Multiple insecticide resistance in Culex quinquefasciatus populations from Guadeloupe (French West Indies) and associated mechanisms

Delannay, Christelle; Goindin, Daniella; Kellaou, Kevin; Ramdini, Cédric; Gustave, Joël; Vega-Rúa, Anubis

doi:10.1371/journal.pone.0199615

Cited by 34 publications

(25 citation statements)

References 37 publications

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“…quinquefasciatus mosquito in the area of study would survive from temephos exposure. This result of study in accordance with previous study by Dellanay et al 12 which showed the temephos resistance status of the Tunisian Culex pipiens. This agrees with our results which showing a resistance status of Cx.…”

Section: Discussionsupporting

confidence: 93%

Temephos Resistance in Culex quinquefasciatus Population from Pabean Subdistrict Pekalongan

Widiastuti¹,

Sunaryo²,

Wijayanti

2019

aspirator

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Abstrak. Pekalongan adalah satu di antara daerah dengan kejadian filariasis limfatik sejak beberapa tahunyang lalu dan Culex quinquefasciatus merupakan vektor utama penyakit ini di Pekalongan. Penelitian inibertujuan untuk mengetahui status kerentanan terhadap temephos dan menggambarkan aktivitas esterasepada populasi Cx. quinquefasciatus di Pekalongan. Penelitian dilakukan pada bulan April-Juli 2018. Ujikerentanan insektisida dilakukan pada larva tangkapan liar Cx. quinquefasciatus dari Pekalongan dengandosis temephos yang diskriminatif (0,02 ppm). Aktivitas Esterase larva ini diuji secara biokimia untukmempelajari mekanisme resistensi. Pada semua ulangan, angka kematian akibat temephos bervariasi dari70% sampai 75%, menunjukkan bahwa Cx. quinquefasciatus di tempat penelitian resisten terhadaptemephos. Hasil uji biokimia menunjukkan peningkatan aktivitas alfa dan beta esterase pada populasi Cx.quinquefasciatus. Hasil yang disajikan di sini memberikan laporan dan informasi dasar tentang statusresistensi terhadap temephos pada Cx. quinquefasciatus di Pekalongan. Hal ini seharusnya menjadiperhatian bagi dinas kesehatan dalam manajemen pengendalian vektor.

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Section: Discussionsupporting

confidence: 93%

Temephos Resistance in Culex quinquefasciatus Population from Pabean Subdistrict Pekalongan

Widiastuti¹,

Sunaryo²,

Wijayanti

2019

aspirator

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“…The Leu/Phe substitution and prior reports of the kdr mutation have been implicated in the development of pyrethroid resistance in several mosquito species, including An. gambiae [32], Culex pipiens pallens [33] and Culex pipiens quinquefasciatus [34, 35]. Previous researches have found high frequencies of 1014F/C and 1014F/S substitutions in An.…”

Section: Discussionmentioning

confidence: 99%

The polymorphism and geographical distribution of knockdown resistance of adult Anopheles sinensis populations in eastern China

Tan

et al. 2019

Malar J

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Background Anopheles sinensis is one of the major malaria vectors in China and other southeast Asian countries, including Vietnam, Cambodia, Thailand. Vector control is considered to be the critical measure for malaria control, while the increasing prevalence of insecticide resistance caused by long-term use of insecticides, especially pyrethroids, is threatening the successful control of An. sinensis . In order to understand the underlying resistance mechanisms involved and molecular basis, the principal malaria vector, An. sinensis from Jiangsu and Anhui provinces, Southeast China, was investigated. Methods The adult Anopheles mosquitoes were sampled from multiple sites across Jiangsu and Anhui provinces, and sufficient mosquitoes collected from eleven sites for insecticide susceptibility bioassays. The DIIS4–DIIS6 region of the para -type sodium channel gene was amplified and sequenced, then multiple PCR and Taqman assays were used to assess the frequencies of kdr mutations at the target gene. Results In the present study, most of the adult An. sinensis populations were pyrethroids resistant, which indicated the presence of kdr resistance mutations in the para -type sodium channel gene. Sequence analyses demonstrated the kdr mutation existed at codon 1014 in Jiangsu and Anhui provinces. In adult An. sinensis , three mutant types (TTT L1014F, TTC L1014F, and TGT L1014C) of kdr alleles were detected, while no wild type (TTG L1014) was observed. The TTC L1014F mutation was first reported in Anhui province. Conclusions The highly polymorphic kdr alleles were observed in all the adult An. sinensis populations, which suggested that in-depth studies are required for carrying on insecticide resistance monitoring and specific resistance mechanisms studying into establish effective long-term malaria vector control program in eastern China.

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“…Similar results encasing pyrethroid resistance in Cx . quinquefasciatus have been reported worldwide [32–35]. Resistance to pyrethroids in Cx .…”

Section: Discussionmentioning

confidence: 99%

Insecticide resistance mapping in the vector of lymphatic filariasis, Culex quinquefasciatus Say from northern region of West Bengal, India

et al. 2019

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Culex quinquefasciatus is a vector of lymphatic filariasis and vector control strategies normally involve the use of synthetic insecticides targeted against them. Extensive and uncontrolled use of these synthetic insecticides has led to the development of insecticide resistance in the mosquito vectors. In this context, to study the resistance status of Cx . quinquefasciatus , field populations were collected from three districts of Northern part of West Bengal and tested against insecticides (5% malathion, 0.05% deltamethrin, 0.05% lambdacyhalothrin,0.75% permethrin, 0.1% propoxur, 4% DDT and Temephos). Qualitative and quantitative enzyme assay was also conducted in order to find the role of detoxifying enzymes behind the development of insecticide resistance. This study revealed the presence of widespread resistance amongst the field populations of Cx . quinquefasciatus throughout the studied regions. Moreover, the result of native PAGE and biochemical enzyme assay may be linked to some extent in the involvement of the detoxifying enzymes in conferring resistance against insecticides in most of the tested Cx . quinquefasciatus populations. The present study involving the survey of resistance status may be of immense help during the implementation of vector control strategies throughout this region.

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Multiple insecticide resistance in Culex quinquefasciatus populations from Guadeloupe (French West Indies) and associated mechanisms

Cited by 34 publications

References 37 publications

Temephos Resistance in Culex quinquefasciatus Population from Pabean Subdistrict Pekalongan

Temephos Resistance in Culex quinquefasciatus Population from Pabean Subdistrict Pekalongan

The polymorphism and geographical distribution of knockdown resistance of adult Anopheles sinensis populations in eastern China

Insecticide resistance mapping in the vector of lymphatic filariasis, Culex quinquefasciatus Say from northern region of West Bengal, India

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