Multiple cis-Acting Targeting Sequences Are Required for orb mRNA Localization During Drosophila Oogenesis

Lantz, Valerie; Schedl, Paul

doi:10.1128/mcb.14.4.2235-2242.1994

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…For the experiment, we selected the 3′UTR from the orb gene, which encodes the other Drosophila CPEB protein. Like orb2, its mRNA has localization signals and it also has functions in LTM formation [ 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…The same picture was observed in orb2 orb flies-i.e., their STM and MTM formation was normal (Figure 1B). Thus, we found that different sequences of the 3′UTR of the orb2 mRNA did To provide further evidence that the proper localization/regulation of orb2 mRNA is critical for LTM formation, we replaced the deletion with two different UTR sequences, the αTub84B tubulin gene 3 UTR, which contains a single consensus CPE sequence, and the 3 UTR that belongs to the orb, another CPEB gene, and contains eight CPEs [34,38]. The full sequences are presented in Materials and Methods.…”

Section: Introduction Of Different Sequences In Orb2 R Allele Does No...mentioning

confidence: 99%

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3′UTR of mRNA Encoding CPEB Protein Orb2 Plays an Essential Role in Intracellular Transport in Neurons

Kozlov

Деев

Tokmatcheva

et al. 2023

Cells

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Intracellular trafficking plays a critical role in the functioning of highly polarized cells, such as neurons. Transport of mRNAs, proteins, and other molecules to synaptic terminals maintains contact between neurons and ensures the transmission of nerve impulses. Cytoplasmic polyadenylation element binding (CPEB) proteins play an essential role in long-term memory (LTM) formation by regulating local translation in synapses. Here, we show that the 3′UTR of the Drosophila CPEB gene orb2 is required for targeting the orb2 mRNA and protein to synapses and that this localization is important for LTM formation. When the orb2 3′UTR is deleted, the orb2 mRNAs and proteins fail to localize in synaptic fractions, and pronounced LTM deficits arise. We found that the phenotypic effects of the orb2 3′UTR deletion were rescued by introducing the 3′UTR from the orb, another Drosophila CPEB gene. In contrast, the phenotypic effects of the 3′UTR deletion were not rescued by the 3′UTR from one of the Drosophila α-tubulin genes. Our results show that the orb2 mRNAs must be targeted to the correct locations in neurons and that proper targeting depends upon sequences in the 3′UTR.

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Section: Introductionmentioning

confidence: 99%

Section: Introduction Of Different Sequences In Orb2 R Allele Does No...mentioning

confidence: 99%

3′UTR of mRNA Encoding CPEB Protein Orb2 Plays an Essential Role in Intracellular Transport in Neurons

Kozlov

Деев

Tokmatcheva

et al. 2023

Cells

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To be or not to be: orb, the fusome and oocyte specification in Drosophila

Barr,

Diegmiller,

Colonnetta

et al. 2024

Genetics

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In the fruit fly Drosophila melanogaster, two cells in a cyst of 16 interconnected cells have the potential to become the oocyte, but only one of these will assume an oocyte fate as the cysts transition through regions 2a and 2b of the germarium. The mechanism of specification depends on a polarized microtubule network, a dynein dependent Egl:BicD mRNA cargo complex, a special membranous structure called the fusome and its associated proteins, and the translational regulator orb. In this work, we have investigated the role of orb and the fusome in oocyte specification. We show here that specification is a stepwise process. Initially, orb mRNAs accumulate in the two pro-oocytes in close association with the fusome. This association is accompanied by the activation of the orb autoregulatory loop, generating high levels of Orb. Subsequently, orb mRNAs become enriched in only one of the pro-oocytes, the presumptive oocyte, and this is followed, with a delay, by Orb localization to the oocyte. We find that fusome association of orb mRNAs is essential for oocyte specification in the germarium, is mediated by the orb 3’ UTR, and requires Orb protein. We also show that the microtubule minus end binding protein Patronin functions downstream of orb in oocyte specification. Finally, in contrast to a previously proposed model for oocyte selection, we find that the choice of which pro-oocyte becomes the oocyte does not seem to be predetermined by the amount of fusome material in these two cells, but instead depends upon a competition for orb gene products.

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Mutational Analysis of an RNA Recognition Element That Mediates Localization of bicoid mRNA

Macdonald

Kerr

1998

Molecular and Cellular Biology

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Localization signals are RNA regulatory elements that direct the localization of mRNAs to subcellular sites. Localization signals presumably function by mediating RNA recognition events through which the mRNA becomes associated with the localization machinery. At present little is known about individual RNA recognition events, which in turn has limited progress in identifying the trans-acting binding factors involved in these events. Here we describe a detailed characterization of the RNA elements required for the RNA recognition event, event A, that initiates localization of bicoid mRNA in the Drosophila ovary. One element is a helix in which nucleotide identities are not important, suggesting that it plays a primarily structural role. Immediately adjacent to the helix is a recognition domain in which the identities of some, but not all, nucleotides are important for function. Comparison of two related but different RNAs that both support recognition event A further defines the important features of the recognition domain.

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Multiple cis-Acting Targeting Sequences Are Required for orb mRNA Localization During Drosophila Oogenesis

Cited by 4 publications

References 33 publications

3′UTR of mRNA Encoding CPEB Protein Orb2 Plays an Essential Role in Intracellular Transport in Neurons

3′UTR of mRNA Encoding CPEB Protein Orb2 Plays an Essential Role in Intracellular Transport in Neurons

To be or not to be: orb, the fusome and oocyte specification in Drosophila

Mutational Analysis of an RNA Recognition Element That Mediates Localization of bicoid mRNA

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