Multiple Infection of Cell Monolayers by Virus Mixtures

Assessment of Aerosol Mixtures of Different Viruses

“…The feasibility of employing selective immunofluorescence procedures in conjunction with fluorescent cell counting assays for estimating the concentration of each virus in aerosol mixtures was established by this study. The broad applicability and advantages of this procedure have been cited earlier (6,8). Additional considerations of the immunofluorescent assessment method relevant to characterizing the aerosol behavior of viruses are (i) that interpretation of results may be facilitated because the basis of assay for each virus in mixture is the same, (ii) that virus assessment of aerosol samples may be accomplished within 24 hr, (iii) that a more valid comparison of the aerosol behavior of viruses may be obtained because each virus in an aerosol mixture is subjected to identical stresses and test conditions, and (iv) that the aerosol behavior of several viruses may be studied simultaneously.…”

“…Recently, a rapid and highly specific procedure was developed for the assay of mixtures of viruses in suspension (6). The procedure is based on selective immunofluorescent staining in conjunction with fluorescent cell counting of cell monolayers infected by virus mixtures.…”

Multiple Assessment and Serum Neutralization of Arbovirus Mixtures

“…Selective immunofluorescence staining of infected cell monolayers in conjunction with fluorescent cell counting has been used to assay quantitatively mixtures of three or more viral agents (1). This assessment procedure is specific, rapid (less than 24 hr), and versatile in application for conducting experimental investigations that involve more than one virus.…”