Multiple Mechanisms of Tigecycline Resistance in
            <i>Enterobacteriaceae</i>
            from a Pig Farm, China

Wang, Jing; Wu, Han; Mei, Cai-Yue; Wang, Yan; Wang, Zhenyu; Lu, Meng-Jun; Pan, Zhiming; Jiao, Xinan

doi:10.1128/spectrum.00416-21

Cited by 15 publications

(7 citation statements)

References 16 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pigs have been found to be important vehicles of tet (X4)-producing E. coli isolates, which is consistent with the results from previous studies [ 4 , 5 , 8 , 16 ]. Furthermore, the pig tet (X4)-producing E. coli isolates from Shaanxi differed by only 119 SNPs from the human isolates in a previous study from China [ 8 ].…”

Section: Discussionsupporting

confidence: 91%

International Spread of Tet(X4)-Producing Escherichia coli Isolates

Zhang

Zhan

Shi

2022

Foods

View full text Add to dashboard Cite

Tigecycline resistance in bacteria has become a significant threat to food safety and public health, where the development of which is attributed to plasmid-mediated tet(X4) genes. In this study, the genomes of 613 tet(X4)-producing Escherichia coli (E. coli) isolates, available from public databases, are evaluated to determine their international prevalence and molecular characterization. These E. coli isolates have been disseminated in 12 countries across Asia and Europe. It was found that pigs and their products (n = 162) were the most common vehicle, followed by humans (n = 122), chickens (n = 60), and the environment (n = 49). Carbapenems-resistant genes blaNDM-5 (1.3%) and blaNDM-1 (0.2%) were identified, as well as colistin-resistant genes mcr-1.1 (12.6%) and mcr-3.1 (0.5%). It was noted that the tigecycline-resistant gene cluster tmexC-tmexD-toprJ1 was identified in seven (1.1%) isolates. Phylogenomic results indicated that tet(X4)-producing E. coli isolates fell into seven lineages (lineages I, II, III, IV, V, VI, and VII), and international spread mainly occurred in Asian countries, especially China, Pakistan, Singapore, and Malaysia. Four forms of tet(X4) transposon units were found, including the I-type (IS26-tet(X4)-ISCR2), II-type (ΔIS1R-tet(X4)-ISCR2), III-type (ISCR2-tet(X4)-ISCR2), and IV-type (ISCR2-tet(X4)-ΔISCR2). These findings underline further challenges for the spread of E. coli bearing tet(X4) gene.

show abstract

Section: Discussionsupporting

confidence: 91%

International Spread of Tet(X4)-Producing Escherichia coli Isolates

Zhang

Zhan

Shi

2022

Foods

View full text Add to dashboard Cite

show abstract

“…Tet (X4)-positive strains have spread globally and have been detected in animals, humans and the environment, which largely limited the use of tigecycline ( Xu et al, 2022 ). The tet (X) gene and its variants were present in 23 countries on six continents ( Pan et al, 2020 ; Wang J. et al, 2021 ), which are also widely present in various bacterial species, including R. anatipestifer , E. coli , Acinetobacter , K. pneumoniae , Salmonella , Proteus , La Providencia bacteria, Bacteroides bacteria , Pseudomonas bacteria , and Aeromonas caviae ( Chen et al, 2019a , 2020 ). Moreover, most of the tet (X4) genes are located on different types of plasmids such as IncQ1, IncX1, IncFIB, IncHI1, F-:A18:B-, ColE2-like, IncN, p0111 and hybrid plasmids ( Fang et al, 2020 ), among which the IncX1 type is the most common ( Cai et al, 2021 ; Cui et al, 2022 ).…”

Section: Prevalence Of Tet (X4)mentioning

confidence: 99%

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Zhang

Wen²,

Wang³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

With the large-scale use of antibiotics, antibiotic resistant bacteria (ARB) continue to rise, and antibiotic resistance genes (ARGs) are regarded as emerging environmental pollutants. The new tetracycline-class antibiotic, tigecycline is the last resort for treating multidrug-resistant (MDR) bacteria. Plasmid-mediated horizontal transfer enables the sharing of genetic information among different bacteria. The tigecycline resistance gene tet(X) threatens the efficacy of tigecycline, and the adjacent ISCR2 or IS26 are often detected upstream and downstream of the tet(X) gene, which may play a crucial driving role in the transmission of the tet(X) gene. Since the first discovery of the plasmid-mediated high-level tigecycline resistance gene tet(X4) in China in 2019, the tet(X) genes, especially tet(X4), have been reported within various reservoirs worldwide, such as ducks, geese, migratory birds, chickens, pigs, cattle, aquatic animals, agricultural field, meat, and humans. Further, our current researches also mentioned viruses as novel environmental reservoirs of antibiotic resistance, which will probably become a focus of studying the transmission of ARGs. Overall, this article mainly aims to discuss the current status of plasmid-mediated transmission of different tet(X) genes, in particular tet(X4), as environmental pollutants, which will risk to public health for the “One Health” concept.

show abstract

“…Specifically, the activity of Tet(X) resistance enzyme was lower than 10% after treatment with 1,000 μg/mL Bi(NO 3 ) 3 . To further verify whether the synergy of Bi(NO 3 ) 3 is related only to tet (X)-mediated tigecycline resistance, we tested the synergistic activity between bismuth nitrate and tigecycline against tmexCD1-toprJ1 - or tet (A) variant-mediated tigecycline-resistant bacteria ( 24 , 25 ). However, no direct synergistic activity (FICI values of >0.5) was found in these strains (Fig.…”

Section: Resultsmentioning

confidence: 99%

Bismuth Drugs Reverse Tet(X)-Conferred Tigecycline Resistance in Gram-Negative Bacteria

et al. 2022

View full text Add to dashboard Cite

Recently, high-level tigecycline resistance mediated by tet (X4) and its variants represents a serious challenge for global public health. Antibiotic adjuvant strategy that enhances the activity of the existing antibiotics by using nonantibiotic drugs offers a distinct approach to combat the antibiotic resistance crisis. In this study, we found that bismuth drugs involve bismuth nitrate, a compound previously approved for treatment of stomach-associated diseases, remarkably potentiates tigecycline activity against tet (X)-positive bacteria.

show abstract

Multiple Mechanisms of Tigecycline Resistance in Enterobacteriaceae from a Pig Farm, China

Cited by 15 publications

References 16 publications

International Spread of Tet(X4)-Producing Escherichia coli Isolates

International Spread of Tet(X4)-Producing Escherichia coli Isolates

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Bismuth Drugs Reverse Tet(X)-Conferred Tigecycline Resistance in Gram-Negative Bacteria

Contact Info

Product

Resources

About