Multiple Functions of ATG8 Family Proteins in Plant Autophagy

Bu, Fan; Yang, Mingkang; Guo, Xu; Huang, Wei; Chen, Liang

doi:10.3389/fcell.2020.00466

Cited by 70 publications

(63 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In plants, the number of Atg8 orthologs varies from 1 in algae to 22 in angiosperms [74]; however, the diversity of plant Atg8 proteins could be significantly higher because of multiple gene duplications in order to adapt to various adverse conditions where Atg8 proteins play a crucial role [75]. The plant Atg8 proteins also reveal significant selectivity in interaction with their interaction partners, originated from the sequence difference between Atg8 isoforms in different species [76].…”

Section: Lessons From Alignment Of Atg8 Family Membersmentioning

confidence: 99%

Atg8-Family Proteins—Structural Features and Molecular Interactions in Autophagy and Beyond

Wesch

Kirkin

Rogov

2020

Cells

View full text Add to dashboard Cite

Autophagy is a common name for a number of catabolic processes, which keep the cellular homeostasis by removing damaged and dysfunctional intracellular components. Impairment or misbalance of autophagy can lead to various diseases, such as neurodegeneration, infection diseases, and cancer. A central axis of autophagy is formed along the interactions of autophagy modifiers (Atg8-family proteins) with a variety of their cellular counter partners. Besides autophagy, Atg8-proteins participate in many other pathways, among which membrane trafficking and neuronal signaling are the most known. Despite the fact that autophagy modifiers are well-studied, as the small globular proteins show similarity to ubiquitin on a structural level, the mechanism of their interactions are still not completely understood. A thorough analysis and classification of all known mechanisms of Atg8-protein interactions could shed light on their functioning and connect the pathways involving Atg8-proteins. In this review, we present our views of the key features of the Atg8-proteins and describe the basic principles of their recognition and binding by interaction partners. We discuss affinity and selectivity of their interactions as well as provide perspectives for discovery of new Atg8-interacting proteins and therapeutic approaches to tackle major human diseases.

show abstract

Section: Lessons From Alignment Of Atg8 Family Membersmentioning

confidence: 99%

Atg8-Family Proteins—Structural Features and Molecular Interactions in Autophagy and Beyond

Wesch

Kirkin

Rogov

2020

Cells

View full text Add to dashboard Cite

show abstract

“…These results illustrated a higher similarity with homology proteins in tea plant than in Arabidopsis , and CsATG8e showed more sequence similarity to ATG8c in both Arabidopsis and Camellia sinensis . Based on the amino acid sequences, ATG8 proteins can be divided into three groups: clade I (a), clade I (b) and clade II [ 33 ]. However, according to our phylogenetic analysis of CsATG8s ( Figure 2 ), no clear classification was found for CsATG8s in tea plant.…”

Section: Resultsmentioning

confidence: 99%

“…In crops like rice [ 42 , 49 , 50 ] and soybean [ 51 ], a number of members in the ATG8 subfamily have been shown to participate in N utilization. Previous studies have shown that the ATG9 complex and ubiquitin-like conjugation pathways are indispensable in the process of autophagy and NBR1 is a typical receptor for selective autophagy [ 25 , 33 ]. In this study, the expressions of AtATG9 , AtNBR1 and 9 genes in ubiquitin-like conjugation pathways were found to be upregulated in OE lines versus WT ( Figure 6 a,d), indicating the enhanced autophagy in CsATG8e transgenic plants.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of CsATGs in Tea Plant and the Involvement of CsATG8e in Nitrogen Utilization

Huang

Liu

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Nitrogen (N) is a macroelement with an indispensable role in the growth and development of plants, and tea plant (Camellia sinensis) is an evergreen perennial woody species with young shoots for harvest. During senescence or upon N stress, autophagy has been shown to be induced in leaves, involving a variety of autophagy-related genes (ATGs), which have not been characterized in tea plant yet. In this study, a genome-wide survey in tea plant genome identified a total of 80 Camellia Sinensis autophagy-related genes, CsATGs. The expression of CsATG8s in the tea plant showed an obvious increase from S1 (stage 1) to S4 (stage 4), especially for CsATG8e. The expression levels of AtATGs (Arabidopsis thaliana) and genes involved in N transport and assimilation were greatly improved in CsATG8e-overexpressed Arabidopsis. Compared with wild type, the overexpression plants showed earlier bolting, an increase in amino N content, as well as a decrease in biomass and the levels of N, phosphorus and potassium. However, the N level was found significantly higher in APER (aerial part excluding rosette) in the overexpression plants relative to wild type. All these results demonstrated a convincing function of CsATG8e in N remobilization and plant development, indicating CsATG8e as a potential gene for modifying plant nutrient utilization.

show abstract

“…In general, the expression of the BnATG8 subfamily under all abiotic stresses was higher than other subfamilies, which is the following results obtained in Arabidopsis [ 38 ], foxtail millet [ 50 ], and wheat [ 38 , 50 , 71 ]. ATG8 is a ubiquitin-like protein conjugated to phosphatidylethanolamine (PE) catalyzed by ATG7, ATG3, and ATG12-ATG5 complex [ 72 ]. ATG8-PE complex is essential for autophagosome formation through the membrane connection and remodeling [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

In Silico Analyses of Autophagy-Related Genes in Rapeseed (Brassica napus L.) under Different Abiotic Stresses and in Various Tissues

Eshkiki

Hajiahmadi

Abedi

et al. 2020

Plants

View full text Add to dashboard Cite

The autophagy-related genes (ATGs) play important roles in plant growth and response to environmental stresses. Brassica napus (B. napus) is among the most important oilseed crops, but ATGs are largely unknown in this species. Therefore, a genome-wide analysis of the B. napus ATG gene family (BnATGs) was performed. One hundred and twenty-seven ATGs were determined due to the B. napus genome, which belongs to 20 main groups. Segmental duplication occurred more than the tandem duplication in BnATGs. Ka/Ks for the most duplicated pair genes were less than one, which indicated that the negative selection occurred to maintain their function during the evolution of B. napus plants. Based on the results, BnATGs are involved in various developmental processes and respond to biotic and abiotic stresses. One hundred and seven miRNA molecules are involved in the post-transcriptional regulation of 41 BnATGs. In general, 127 simple sequence repeat marker (SSR) loci were also detected in BnATGs. Based on the RNA-seq data, the highest expression in root and silique was related to BnVTI12e, while in shoot and seed, it was BnATG8p. The expression patterns of the most BnATGs were significantly up-regulated or down-regulated responding to dehydration, salinity, abscisic acid, and cold. This research provides information that can detect candidate genes for genetic manipulation in B. napus.

show abstract

Multiple Functions of ATG8 Family Proteins in Plant Autophagy

Cited by 70 publications

References 115 publications

Atg8-Family Proteins—Structural Features and Molecular Interactions in Autophagy and Beyond

Atg8-Family Proteins—Structural Features and Molecular Interactions in Autophagy and Beyond

Genome-Wide Identification of CsATGs in Tea Plant and the Involvement of CsATG8e in Nitrogen Utilization

In Silico Analyses of Autophagy-Related Genes in Rapeseed (Brassica napus L.) under Different Abiotic Stresses and in Various Tissues

Contact Info

Product

Resources

About