color (Das and Das, 2000;Purohit, 2009;Lopes et al, 2015).…”

“…As compared with other round cell tumors, TVT has a microscopic appearance to diagnose. Immunohistochemistry can be used to differentiate this neoplasm from other round cell tumors (Ferreira et al, 2000;Faccini et al, 2019). Prooestrus bleeding, cystitis, urethritis and prostatitis should be considered in the differential diagnosis (Das and Das, 2000).…”

“…Therefore, in primal TVT cases, it is possible to see lesions in the skin, face, nose or oral cavity, eyes and subcutaneous tissues along with the external genital area (Rogers, 1997;Das and Das, 2000;Brandao et al, 2002;Uçar, 2016;Kumar et al, 2018). Although tumors are located in the genital tract, extragenital cases have been observed including the conjunctival mucosa (Ferreira et al, 2000;Rodrigues et al, 2001) and central nervous system (Ferreira et al, 2000;Faccini et al, 2019). The presence of tumors involving the ocular area is less common than others, and TVT metastases occur by contact in these sides (Ginel et al, 1995;Souza et al, 2020).…”