International Spread of Tet(X4)-Producing Escherichia coli Isolates

Zhang, Zengfeng; Zhan, Zeqiang; Shi, Chunlei

doi:10.3390/foods11142010

Cited by 9 publications

(8 citation statements)

References 20 publications

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“…It has been found that the IS CR2 element is frequent adjacent to tet (X4) or other tet (X) variants, which suggests IS CR2 is more likely to participate in spread of tet (X) variants ( Wang L. et al, 2019 ; Liu et al, 2020 ; Fu et al, 2021 ). In a conserved genetic environment and uncertain transferability among different bacteria, the co-action of IS CR2 and IS 26 may be the main driving forces for the widespread of tet (X4; Dai et al, 2022 ; Zhang et al, 2022 ).…”

Section: Origin and Spread Of Tet (X4)mentioning

confidence: 99%

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Zhang

Wen²,

Wang³

et al. 2022

Front. Microbiol.

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With the large-scale use of antibiotics, antibiotic resistant bacteria (ARB) continue to rise, and antibiotic resistance genes (ARGs) are regarded as emerging environmental pollutants. The new tetracycline-class antibiotic, tigecycline is the last resort for treating multidrug-resistant (MDR) bacteria. Plasmid-mediated horizontal transfer enables the sharing of genetic information among different bacteria. The tigecycline resistance gene tet(X) threatens the efficacy of tigecycline, and the adjacent ISCR2 or IS26 are often detected upstream and downstream of the tet(X) gene, which may play a crucial driving role in the transmission of the tet(X) gene. Since the first discovery of the plasmid-mediated high-level tigecycline resistance gene tet(X4) in China in 2019, the tet(X) genes, especially tet(X4), have been reported within various reservoirs worldwide, such as ducks, geese, migratory birds, chickens, pigs, cattle, aquatic animals, agricultural field, meat, and humans. Further, our current researches also mentioned viruses as novel environmental reservoirs of antibiotic resistance, which will probably become a focus of studying the transmission of ARGs. Overall, this article mainly aims to discuss the current status of plasmid-mediated transmission of different tet(X) genes, in particular tet(X4), as environmental pollutants, which will risk to public health for the “One Health” concept.

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Section: Origin and Spread Of Tet (X4)mentioning

confidence: 99%

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Zhang

Wen²,

Wang³

et al. 2022

Front. Microbiol.

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“…Colistin, carbapenems and tigecycline are considered last-resort antibiotics for defense against MDR bacterial infections ( Tang et al, 2021 ). However, these antibiotics become less effective because their respective predominant resistance genes, mcr , bla NDM and tet(X) , emerge and spread in animals, food and humans ( Singh et al, 2018 ; Zhong et al, 2019 ; Anyanwu et al, 2022 ; Lu et al, 2022 ; Zhang et al, 2022 ). Among these last-resort antibiotics, colistin was heavily used in food-producing animal industries in China for a long period of time until it was banned as a growth promoter in 2017 ( Walsh and Wu, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…Among the currently available antibiotics, carbapenems, colistin as well as tigecycline are last-resort antibiotics for managing MDR bacterial infections, especially those caused by Enterobacteriaceae ( Tang et al, 2021 ). Unfortunately, the worrying emergence of the predominantly plasmid-borne colistin resistance gene mcr-1 , carbapenem resistance gene bla NDM-1 as well as tigecycline resistance gene tet(X4) in Enterobacteriaceae pathogens from different sources, such as animals, food and humans, has increasingly been reported in different continents ( Singh et al, 2018 ; Zhong et al, 2019 ; Anyanwu et al, 2022 ; Lu et al, 2022 ; Zhang et al, 2022 ). Additionally, tet(X) genes have regularly been reported to coexist with bla NDM-1 and/or mcr-1 genes ( Chen et al, 2019 ; Sun C. et al, 2019 ), and such plasmid-borne antibiotic resistance genes (ARGs) are capable of transferring between epidemic strains of Enterobacteriaceae ( Leshaba et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of multiple resistance genes to last-resort antibiotics in Enterobacteriaceae pathogens by recombinase polymerase amplification combined with lateral flow dipstick

Wang

Pan³

et al. 2023

Front. Microbiol.

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The worrying emergence of multiple resistance genes to last-resort antibiotics in food animals and human populations throughout the food chain and relevant environments has been increasingly reported worldwide. Enterobacteriaceae pathogens are considered the most common reservoirs of such antibiotic resistance genes (ARGs). Thus, a rapid, efficient and accurate detection method to simultaneously screen and monitor such ARGs in Enterobacteriaceae pathogens has become an urgent need. Our study developed a recombinase polymerase amplification (RPA) assay combined with a lateral flow dipstick (LFD) for simultaneously detecting predominant resistance genes to last-resort antibiotics of Enterobacteriaceae pathogens, including mcr-1, blaNDM-1 and tet(X4). It is allowed to complete the entire process, including crude DNA extraction, amplification as well as reading, within 40 min at 37°C, and the detection limit is 101 copies/μl for mcr-1, blaNDM-1 and tet(X4). Sensitivity analysis showed obvious association of color signals with the template concentrations of mcr-1, blaNDM-1 and tet(X4) genes in Enterobacteriaceae pathogens using a test strip reader (R2 = 0.9881, R2 = 0.9745, and R2 = 0.9807, respectively), allowing for quantitative detection using multiplex RPA-LFD assays. Therefore, the RPA-LFD assay can suitably help to detect multiple resistance genes to last-resort antibiotics in foodborne pathogens and has potential applications in the field.

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“…Among the identified tet (X) variants that can degrade tigecycline, tet (X4) is recognized as a highly prevalent plasmid-borne variant that exhibits high enzymatic activity and can mediate high resistance to tigecycline (16–32 mg/mL) (He et al 2019 ). To date, tet (X4)-positive strains have been identified in animals (Wang et al 2022a , b ), foods (Sun et al 2021 ), the environment (Cui et al 2020 ), and humans (Cui et al 2022 ; Zhang et al 2022a , b ). Gradually, these strains are increasingly being reported worldwide.…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of rapid and accurate one-tube RPA-CRISPR-Cas12b-based detection of mcr-1 and tet(X4)

Wang,

Chen,

Pan

et al. 2024

Appl Microbiol Biotechnol

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The emergence and quick spread of the plasmid-mediated tigecycline resistance gene tet(X4) and colistin resistance gene mcr-1 have posed a great threat to public health and raised global concerns. It is imperative to develop rapid and accurate detection systems for the onsite surveillance of mcr-1 and tet(X4). In this study, we developed one-tube recombinase polymerase amplification (RPA) and CRISPR-Cas12b integrated mcr-1 and tet(X4) detection systems. We identified mcr-1- and tet(X4)-conserved and -specific protospacers through a comprehensive BLAST search based on the NCBI nt database and used them for assembling the detection systems. Our developed one-tube RPA-CRISPR-Cas12b-based detection systems enabled the specific detection of mcr-1 and tet(X4) with a sensitivity of 6.25 and 9 copies within a detection time of ~ 55 and ~ 40 min, respectively. The detection results using pork and associated environmental samples collected from retail markets demonstrated that our developed mcr-1 and tet(X4) detection systems could successfully monitor mcr-1 and tet(X4), respectively. Notably, mcr-1- and tet(X4)-positive strains were isolated from the positive samples, as revealed using the developed detection systems. Whole-genome sequencing of representative strains identified an mcr-1-carrying IncI2 plasmid and a tet(X4)-carrying IncFII plasmid, which are known as important vectors for mcr-1 and tet(X4) transmission, respectively. Taken together, our developed one-tube RPA-CRISPR-Cas12b-based mcr-1 and tet(X4) detection systems show promising potential for the onsite detection of mcr-1 and tet(X4). Key points • One-tube RPA-CRISPR-Cas12b-based mcr-1 and tet(X4) detection systems were developed based on identified novel protospacers. • Both detection systems exhibited high sensitivity and specification with a sample-to-answer time of less than 1 h. • The detection systems show promising potential for onsite detection of mcr-1 and tet(X4).

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International Spread of Tet(X4)-Producing Escherichia coli Isolates

Cited by 9 publications

References 20 publications

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Rapid detection of multiple resistance genes to last-resort antibiotics in Enterobacteriaceae pathogens by recombinase polymerase amplification combined with lateral flow dipstick

Development and evaluation of rapid and accurate one-tube RPA-CRISPR-Cas12b-based detection of mcr-1 and tet(X4)

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